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Small molecule metabolite biomarker for diagnosing acute diseases as well as screening method and application of small molecule metabolite biomarker

A technology of small molecule metabolites and biomarkers, applied in the diagnosis model and construction of acute diseases, non-specific small molecule metabolites and their screening fields, can solve the problems of limited association information, unpredictable diseases, and insufficient diagnosis of acute diseases. basis, etc.

Pending Publication Date: 2021-05-04
哈尔滨脉图精准技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since clinical chemistry tests can only detect about 100 biochemical parameters on average, they can only provide information on a very small part of the metabolome with thousands of metabolites, and small molecule metabolites are nonspecific to a certain disease. There is limited information on the association between biochemical parameters and the diagnosed acute disease. In the absence of molecular marker data, it is not enough to be used as the basis for the diagnosis of an acute disease, let alone predict the disease

Method used

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  • Small molecule metabolite biomarker for diagnosing acute diseases as well as screening method and application of small molecule metabolite biomarker
  • Small molecule metabolite biomarker for diagnosing acute diseases as well as screening method and application of small molecule metabolite biomarker
  • Small molecule metabolite biomarker for diagnosing acute diseases as well as screening method and application of small molecule metabolite biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Relative Concentration Determination of Non-specific Small Molecule Metabolites

[0052] Simultaneous detection of thousands of metabolites in samples, samples from healthy individuals and diseased individuals, biological samples of healthy individuals include but not limited to blood, serum, plasma, urine, saliva, sweat, spinal fluid, respiratory, Feces, etc., samples from diseased individuals may further include biopsy tissue.

[0053] Blood samples from 100 lung cancer patients (LC), 100 lung chronic disease groups (LCD), 100 other cancer groups (OC) and 100 healthy individuals (Hctl) were obtained from the hospital (in patients and healthy individuals) after signing the informed consent form). Using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), the relative concentrations of small molecule metabolites in participants' blood samples were determined as follows.

[0054] 1.1. Sample processing

[0055] T...

Embodiment 2

[0088] Example 2: Screening of non-specific small molecule metabolite biomarkers for diagnosis of lung cancer

[0089]How to define the target (screening of biomarkers for diagnosing lung cancer) and how to preprocess the data is explained in detail in this example. Biomarker discovery implies the identification of metabolic signatures (i.e., small molecule metabolites) whose abundance (possibly several metabolites combined) in an individual is indicative of an individual's current or future disease or similar state under the condition. To this end, it is first necessary to screen for metabolic signatures with high predictive or diagnostic power (i.e., small molecule metabolite biomarkers), which can then be used to build supervised machine learning models, that is, to create models that can accurately predict unseen data. Models that respond to or diagnose data in individuals and can identify potential non-specific small molecule metabolites as biomarkers.

[0090] The chal...

Embodiment 3

[0118] Embodiment 3: Diagnostic equipment and diagnostic model of lung cancer

[0119] In this embodiment, a device for lung cancer diagnosis is also provided based on the provided lung cancer diagnosis method, which includes:

[0120] 1) an analysis unit for analyzing a subject sample comprising a detector (mass spectrometry device) for the determination of the amount of the small molecule metabolite biomarker determined in Example 2;

[0121]2) data storage unit, including the data obtained by the analysis unit of step 1);

[0122] 3) A data processing unit, including an algorithm for performing processing on the data in the data storage unit in step 2), specifically processing several small molecule metabolisms determined in Example 2 in the data storage unit in step 2). Quantitative data on biomarkers;

[0123] 4) An information output unit, which outputs the result processed by the data processing unit.

[0124] In this example, a lung cancer diagnosis model was also c...

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Abstract

The invention provides a small molecule metabolite biomarker for diagnosing acute diseases as well as a screening method and application thereof. The small molecule metabolites most related to the acute diseases is screened out to serve as the small molecule metabolite biomarker for diagnosing the acute diseases based on a method for simultaneously detecting thousands of small molecule metabolites in a biological sample. The invention further provides an application of establishing an acute disease diagnosis model and diagnosis equipment based on the small molecule metabolite biomarker, and provides an effective tool for early prediction, screening, intervention and treatment of acute diseases.

Description

technical field [0001] The invention belongs to the technical field of diagnosis and application of acute diseases, and in particular relates to non-specific small molecule metabolites for diagnosis of acute diseases and a screening method thereof, a diagnostic model of acute diseases using the non-specific small molecule metabolites as a biomarker and its construction method and application. Background technique [0002] Currently, health care costs are rising all over the world, partly due to unhealthy lifestyles and partly due to the growing proportion of middle-aged and elderly people. Therefore, controlling healthcare costs and providing guidance for a healthy lifestyle has become a common theme in all developed and developing countries. The core of achieving this goal is to find a technology that can meet the following needs: 1) It can perform early and executable diagnosis of individual acute diseases; 2) It can predict the possibility of a person suffering from a ce...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86G16B40/20G16H50/20
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/8679G01N30/7233G16B40/20G16H50/20
Inventor 李琰威尔米泽·洛萨·奥托·爱德华德
Owner 哈尔滨脉图精准技术有限公司
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