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A method for screening the optimal ratio of calcium carbonate from fermentation broth and its application

A technology of calcium carbonate and fermentation broth, applied in fermentation, chemical analysis by titration method, etc., can solve the problems of long time and high cost, and achieve the effect of improving biological titer, reducing process and cost, and improving adding accuracy.

Active Publication Date: 2022-05-27
安徽普洛生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, in order to determine the optimal addition amount of calcium carbonate in the fermented liquid, it is necessary to adopt an orthogonal experiment for optimization. Generally, the addition amount of calcium carbonate is changed to carry out a series of parallel experiments, and the fermentation ratio is determined according to the fermentation results. The whole The process is time-consuming and expensive, and the results are only applicable to this specific fermentation broth

Method used

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  • A method for screening the optimal ratio of calcium carbonate from fermentation broth and its application
  • A method for screening the optimal ratio of calcium carbonate from fermentation broth and its application
  • A method for screening the optimal ratio of calcium carbonate from fermentation broth and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The ratio of light calcium carbonate in the test group and the control group in the guitarmycin fermentation medium was different, which were 0.26% and 0.31%, respectively. The proportions of other substances are:

[0040] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ meal 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 Bubble Enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-Propanol 0.30 Soybean oil 2.4

[0041] The fermentation broth at the end of fermentation was tested for biological titer, the experimental group and the control group were 10850u / ml and 10784u / ml respectively.

Embodiment 2

[0043] The proportion of light calcium carbonate in the test group and the control group in the guitarmycin fermentation medium was different, which were 0.19% (about 1.36 times of the actual utilization) and 0.31%, respectively. The proportions of other substances are:

[0044] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ meal 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 Bubble Enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-Propanol 0.30 Soybean oil 2.4

[0045] The fermentation broth at the end of the fermentation was tested for biological titer, the experimental group and the control group were 8159u / ml and 10893u / ml, respectively.

[0046] The result of embodiment 2 shows that when the ratio of light calcium carbonate is controlled at 1.36 times of the actual u...

Embodiment 3

[0048] The ratio of light calcium carbonate in the test group and the control group in the guitarmycin fermentation medium was different, which were 0.21% (about 1.46 times of the actual utilization) and 0.31%, respectively. The proportions of other substances are:

[0049] raw material name Raw material ratio, % raw material name Raw material ratio, % corn starch 1 protein powder 0.21 wheat germ meal 1.15 methyl oleate 1.128 soybean meal 0.94 Potassium dihydrogen phosphate 0.073 H8 Bubble Enemy 0.019 Manganese chloride 0.052 glucose 1.50 Zinc sulfate 0.00625 n-Propanol 0.30 Soybean oil 2.4

[0050]The fermentation broth at the end of fermentation was tested for biological titer, the experimental group and the control group were 10865u / ml and 10776u / ml respectively.

[0051] The results of Example 3 show that when the proportion of light calcium carbonate is increased to 1.46 times the actual utilization a...

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Abstract

The invention discloses a screening method for screening the optimal ratio of calcium carbonate from fermentation broth, which comprises the following steps: obtaining a clean filtrate by filtering the fermentation broth at the production end; preparing oxalic acid solution for titration of the filtrate, and calculating the fermentation formula at the same time The theoretical value of the amount of oxalic acid required for the complete removal of the calcium carbonate added in the method; according to the theoretical value, different amounts of oxalic acid are added in descending order, and the resulting solution is vacuum filtered again, and the filtered filtrate is added to the oxalic acid solution again, and it is placed for at least 2 hours. Whether precipitation occurs; calculate the actual utilization of calcium carbonate according to the situation of precipitation, and then determine the optimal proportion of calcium carbonate according to the actual utilization. The invention also discloses the improved kitasomycin fermentation liquid and tylosin fermentation liquid at the same time. The present invention uses the utilization of calcium carbonate to optimize its ratio, which improves the accuracy of the amount of calcium carbonate added, reduces the difficulty and cost of post-treatment of the fermented liquid, and improves the biological potency of the fermented liquid.

Description

technical field [0001] The invention belongs to the technical field of antibiotic medicines, and in particular relates to a method for screening the optimum ratio of calcium carbonate from fermentation broth and use thereof. Background technique [0002] The main function of calcium carbonate in the fermentation medium is as a pH buffer, which can neutralize some organic acids and inorganic acids to ensure the stability of the pH of the entire system. After neutralization, calcium will exist in the state of ions. In order to maintain the pH stability during the fermentation process, excess pH buffer calcium carbonate is often added to the ratio of the fermentation medium. However, too much calcium ions will bring trouble to the post-processing of the fermentation broth. At present, oxalic acid is often used to remove calcium ions in the pretreatment of the fermentation broth after fermentation, and the excess calcium carbonate will react with oxalic acid during the pretreatm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62G01N31/16
CPCC12P19/62G01N31/16
Inventor 纪春华江志诚许永兴胡露华钱丽丽金鑫强
Owner 安徽普洛生物科技有限公司
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