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Antibacterial peptide compound, antibacterial peptide extract, and preparation method and application thereof

A technology of antimicrobial peptides and compounds, applied in the field of industrial microorganisms, can solve problems such as drug residues in drug-resistant strains and threats to human health, and achieve the effects of novel structure, low energy consumption, and clear functional components

Active Publication Date: 2021-04-16
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the food, medicine and animal husbandry industries, due to the extensive use of traditional antibiotics, the emergence of a large number of drug-resistant strains and drug residues has caused a great threat to human health

Method used

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  • Antibacterial peptide compound, antibacterial peptide extract, and preparation method and application thereof
  • Antibacterial peptide compound, antibacterial peptide extract, and preparation method and application thereof
  • Antibacterial peptide compound, antibacterial peptide extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 prepares antimicrobial peptide fermented liquid

[0041] 1) Activation of strains on the slant: Inoculate the refrigerated tube strain of Brevibacillus lateralosporus in LB slant medium and culture at 35°C for 3 days.

[0042] 2) Seed shake flask culture: insert the slant culture into the sterilized seed medium, and culture it at 35 degrees, 200 rpm, for 8 hours in the shake flask to obtain seed liquid, morphological Brevibacterium, with a cell concentration of 7*107cfu / ml;

[0043] The seed medium is prepared as follows: yeast extract 10g corn steep liquor powder 15g glucose 30g sodium chloride 5g potassium dihydrogen phosphate 1g calcium carbonate 5g, tap water 1000ml, pH 6.0, 121 degrees, sterilized for 30 minutes;

[0044] 3) Fermentation culture:

[0045] The seed solution obtained in step 2) is inoculated into the fermentation medium with an inoculum amount of 2%.

[0046] The fermentation medium was prepared as follows:

[0047] Glucose 30g glycer...

Embodiment 2

[0049] Embodiment 2 prepares antimicrobial peptide fermented liquid

[0050] 1) Activation of strains: Inoculate the refrigerated tube strains of Brevibacillus lateralosporus in LB slant medium, and culture at 32°C for 3 days.

[0051] 2) Seed shake flask culture: insert the slant culture into the sterilized seed medium, culture at 32 degrees, 200rpm, for 16hr, and obtain the seed liquid, the shape of Brevibacterium, the cell concentration is 5*107cfu / ml;

[0052]The seed medium is prepared as follows: yeast extract 20g corn steep liquor powder 5g glucose 20g sodium chloride 3g potassium dihydrogen phosphate 0.1g calcium carbonate 2g, tap water 1000ml, pH 7, 121 degrees, sterilized for 30 minutes;

[0053] 3) Fermentation culture:

[0054] The seed solution obtained in step 2) is inoculated into the fermentation medium with an inoculum size of 10%.

[0055] The fermentation medium was prepared as follows:

[0056] Glucose 20g glycerin 20g ammonium sulfate 4g yeast powder 10...

Embodiment 3

[0058] Embodiment 3 prepares antimicrobial peptide fermented liquid

[0059] 1) Activation of strains: Inoculate the refrigerated strains of Brevibacillus spp. in LB slant medium, and culture them at 37°C for 3 days.

[0060] 2) Seed shake flask culture: insert the slant culture into the sterilized seed medium, cultivate at 37 degrees, 200 rpm, for 8 hours, and obtain the seed liquid, Brevibacterium, with a cell concentration of 8.4*107cfu / ml;

[0061] The seed medium is prepared as follows: yeast extract 5g corn steep liquor powder 20g glucose 20g sodium chloride 10g potassium dihydrogen phosphate 0.5g calcium carbonate 3g, tap water 1000ml, pH 6.5, 121 degrees, sterilized for 30 minutes;

[0062] 3) Seed tank cultivation: the volume of the tank is 300L, and the filling capacity is 150L; the culture conditions are 37 degrees, the rotation speed is 40hz, the air flow rate is 1:1.2, and the tank pressure is 0.05MPa. The medium is the same as the seed bottle seed medium. After...

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Abstract

The invention discloses an antibacterial peptide compound. The antibacterial peptide compound has a structure shown as a formula (I). The invention also provides a preparation method of an extract taking the compound as a main component and an application of the extract in resisting bacteria and fungi. The compound disclosed by the invention is novel in structure, simple in extract preparation process, low in energy consumption and high in product purity, and the obtained antibacterial peptide compound and extract have a good antibacterial effect.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganisms, and relates to an antimicrobial peptide compound and an extract, a preparation method and application thereof. [0002] technical background [0003] In the food, medicine, and animal husbandry industries, due to the extensive use of traditional antibiotics, a large number of drug-resistant strains and drug residues have emerged, posing a great threat to human health. Moreover, with the improvement of people's health consumption awareness, there is an urgent need to develop new natural antibacterial substances that are not easy to produce drug resistance, have no residue, and have no potential safety hazards. Antimicrobial peptides from natural sources have become a research hotspot in recent years and have been widely used in food, medicine and animal husbandry industries. [0004] Antimicrobial peptides are derived from a variety of organisms and are part of the natural defens...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/36C07K1/34C07K1/30C07K1/16A23K20/147A23L3/3526A61K38/10A61P31/04A61P31/10A61K8/64A61Q19/00A61Q17/00
Inventor 李丽红任风芝张雪霞郄丽萍段银箫朱京童封玉彬王哲米文强孔德清
Owner NCPC NEW DRUG RES & DEV
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