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Enzymatic-cell-rich piglet antibiotic-replacement feed composite additive and preparation method thereof

A compound additive, piglet technology, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of declining benefits, increasing disease rates, and raising breeding costs.

Pending Publication Date: 2021-05-07
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound additive rich in enzyme bacteria and anti-antibiotics for piglets and its preparation method, so as to solve the problem of decreased production performance, increased disease rate, increased breeding costs and declining benefits in the breeding process of piglets after antibiotics are banned And other issues

Method used

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  • Enzymatic-cell-rich piglet antibiotic-replacement feed composite additive and preparation method thereof
  • Enzymatic-cell-rich piglet antibiotic-replacement feed composite additive and preparation method thereof
  • Enzymatic-cell-rich piglet antibiotic-replacement feed composite additive and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 feeding probiotics

[0017] (1) Activation of bacteria

[0018] The probiotics used in the examples of the present invention were purchased from the Microorganism Collection Center, Bacillus subtilis CICC 24675 and Bacillus licheniformis CICC 20446.

[0019] The activated medium of Bacillus subtilis and Bacillus licheniformis adopts LB, and the formula is: yeast extract 0.5%, tryptone 1%, sodium chloride 1%, solid medium plus agar 2%. Inoculate the strain on the plate medium and culture it at 37°C for 24 hours to obtain activated strains;

[0020] (2) Fermentation culture

[0021] The activated strains of (1) were inoculated into liquid LB medium respectively to prepare 2L shake flask seeds, and then transferred to 50L and 400L primary and secondary fermenters, and cultivated for 12 hours. They were respectively transferred to three-stage 10T fermentation medium.

[0022] The formula of the fermentation medium is: 1% glucose, 1.5% cor...

Embodiment 2

[0025] The preparation of embodiment 2 feed enzyme preparations

[0026] The heterologous expression host bacteria of the enzyme preparations used in the examples of the present invention were all constructed in this laboratory.

[0027] (1) Preparation of seed solution

[0028] Pick a single colony of Pichia pastoris host bacteria for the feed enzyme preparation on the ultra-clean workbench, and make three-section lines on the plate to activate the strains. After a single colony grows, pick a single colony and inoculate it in 200mL YEPD medium with 1% yeast extract, 2% peptone, and 2% glucose, and culture at 30°C and 250rpm for 36-38h until the wet cell weight reaches 30g / L. Use 50 liters and 500 liters of fermenters for seed expansion culture respectively. The medium formula is yeast extract 1%, peptone 2%, glycerin 2%, 30°C, 350rpm, add 20% (w / v) ammonia solution to adjust pH ≈ 4.5 Fermentation under the conditions of fermentation, fermentation for about 24 hours, until t...

Embodiment 3

[0034] The preparation of embodiment 3 oligomannan oligosaccharides

[0035] (1) Preparation of hydrolyzate of konjac oligosaccharide

[0036] Use β-mannanase to hydrolyze konjac powder containing 25% in an enzymolysis tank at 60°C with an enzyme activity of 50IU / mL, stir and hydrolyze for 1 hour, heat up to 80°C, and keep warm for 10 minutes to terminate the enzymolysis reaction. After cooling, the crude fiber in the hydrolyzate is filtered and retained by a sieve.

[0037] (2) Filtration and concentration

[0038] First, the starch, coarse fiber particles and residues in the oligosaccharide solution are removed by high-speed centrifugation at 8000rpm, and then a small amount of protein, soluble starch, fiber and other particulate matter in the oligosaccharide solution is intercepted by a ceramic membrane with a pore size of 0.2 μm, and the pipeline is set at a pressure of 0.3MPa , add water in the middle and continue to circulate and wash and filter until the sugar content...

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Abstract

The invention discloses an enzymatic-cell-rich piglet antibiotic-replacement feed composite additive and a preparation method thereof. The composite additive is composed of probiotics, an enzyme preparation and prebiotics. The composite additive is added into a piglet feed according to a ratio of 500g / t, and can effectively replace piglet daily ration antibiotics. Compared with a basic daily ration, the piglet antibiotic-replacement feed added with the composite additive can improve the daily average feed intake and daily gain of piglets, reduce the diarrhea rate and the feed conversion ratio, and make the growth performance basically consistent with that of an antibiotic group; and the composite additive has good economic benefits and use values.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a compound additive rich in enzyme bacteria and anti-antibiotics for piglets and a preparation method thereof. Background technique [0002] Announcement No. 194 of the Ministry of Agriculture and Rural Affairs, starting from July 1, 2020, feed production enterprises will stop producing commercial feed containing growth-promoting drug feed additives (except Chinese herbal medicine). So far, the breeding industry has entered the era of antibiotic-free feed. However, the prohibition of antibiotics will lead to problems such as decreased animal production performance, increased disease rates, increased breeding costs, and reduced profitability in the livestock and poultry breeding process, which will cause relatively large economic losses and technical difficulties for domestic breeding enterprises. How to avoid the above-mentioned losses in the breeding environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163A23K20/189A23K50/30A23K50/60
CPCA23K10/18A23K20/163A23K20/189A23K50/30A23K50/60
Inventor 黄遵锡苗华彪韩楠玉唐湘华张呈波
Owner YUNNAN NORMAL UNIV
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