Large-scale manufacturing method of diagnostic magnetic beads and biological application thereof
A manufacturing method and large-scale technology, applied in biochemical equipment and methods, microbiological measurement/inspection, DNA preparation, etc., can solve problems such as difficult to achieve large-scale manufacturing and cumbersome steps
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Embodiment 1
[0123] Example 1: Preparation method of diagnostic magnetic beads M1
[0124] 1) preparing magnetically cross-linked povidone particles;
[0125] 2) modifying the porous inorganic silica layer with the magnetic cross-linked povidone described in step 1 to prepare silicon-based magnetic cross-linked povidone particles;
[0126] 3) modifying the silicone layer on the silicon-based magnetic cross-linked povidone particles described in step 2 to prepare double-layer silicon-based magnetic cross-linked povidone particles.
[0127] Wherein, the specific synthesis steps of the cross-linked povidone particles in the step 1) include:
[0128] 1-1) 100 parts by mass of crospovidone particles with a particle size of 5-10 μm, crospovidone particles, 400 parts by mass of glycerin, 600 parts by mass of ethanol and 1000 parts by mass of water were mixed and mechanically stirred for 1 hour to disperse;
[0129] 1-2) After feeding nitrogen for 1 hour, add 100 parts by mass of ferrous ammoni...
Embodiment 2
[0138] Embodiment 2: the preparation method of diagnostic magnetic beads M2
[0139] 1) preparing magnetically cross-linked povidone particles;
[0140] 2) modifying the porous inorganic silica layer with the magnetic cross-linked povidone described in step 1 to prepare silicon-based magnetic cross-linked povidone particles;
[0141] 3) modifying the silicone layer on the silicon-based magnetic cross-linked povidone particles described in step 2 to prepare double-layer silicon-based magnetic cross-linked povidone particles.
[0142] Wherein, the specific synthesis steps of the cross-linked povidone particles in the step 1) include:
[0143] 1-1) 150 parts by mass of crospovidone particles with a particle size of 10-20 μm, 200 parts by mass of glycerin, 500 parts by mass of ethanol and 800 parts by mass of water are mixed and mechanically stirred for 1 hour until dispersed;
[0144] 1-2) After feeding nitrogen for 1 hour, add 150 parts by mass of ferrous ammonium sulfate, 280...
Embodiment 3
[0153] Embodiment 3: Magnetic leakage test of diagnostic magnetic beads M1, M2 and commercialized magnetic beads
[0154] The diagnostic magnetic beads M1, M2 provided in Example 1 and the MagneSil magnetic beads of Promega Company (product number MD1441), the Dynabeads MyOne of Thermo Company TM Silane (Product No. 37002D) was diluted with sterile water to 10mg / mL, and 1mL of the above-mentioned diluted magnetic bead suspension was taken. After repeated washing with deionized water for 3 times, 1mL of 0.5M HCl was added and reacted at room temperature for 1 hour at 1500rpm. The supernatant was absorbed by magnetic separation and the content of iron ions in the supernatant was detected by atomic absorption. Table 1 shows that the diagnostic magnetic beads M1 and M2 have low magnetic leakage, while the commercial magnetic beads MagneSil and MyOne TM The magnetic leakage of Silane is very obvious.
[0155] Table 1: Magnetic leakage test of different magnetic beads
[0156] ...
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