Application of apelin protein in preparation of reagents for diagnosing respiratory diseases

A technology of respiratory diseases and reagents, applied in the field of medical testing, can solve the problems of serious illness of patients, reduce the mortality rate, increase the mortality rate, etc., and achieve the effect of improving the specificity of detection

Active Publication Date: 2022-04-08
温州医科大学慈溪生物医药研究院 +1
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  • Description
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Problems solved by technology

[0005] Although after nearly half a century of research, people have a deep understanding of the pathogenesis, pathophysiology, diagnosis and treatment of acute lung injury, but the mechanism of acute lung injury is very complex, involving infection, inflammation, immunity and tissue Most of the patients are already in the severe stage of acute lung injury when they see a doctor, which is very difficult in the clinical diagnosis and treatment. is less than 30 times / min), its oxygenation index is generally between 200-300mmHg, the body is not seriously hypoxic, and can basically meet the normal needs of organ functions or the body's various organ functions are in the compensatory period. The symptoms of patients in this stage are not typical, which are often ignored by clinicians. If they cannot be effectively identified and effective intervention measures are taken, patients tend to develop in a more serious direction and develop into the stage of ARDS with typical symptoms. serious, the mortality rate increased significantly
At present, there is still no effective method for early identification of acute lung injury and reduction of mortality in clinical practice

Method used

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  • Application of apelin protein in preparation of reagents for diagnosing respiratory diseases
  • Application of apelin protein in preparation of reagents for diagnosing respiratory diseases
  • Application of apelin protein in preparation of reagents for diagnosing respiratory diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Animal experiment modeling

[0024] Blind intestinal tangle penetration (CLP) induces the establishment of mice acute lung injury model

[0025] Adult C57 mouse (10), inject anesthesia mice with 100 μl of 1.5% pentobarbital, fixed to the operation board, the abdomen with pet electric pumping sharest, disinfecting the skin, do 1CM long incision in the abdomen , Ligation of the blindstone, puncture at the syringe of 26, and finally suture the wound and disinfect the skin. (See Daniel Rittirsch, Peter A Ward, etal.immunodesign of experimental sepsis by Cecal Ligation andPuncture.natprotoc.2009; 4 (1): 31-36)

[0026] Lipopolysaccharide, LPS induced the establishment of adult C57 mice (10), injecting anesthetized mice with 100 μL of 1.5% pentobarbital sodium abdominal cavity, fixed to the operation panel, pets with neck The electric push is razed, disinfecting the skin, does 1 cm long cut mouth in the neck, using a 1ml null needle to inject 50 uglps to the mice, the molding is ...

Embodiment 2

[0044] 12 patients with acute lung injury in the emergency lung injury in the first hospital of Wenzhou Medical University, and 12 cases of chronic obstructive pneumonia were used as a positive control (Note: All patients selected) were diagnosed. The exile serum specimens were taken by ELISA (Enzyme-linked immunosorbent assay) kit method to detect APELIN-13, GP73 and Cys-C, result in 12 cases of polar lung injury, APELIN-13, GP73 and CYS- C is more than 10 cases of normal value, and two of the two cases exceed normal value but Cys-C is normal. In patients with chronic obstructive pneumonia, as a positive control group, there were no 1 case, GP73, and Cys-C were simultaneously exceeding normal. This means that the diagnostic markers for acute lung injury are highly specific using Apelin-13, GP73 and Cys-C.

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Abstract

The invention discloses the application of Apelin protein in preparing reagents for diagnosing respiratory system diseases. The present invention is the first discovery that Apelin-13, especially the combination of Apelin-13, GP73 (Golgi apparatus transmembrane glycoprotein) and Cys-C (cystatin C) can be used as a diagnosis of respiratory diseases, especially the diagnosis of acute lung injury Markers can effectively improve the detection specificity of acute lung injury.

Description

Technical field [0001] The invention belongs to the field of medical detection, in particular the application of Apelin protein in the preparation of diagnosed respiratory disease reagents. Background technique [0002] Respiratory diseases are common diseases, multi-pathogenesis, main lesions in tracheal, bronchi, lungs, and chest, sorcerer, chest pain, respiratory affected, severe breathing, hypoxia, even respiratory failure. The mortality rate in the city accounts for the third place, while in the countryside. More attention is to pay attention to atmospheric pollution, smoking, population aging and other factors, so that the chronic obstructive pulmonary disease at home and abroad (referred to as chronic bronchitis, emphysema, pulmonary heart disease), bronchial asthma, lung cancer, Pulmonary diffuse measuring fibrosis, as well as the incidence of diseases such as pulmonary infection, and the mortality increases. [0003] According to the statistics of the death of some of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/74
CPCG01N33/6893G01N33/74G01N2333/8139G01N2333/47G01N2800/12G01N2800/125
Inventor 孔晓霞张宏宇肖健张海邻
Owner 温州医科大学慈溪生物医药研究院
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