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Method for screening optimal proportion of calcium carbonate from fermentation liquor and application thereof

A technology of calcium carbonate and fermented liquid, which is applied in the direction of fermentation and chemical analysis by titration, which can solve the problems of high cost and long time consumption, and achieve the effects of increasing biological potency, improving the accuracy of addition, and reducing process and cost

Active Publication Date: 2021-05-11
安徽普洛生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, in order to determine the optimal addition amount of calcium carbonate in the fermented liquid, it is necessary to adopt an orthogonal experiment for optimization. Generally, the addition amount of calcium carbonate is changed to carry out a series of parallel experiments, and the fermentation ratio is determined according to the fermentation results. The whole The process is time-consuming and expensive, and the results are only applicable to this specific fermentation broth

Method used

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  • Method for screening optimal proportion of calcium carbonate from fermentation liquor and application thereof
  • Method for screening optimal proportion of calcium carbonate from fermentation liquor and application thereof
  • Method for screening optimal proportion of calcium carbonate from fermentation liquor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The experimental group and the control group in the kitasomycin fermentation medium are mainly different in the proportion of light calcium carbonate, which are 0.26% and 0.31% respectively. The ratio of other substances is:

[0040] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ powder 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 bubble enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-propanol 0.30 Soybean oil 2.4

[0041] The fermentation liquid at the end of the fermentation was tested for biological titer, and the test group and the control group were 10850u / ml and 10784u / ml respectively.

Embodiment 2

[0043] The experimental group and the control group in the kitastomycin fermentation medium are mainly different in the proportion of light calcium carbonate, which are 0.19% (about 1.36 times the actual utilization amount) and 0.31% respectively. The ratio of other substances is:

[0044] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ powder 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 bubble enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-propanol 0.30 Soybean oil 2.4

[0045] The fermentation broth at the end of the fermentation was tested for biological titer, and the test group and control group were 8159u / ml and 10893u / ml respectively.

[0046] The result of embodiment 2 shows that when the ratio of light calcium carbonate is controlled at 1.36 times of the actu...

Embodiment 3

[0048] The experimental group and the control group in the kitastomycin fermentation medium are mainly different in the proportion of light calcium carbonate, which are 0.21% (about 1.46 times of the actual utilization amount) and 0.31% respectively. The ratio of other substances is:

[0049] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ powder 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 bubble enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-propanol 0.30 Soybean oil 2.4

[0050]The fermentation liquid at the end of the fermentation was tested for biological titer, and the test group and the control group were 10865u / ml and 10776u / ml respectively.

[0051] The results of Example 3 show that when the proportion of light calcium carbonate is increased to 1.46 times of...

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Abstract

The invention discloses a screening method for screening an optimal proportion of calcium carbonate from fermentation liquor. The screening method comprises the following steps that the fermentation liquor at a production end point is filtered to obtain clean filtrate; an oxalic acid solution is prepared for titration of the filtrate, and a theoretical value of the required amount of oxalic acid completely removed from calcium carbonate added in the fermentation formula is calculated; different amounts of the oxalic acid are sequentially and progressively added according to theoretical values, an obtained solution is subjected to vacuum suction filtration again, the oxalic acid solution is added into the filtrate after suction filtration again, stood for at least 2 hours, and observed whether precipitates are generated or not; and the actual utilization amount of the calcium carbonate is calculated according to a precipitation generation condition, and then the optimal proportion of the calcium carbonate is determined according to the actual utilization amount. The invention also discloses improved kitasamycin fermentation liquor and tylosin fermentation liquor at the same time. According to the method, the proportion of the calcium carbonate is optimized according to the utilization condition of the calcium carbonate, so that the accuracy of the addition amount of the calcium carbonate is improved, the difficulty and cost of post-treatment of the fermentation liquor are reduced, and the biological value of the fermentation liquor is also improved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic drugs, and in particular relates to a method for screening the optimal ratio of calcium carbonate from fermentation broth and its application. Background technique [0002] The main role of calcium carbonate in the fermentation medium is as a pH buffer, which can neutralize some organic and inorganic acids to ensure the stability of the pH of the entire system. After neutralization, calcium will exist in the state of ions. In order to maintain the stability of pH in the fermentation process, excessive pH buffer agent calcium carbonate is often added in the proportioning of the fermentation medium. However, too much calcium ions will cause trouble for the post-treatment of the fermentation broth. At present, oxalic acid is often used to remove calcium ions in the pretreatment of the fermentation broth after fermentation. The excess calcium carbonate will react with oxalic acid during the pretrea...

Claims

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Application Information

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IPC IPC(8): C12P19/62G01N31/16
CPCC12P19/62G01N31/16
Inventor 纪春华江志诚许永兴胡露华钱丽丽金鑫强
Owner 安徽普洛生物科技有限公司
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