Strain of avian bacillus velezensis CL-4 and application thereof

A kind of poultry-derived Velez and Bacillus technology, applied in the application, bacteria, microorganism and other directions, to achieve the effect of safe use, no toxic side effects, and broad application value

Active Publication Date: 2021-04-16
JILIN ACAD OF AGRI SCI
View PDF14 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of animal husbandry, there have been no reports on Bacillus Veles with high hemicellulase production and the use of Bacillus Veles to ferment corn germ meal to degrade hemicellulose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain of avian bacillus velezensis CL-4 and application thereof
  • Strain of avian bacillus velezensis CL-4 and application thereof
  • Strain of avian bacillus velezensis CL-4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation, screening and identification of Bacillus velezensis CL-4

[0029] 1. Medium

[0030] LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water to 1000mL, pH 7.0, autoclaved at 121°C for 20min.

[0031] LB solid medium: add 15-20g agar powder to LB liquid medium, and autoclave at 121°C for 20min.

[0032] LB slant solid medium: add 15-20g agar powder to LB liquid medium, and autoclave at 121°C for 20min.

[0033] Xylanase screening medium: xylan 1g, soybean peptone 0.3g, tryptone 1.7g, NaCl 0.5g, glucose 0.25g, potassium dihydrogen phosphate 0.25g, agar 2g, distilled water to 100mL, pH 7.0, Autoclave at 121°C for 20 minutes.

[0034] Enzyme-producing fermentation medium: peptone 10g, yeast extract 5g, sodium chloride 10g, bran 10g, distilled water to 1000mL, pH 7.0, autoclaved at 121°C for 20min.

[0035] 2. Test method

[0036] (1) Isolated sample: cecal content of broiler chickens from Institute of Animal Nutrition...

Embodiment 2

[0056] Raw material pretreatment and preparation technology of embodiment 2 Bacillus velezensis (Bacillus velezensis) CL-4 fermented corn germ meal

[0057] 1. Materials

[0058] (1) Fermentation base material: corn germ meal, provided by Gongzhuling Huanglong Food Industry Co., Ltd.

[0059] (2) Strains: Bacillus velezensis CL-4 screened in Example 1 of the present invention, separated and preserved by the Institute of Animal Nutrition and Feed, Jilin Academy of Agricultural Sciences, Bacillus BLCC1-0157 and Bacillus BLCC1 -0155 was provided by the Strain Collection Center of Shandong Baolai Lilai Bioengineering Co., Ltd. Institute of Biology.

[0060] (3) Reagent: sodium bicarbonate.

[0061] 2. Test method

[0062] (1) Sterilization treatment of corn germ meal raw material: the original corn germ meal raw material was autoclaved at 121° C. for 20 minutes.

[0063] (2) Untreated raw material group (without adding sodium bicarbonate): Weigh a certain amount of high-pressu...

Embodiment 3

[0079] The preparation of embodiment 3 Veles bacillus (Bacillus velezensis) CL-4 bacterium powder

[0080] 1. Basic culture medium

[0081] The production of Bacillus velezensis CL-4 powder uses LB liquid medium as the basic medium.

[0082]2. Strains: Bacillus velezensis CL-4 obtained in Example 1 of the present invention was selected.

[0083] Slant culture: Bacillus velezensis CL-4 was inoculated on LB slant solid medium, and cultured at 37°C for 24 hours.

[0084] Primary seed culture: put the cultured CL-4 slant into 100mL LB liquid medium with an inoculation loop under sterile conditions, and cultivate it at 180rpm / min for 24 hours at 37°C to obtain the primary seed liquid.

[0085] 3. 50L seed tank fermentation

[0086] (1) culture medium

[0087] Tryptone 1% (mass percentage), yeast extract 0.5% (mass percentage), sodium chloride 1% (mass percentage), glucose 0.2% (mass percentage), initial pH 7.0, loading capacity 35L.

[0088] (2) Sterilization

[0089] Empty ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to avian bacillus velezensis CL4 and application thereof, and belongs to the field of agricultural animal husbandry application, the strain is preserved in China Center for Type Culture Collection on November 30, 2020, and the preservation number is CCTCC NO: M2020811. The avian bacillus velezensis CL-4 disclosed by the invention can be applied to the aspects of degrading hemicellulose and producing hemicellulase by fermenting corn germ meal. The invention further discloses a preparation method of the avian bacillus velezensis CL4. The avian bacillus velezensis CL-4 disclosed by the invention is safe to use and free from toxic and side effects, has the effects of degrading hemicellulose of the maize germ meal and improving the yield and activity of hemicellulase in the maize germ meal, and has a wide application value.

Description

technical field [0001] The invention belongs to the technical field of application of agriculture and animal husbandry, and in particular relates to a strain of Bacillus velezensis CL-4 of avian origin and its application. Background technique [0002] In recent decades, my country's pig feed formula has been based on Western countries, mainly based on "corn-soybean meal" rations, including the feed industry. According to statistics from the National Grain and Oil Information Center, my country's total soybean demand in 2019 reached 106.61 million tons Among them, the feed industry consumes about 70 million tons of soybean meal per year, but the domestic soybean production does not exceed 20 million tons per year. Due to the severe shortage of domestic protein source feed, my country's feed industry is overly dependent on soybean imports. Therefore, combined with my country's national conditions, vigorously developing and applying unconventional protein feed resources can ef...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/37C12R1/07
CPCY02P60/87
Inventor 陈龙魏炳栋于维郑琳闫晓刚李立佳张莹仲伟光祁宏伟
Owner JILIN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap