Preparation method and application of norcantharidin-loaded exosome

Abstract

The invention belongs to the field of new dosage forms of pharmaceutical tumor drugs, and particularly relates to a preparation method and application of norcantharidin-loaded exosomes. The method specifically comprises the following steps of (1), extracting mesenchymal stem cell exosomes by adopting an ultracentrifugation method; and (2), wrapping norcantharidin in the exosomes through an electroporation method to obtain the drug-loaded exosomes. The prepared drug-loaded exosomes can improve the liver targeting property of the drug and enhance the drug effect of norcantharidin.

Description

technical field

[0001] The invention belongs to the field of new formulations of pharmaceutical tumor drugs, and in particular relates to a norcantharidin-loaded exosome and a preparation method thereof. Background technique

[0002] With the rapid development of nanotechnology, researchers have been committed to the development of new carriers that can significantly improve drug targeting, such as liposomes, nanoparticles, nanospheres, etc., these carriers take advantage of their small particle size (<1μm ), which can be taken up by organs rich in reticuloendothelial system, such as liver, spleen, bone marrow, etc., thus forming passive targeting. Or it can be structurally modified or transformed so that it has a special affinity for certain tissues and organs, so as to achieve active targeting effects. However, after a large number of animal and clinical trials, it has been found that nanocarriers also have many disadvantages: nanocarriers, as exogenous substances, may...

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