Method for detecting concentration of hydrocortisone in plasma by liquid chromatography-mass spectrometry

A technology of fludrocortisone and standard fludrocortisone, which is applied in the field of medicine and can solve the problems of speed, precision, sensitivity and selectivity that need to be improved

Inactive Publication Date: 2021-05-07
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speed, precision, sensitivity and selectivity of the existing methods for the determination of fludrocortisone need to be improved

Method used

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  • Method for detecting concentration of hydrocortisone in plasma by liquid chromatography-mass spectrometry
  • Method for detecting concentration of hydrocortisone in plasma by liquid chromatography-mass spectrometry
  • Method for detecting concentration of hydrocortisone in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Fludrocortisone Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Fludrocortisone (analyte): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0035] Fludrocortisone-d5 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0036] The reagents used are shown in Table 1 below:

[0037] Table 1 Reagent Details

[0038]

[0039]

[0040] Note: Reagents of the same grade or higher can also be used

[0041] The analytical equipment used is shown in Table 2 below:

[0042] Table 2 Details of equipment used

[0043] components model manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column oven AB SCIEX Autosampler (auto sampler) AC Autosampler AB SCIEX Sample rack Rack Changer AB SCIEX Mass spectrometer TRIPLE QUAD ...

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PUM

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Abstract

The invention discloses a method for detecting the concentration of hydrocortisone in plasma by liquid chromatography-mass spectrometry. The method adopts a liquid chromatography-mass spectrometry system for determination and comprises the following steps: firstly taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction, pretreating, separating by a chromatographic column, and detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for determining the blood concentration of the hydrocortisone; and the linear range of the plasma standard curve of the method is 0.01-3 ng/mL, the intra-batch and inter-batch precision RSD is less than +/-15%, and the method is suitable for measuring the concentration of the hydrocortisone in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for measuring medicines, in particular to a method for measuring fludrocortisone concentration in blood plasma by liquid mass spectrometry. Background technique [0002] Fludrocortisone acetate for partial replacement therapy in Addison's disease and salt-losing adrenal syndrome due to primary and secondary adrenal insufficiency. [0003] Fludrocortisone acetate mainly acts as a mineralocorticoid. Although it has certain glucocorticoid activity, the usual dose has no obvious glucocorticoid effect. Its physiological effect is similar to that of hydrocortisone, but the effect of fludrocortisone acetate on electrolyte balance and carbohydrate metabolism is significantly higher and longer than that of hydrocortisone. As a mineralocorticoid, fludrocortisone acetate can act on the distal tubules of the kidney to enhance the reabsorption of sodium ions from the tubular fluid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 范志鸿
Owner 徐州立兴佳正医药科技有限公司
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