HPLC-UV method for detecting concentration of levetiracetam in human plasma

Abstract

The invention discloses an HPLC-UV method for detecting the concentration of levetiracetam in human plasma. The method is characterized in that the concentration of levetiracetam in human plasma is detected by combining a methanol direct protein precipitation method with HPLC-UV, the concentration of levetiracetam can be detected only by using a very small amount of plasma, the linear range is 2-100 [mu]g/mL (R2 is more than 0.99), the intra-batch precision and the inter-batch precision are both smaller than or equal to 3.58%, and the accuracy range is 96.09-97.86%. According to the present invention, the detection limit is low, the selectivity is good, the specificity and the sensitivity are extremely good, the whole detection time is short, the residue, the interference and the extraction recovery rate meet the requirements, and the detection result is stable.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an HPLC-UV method for detecting the concentration of levetiracetam in human plasma. Background technique [0002] Levetiracetam is a new type of antiepileptic drug, which can be used as a monotherapy for partial seizures, or as an adjuvant treatment for partial, myoclonic and primary generalized tonic-clonic seizures. It was introduced into China in 2007. Its structure and antiepileptic mechanism are different from other drugs, and it mainly plays a role by selectively inhibiting the hypersynchrony of epileptiform burst discharge and the spread of epileptic seizures. The pharmacokinetics of levetiracetam differ between children and adults, and the pharmacokinetic processes of absorption, distribution, metabolism, and excretion change with growth and development. Although levetiracetam is easy to administer and tolerated, drug monitoring is required in many clinical settings (su...

AI Technical Summary

Problems solved by technology

Although levetiracetam is easy to administer and tolerated, drug monitoring is required in many clinical settings (suspected non-compliance, pregnancy, pediatric dosing, renal failure, and high-dose futility)

At present, HPLC or LC-MS / MS are recommended for the determination of levetiracetam. The sample pretreatment of LC-MS / MS is complicated and time-consuming, and the equipment is expensive, so it is difficult to be widely used in daily clinical practice.

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