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Method for detecting respiratory system injury caused by PM2.5

A technology of respiratory system and detection method, which is applied in the field of biomedicine and can solve problems such as short exposure time

Pending Publication Date: 2021-04-16
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The non-patent literature "Particulate matter-induced senescence of skinkeratinocytes involves oxidative stress-dependent epigenetic modifications" studied PM 2.5 Exposure affects DNMT1, Dnmt3b and TET1 and then affects skin aging, but does not involve DNMT3A and respiratory system damage
[0005] In the non-patent literature "Repeated PM2.5 exposure inhibits BEAS-2B cell P53expression through ROS-Akt-DNMT3B pathway-mediated promoter hypermethylation", PM 2.5 Effects of exposure on DNA methylation, but results suggest that PM 2.5 It has an effect on DNMT1 and Dnmt3b, but has no obvious effect on the expression of DNMT3A, and PM 2.5 shorter exposure time

Method used

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  • Method for detecting respiratory system injury caused by PM2.5
  • Method for detecting respiratory system injury caused by PM2.5
  • Method for detecting respiratory system injury caused by PM2.5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1PM 2.5Experimental Design of Repeated Dose Exposure in SD Rats

[0086] Select SPF grade male SD rats, 6 weeks old, weighing 200±20g. Breeding conditions: 3 rats per cage, raised in the experimental animal room of Capital Medical University, changing the litter 2-3 times a week, free to eat and drink. The temperature of the experimental animal room is 24°C ± 1°C, the humidity is 50% ± 5%, the daily light time in the breeding room is 12h, and the ventilation is uninterrupted for 24h.

[0087] PM 2.5 The exposure dose is based on the PM given by WHO 2.5 The annual average concentration transition period target-1 (IT-1) limit, the rat's body weight, respiratory volume, respiratory rate and toxicological extrapolation safety factor are calculated (Table 1), unit body weight per day PM 2.5 The exposure dose was 1.8mg / kg, and the middle dose and high dose were determined to be 3 times and 9 times of the low dose, namely 5.4mg / kg and 16.2mg / kg.

[0088] Table 1 C...

Embodiment 2

[0199] Example 2 PM 2.5 Repeated dose exposure of human bronchial epithelial cells

[0200] (1) In this study, human bronchial epithelial cells (BEAS-2B) were used as an in vitro cell experimental model.

[0201] 1) Cell recovery: Before the experiment, the ultra-clean workbench was sterilized by ultraviolet light for 30 minutes, the required reagents were heated in a 37°C water bath, and an alcohol lamp was lit in the ultra-clean workbench. Take out the cells frozen in liquid nitrogen, quickly thaw them in a 37°C water bath, and transfer them to pre-warmed complete culture medium (DMEM containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin) , gently blow evenly with a pipette gun, centrifuge at 1200r / min for 3min at room temperature, discard the supernatant, resuspend the complete culture solution, plant it in a 100mm culture dish, and put it in a cell culture incubator at 37°C and 5% CO2 for cultivation. After 24 h, replace with new complete medi...

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Abstract

The invention provides a biomarker for respiratory system injury caused by PM2.5, a detection method for respiratory system injury caused by PM2.5 and application of the biomarker. The biomarker is DNA methylated transferase. According to the invention, the molecular mechanism of the PM2.5-induced respiratory system injury is explored from an epigenetic level, a theoretical basis and guiding significance are provided for detection of the PM2.5-induced respiratory system injury, and the molecular mechanism has important value in diagnosis of the PM2.5-induced respiratory system injury, screening of drugs for treating or preventing the PM2.5-induced respiratory system injury and providing of decision support for clinical treatment.

Description

technical field [0001] The present invention relates to the field of biomedicine, mainly relates to methyltransferase DNMT1, DNMT3A and DNMT3B in PM 2.5 Detection of respiratory damage. Background technique [0002] Atmospheric fine particulate matter (Fine Particulate Matter, PM 2.5 ) refers to atmospheric suspended particulate matter with an aerodynamic diameter ≤ 2.5 μm. PM 2.5 It is an extremely harmful component of air pollutants. Epidemiological studies have found that PM 2.5 For every 10 μg / m3 increase, all-cause mortality, pulmonary heart disease mortality, and lung cancer mortality increased by 4%, 6%, and 8% respectively; the current study found that PM 2.5 The mechanisms of damage to the respiratory system mainly include oxidative stress, inflammatory response, immune injury, DNA damage, cell cycle arrest, apoptosis, autophagy, and pyroptosis. However, the specific mechanism needs further research and discovery, and its in-depth study has a very high environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/573
Inventor 段军超孙志伟周显青于洋李洋
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES