Novel coronavirus neutralizing antibody detection test paper

A coronavirus and antibody detection technology, applied in the field of biomedical detection, can solve problems such as time-consuming, low detection specificity, and complicated detection operations

Pending Publication Date: 2021-05-14
NANTONG EGENS BIOTECH
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is that the existing novel coronavirus neutralizing antibody detection operation is relatively complicated and time-consuming, and the detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel coronavirus neutralizing antibody detection test paper
  • Novel coronavirus neutralizing antibody detection test paper
  • Novel coronavirus neutralizing antibody detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Novel coronavirus neutralizing antibody detection test paper

[0058] This embodiment provides a novel coronavirus neutralizing antibody test strip, including:

[0059] Such as figure 1 As shown, it includes a substrate 1 and a sample pad 2, a colloidal gold adsorption pad 3, an antibody carrying film 4 and a water absorbing pad 5 are overlapped and overlapped on the substrate 1 in sequence; the antibody carrying film 4 is provided with a corresponding The detection line T1 , the detection line T2 and the quality control line C are spaced apart, the detection line T2 is close to the colloidal gold adsorption pad 3 , and the quality control line C is close to the water absorption pad 5 . The colloidal gold adsorption 3 is coated with the receptor binding domain RBD antigen of the new coronavirus S protein labeled with colloidal gold and the antibody unrelated to the new coronavirus labeled with colloidal gold. In this embodiment, the antibody unrelated to the ...

Embodiment 2

[0062] This embodiment provides a method for preparing the novel coronavirus neutralizing antibody detection test paper in Example 1, and the preparation steps of the test paper are as follows:

[0063] 1. Preparation of antibody-carrying membrane:

[0064] (1) Select a nitrocellulose membrane with a pore size of 3 μm to 10 μm, and cut the membrane into specifications with a width of 2.0 cm and a length of 30.5 cm as required, for later use.

[0065] (2) Use 0.1M, pH8.0 Tris-HCL buffer solution to prepare the mouse anti-human IgG monoclonal antibody used for the detection line coating. ml, the concentration of ACE-2 is 1.5 to 2.0 mg / ml, in this example it is 1.8 mg / ml, and the goat anti-rabbit IgG used for quality control line coating is prepared with 0.85% by mass sodium chloride buffer For polyclonal antibodies, the antibody concentration is 2.0-2.5 mg / ml, which is 2.2 mg / ml in this embodiment.

[0066] (3) Select the antibody-coated surface of the nitrocellulose membrane ...

Embodiment 3

[0080] This embodiment provides a novel coronavirus neutralizing antibody detection device, such as figure 2 As shown, a housing 7 is included, and two novel coronavirus neutralizing antibody detection test papers of embodiment 1 are arranged side by side inside the housing 7 . Along the chromatographic direction of the novel coronavirus neutralizing antibody detection test paper on the housing 7, a sample hole 8 is set at the corresponding sample pad, and an observation port 9 is set at the detection line T2, the detection line T1 and the quality control line C .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Widthaaaaaaaaaa
Lengthaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of biomedical detection and particularly relates to novel coronavirus neutralizing antibody detection test paper, a kit, a detection method and application. The novel coronavirus neutralizing antibody detection test paper comprises a substrate, and a sample loading pad, a colloidal gold adsorption pad, an antibody bearing film and a water absorption pad which are overlapped on the substrate in sequence; wherein a detection line T1, a detection line T2 and a quality control line C are arranged on the antibody bearing film at intervals, the detection line T2 is close to the colloidal gold adsorption pad, and the quality control line C is close to the water absorption pad; the colloidal gold absorption pad is coated with a colloidal gold labeled novel coronavirus S-RBD antigen and a colloidal gold labeled antibody irrelevant to a new coronavirus, and the detection line T1 is coated with a second antibody of a new coronavirus antibody IgG; the detection line T2 is coated with angiotensin converting enzyme 2; the quality control line C is coated with a secondary antibody which is specifically combined with an antibody irrelevant to the colloidal gold labeled new coronavirus; the test paper can be used for detecting the novel coronavirus neutralizing antibody, and is simple to operate, short in time, and high in specificity and sensitivity during detection.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a novel coronavirus neutralizing antibody detection test paper, kit, detection method and application. Background technique [0002] As the global fight against the novel coronavirus epidemic enters a protracted stage, in addition to the new crown vaccine that is still in the clinical trial stage, neutralizing antibodies that can effectively block the new crown virus have also been discovered one after another. As a candidate therapy, it has brought new ideas to the academic world. hope. [0003] Antibody is a protein with immune effect produced by B lymphocytes in the human body stimulated by antigens, and mainly exists in blood and tissues. Antibodies have a Y-shaped structure, which helps them precisely capture antigens. After microorganisms invade the human body, the body is stimulated to produce a variety of antibodies, only some of which can recognize the microorganism...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/569G01N33/558G01N33/543G01N33/531
CPCG01N33/6857G01N33/56983G01N33/543G01N33/558G01N33/531G01N2333/165
Inventor 欧卫军孙一品顾飞褚晖
Owner NANTONG EGENS BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap