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Application of crotonate, its preparation and its preparation method

A technology of crotonic acid salt and crotonic acid, which is applied in the field of application, preparation of its preparation and its preparation, can solve the problems of not improving the survival rate, etc., and achieve the effects of regulating myocardial cell death, reducing cardiac fibrosis, and repairing heart damage

Active Publication Date: 2022-01-28
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, other various vasoactive drugs in the prior art do not improve the survival rate compared with epinephrine

Method used

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  • Application of crotonate, its preparation and its preparation method
  • Application of crotonate, its preparation and its preparation method
  • Application of crotonate, its preparation and its preparation method

Examples

Experimental program
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Effect test

preparation example Construction

[0038] The embodiment of the present invention provides a preparation method of crotonate preparation, comprising:

[0039] The crotonic acid crystals are mixed with an organic solvent, and then ultrasonically crushed, so that the crotonic acid crystals are dissolved to form the crotonic acid solution.

[0040] The solvent used in the embodiment of the present invention is DMSO, but it can be understood that other solvents that can dissolve crotonic acid and will not affect the physiological and biochemical activities can also be used within the protection scope of the embodiment of the present invention.

[0041] The use of ultrasound is beneficial to the dissolution of crotonic acid, which is beneficial to the stability of the formed crotonic acid solution, and ensures that the crotonic acid is fully dissolved. At the same time, the use of crotonic acid crystals can reduce impurities in the crotonic acid solution and improve the therapeutic effect of the subsequently formed ...

Embodiment 1

[0051] The embodiment of the present invention provides a kind of preparation method of sodium crotonate solution, comprising:

[0052] Weigh 8.6 g of crotonic acid crystals, add 4 mL of DMSO solution, and dissolve all the crotonic acid crystals with the help of ultrasonic waves. At this time, the pH value of the crotonic acid solution is about 2.0 to 3.0, and the pH value is adjusted to 7.0 with NaOH solution. Add ultrapure water and titrate to 40ml to obtain a 2.5mol / L sodium crotonate solution. After aliquoting, freeze at -20°C and take it out when needed.

experiment example 1

[0053] Experimental Example 1: Proteomics Sequencing and Verification

[0054] Step 1: thoracotomy was performed on 6 C57 mice under inhalation anesthesia, 2 mice underwent thoracotomy without ligation; 4 mice ligated the left anterior descending coronary artery at 1 mm below the left atrium, and the ligation was released after 30 minutes. After confirming revascularization, the chest was closed. An ECG was connected intraoperatively to confirm successful modeling of myocardial ischemia-reperfusion injury. Disinfection, analgesia, liquid food, and heat preservation were given after the operation. Left atrium tissues were collected 3 days and 5 days after operation for protein mass spectrometry sequencing.

[0055] Step 2: Analyze proteomics data

[0056] Step 3: Validate the proteomics results

[0057] (1) if figure 1 Shown: After I / R surgery, proteomic results showed that about 60%-70% of sites with significant changes in crotonylation modification levels were concentrat...

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PUM

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Abstract

The invention relates to the field of application and preparation of medicines, in particular to the application of crotonate, its preparation and the preparation method of the preparation. The invention provides the application of crotonate in the preparation of medicine for treating heart disease. It can not only protect the acute and chronic heart damage, and then repair the heart damage, but also can be used for first aid of cardiac arrest, and then can prevent sudden cardiac death.

Description

technical field [0001] The invention relates to the field of application and preparation of medicines, in particular to the application of crotonate, its preparation and the preparation method of the preparation. Background technique [0002] At present, the number of patients with hypertension, coronary heart disease, and diabetes in our country is increasing day by day, and the problem of aging society is becoming more and more serious, which makes the prevalence of heart disease in our country show a rising trend. [0003] In 2014, the Cardiovascular Branch of the Chinese Medical Association released the "China Guidelines for the Diagnosis and Treatment of Heart Failure 2014". The "Golden Triangle" has become the basic treatment plan for various acute and chronic heart diseases. In recent years, with the wide application of Golden Triangle in the treatment of heart diseases, the mortality rate has been greatly reduced, but its mortality rate is still comparable to that o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/19A61P9/00A61P9/04C07C51/41C07C57/08
CPCA61K31/19A61P9/00A61P9/04C07C51/412C07C57/08
Inventor 段金柱蔡文倩曾锤许达才林应炯赵悦汪庆文董文艳
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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