Molecular motor lipid vesicle composition as well as preparation method and application thereof

A technology of molecular motors and lipid vesicles, which is applied in the field of biological and polymer materials, can solve the problems of poor mechanical properties, limited life, and easy breakage, and achieve the effects of improving stability, improving delicate vitality, and promoting wound healing

Active Publication Date: 2021-04-16
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a method to improve the stability of the lipid membrane in order to solve the traditional bilayer membrane, poor mechanical pr

Method used

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  • Molecular motor lipid vesicle composition as well as preparation method and application thereof
  • Molecular motor lipid vesicle composition as well as preparation method and application thereof

Examples

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preparation example Construction

[0051]The present invention also provides a method of preparing a molecular motor lipid vesicle, the step of preparing:

[0052]1) Collection of bacteria;

[0053]The bacteria used in step 1) include a kind of thermophilic fungi, thermophilic bacteria, Beltophili, Bacillus, Slimophilic acid Soclastin, Slimophili, and Soilophilus.

[0054]Preferably, theophilic bacteria are thermophilicin.

[0055]2) Bacterial crushing: The bacterium obtained by step 1) is broken to obtain a cell fragmer;

[0056]The bacterial crush used in step 2) can be mechanically, for example: high-pressure homogenization method, oscillating bead crushing method, high speed mixing bead grinding method, ultrasonic crushing method, hit crushing method; or non-mechanical method, for example Osmotic pressure impact crushing method, freeze-thaw crushing method, enzyme-dissolving method, chemical crushing method.

[0057]In the present invention, in order to improve the anti-oxidation resistance of lipid vesicles, the lipid peroxidatio...

Embodiment 1

[0072]This embodiment provides a method of preparing a molecular motor lipid vesicle, and the preparation method thereof comprises:

[0073]1) Bacterial collection: In the culture of Botherophilic bacteria, cultured in 75 ° C to the latter period, collected bacteria;

[0074]2) Crushing of the bacteria: After the bacterium obtained in step 1) was preserved by -80 ° C, 500 g of the bacterium was taken into 1 liter buffer (80 mm Tris.HCl, pH 8.0, including 1.0 mMβ-thiol). Ethanol, 0.Lmm EDTA 0.25M sucrose, 4mmmmgCl2The solution is suspended, treated with ultrasonic treatment to achieve crushing;

[0075]3) Centrifugation, prepared heat-resistant SOD: centrifuge at high speed (20000 g) for 30 min, resulting in precipitate A and supernatant B. The precipitate A was added with 500 ml of buffer (15 mM Tris.HCl, pH 7.6, including 1.0 mMβ-mercaptoethanol, 0.LmMedta) to 20 m mgCl2The solution was 8 ml and DNasei 10 mg, and the reaction was reacted for 30 minutes under room temperature. After the DNas...

Embodiment 2

[0080]The present embodiment is the same as in Example 1, and the only difference is that the polyglymine is replaced with polyethyleneiocine.

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Abstract

The invention relates to a molecular motor lipid vesicles composition, a preparation method and an application of the composition. The composition has the effects of improving the solubility and long-term effect of molecular motors and promoting absorption, and the assembly structure of the molecular motor lipid vesicles is a closed structure or a cell compartment containing molecular motor ATP synthase and lipid bilayers thereof and having biological membrane properties, the molecular motor lipid vesicles called vesicles, niosolnes are also called liposomes in the past, the biological membrane containing the molecular motor has a lipid bilayer structure, so that the biological membrane has moisturizing, whitening and anti-aging effects when being applied to the field of cosmetics, achieves the effects of improving the stability, improving the solubility and the long-term effect of the molecular motor and promoting absorption, and is simple and convenient in production process, and low in cost, and the defects that the lipidosome adopting phospholipid needs to be purified, is easy to oxidize and degrade and the like are avoided.

Description

Technical field[0001]The present invention belongs to the field of biological and polymer materials, and more particularly to the composition of molecular motor lipid vesicles for improving the solubility, long-termness of the molecular motor and promoting the action of the drug or efficacy component and the preparation method thereof.Background technique[0002]The molecular motor is made of biological molecules and is used by chemical energy. Usually a class of ATP synthase proteins is the use of transmembrane electrochemical gradient to catalyze the synthesis of the membrane protein composite family of ATP, and the transmembrane proton retardation is used to synthesize ATP. In the organism, the small molecules of the saccharide and fats in the mitochondria were oxidized in the mitochondria, and most of these energy is stored in a molecule called adenosine triphosphate (ATP) to be bio-utilized. .[0003]The molecular motor is also a group of transmembrane proteins, typically embedded ...

Claims

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Application Information

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IPC IPC(8): A61K8/14A61K8/64A61K8/66A61K8/88A61K8/55A61Q19/00A61Q19/02A61Q19/08
Inventor 魏建良颜贵卉王旻子张明洲姚雨辰章鹏坤
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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