Nano antibody for resisting glycosyl transferase A subunit and application of nano antibody

A technology of glycosyltransferase and nanobody, applied in the direction of antibody, application, anti-enzyme immunoglobulin, etc.

Active Publication Date: 2021-05-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no reports of specific antibodies against glycosyltransferase A RBD

Method used

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  • Nano antibody for resisting glycosyl transferase A subunit and application of nano antibody
  • Nano antibody for resisting glycosyl transferase A subunit and application of nano antibody
  • Nano antibody for resisting glycosyl transferase A subunit and application of nano antibody

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Embodiment

[0021] There is an antibody naturally lacking light chain in the serum of alpaca, that is, heavy-chain antibody (hcAb). Single domain heavy chain antibody (single domain antibody, sdAb) refers to a genetically engineered antibody composed only of the heavy chain antibody variable region (Variable region), also known as VHH antibody (variable domain of heavychain of heavy-chain antibody, VHH antibody ) or nanobody (Nanobody, Nb). Compared with traditional antibodies, single-domain heavy chain antibodies have a small molecular weight, strong stress resistance, and high activity under strong acid conditions, so this is very suitable for delivery into the intestinal tract through the stomach, while maintaining good activity involved in the inhibition of the difficile shuttle bacteria. The present invention uses phage display technology to screen out phage-positive clones capable of binding to the target molecule glycosyltransferase A subunit from an alpaca phage library, thereby ...

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Abstract

The invention discloses a nano antibody for resisting glycosyl transferase A subunit and application of the nano antibody. A variable region of the nano antibody is provided with three complementary determining regions, namely CDR-1, CDR-2 and CDR-3, the CDR-1 is composed of an amino acid sequence as shown in SEQ ID NO.1, the CDR-2 is composed of an amino acid sequence as shown in SEQ ID NO.2, and the CDR-3 is composed of an amino acid sequence as shown in SEQ ID NO.3. The nano antibody for resisting the glycosyl transferase A subunit has the activity of being specifically bonded to the glycosyl transferase A subunit, neutralizes the activity of the glycosyl transferase A, can be used for detecting clostridium difficile and can also be used for preparing drugs for treating and resisting the clostridium difficile; the nano antibody also has the characteristics of small molecular weight, high affinity, stable structure and performance and the like; and the nano antibody has the advantages of being capable of tolerating the gastric acid environment and being not easily degraded by pepsin and the like.

Description

technical field [0001] The invention belongs to the technical field of antibodies and relates to nanobody technology, in particular to a nanobody against glycosyltransferase A (TcdA) subunit and its application. Background technique [0002] Clostridium difficile (CD) is widely distributed in the natural environment and can survive in water, soil, vegetables, dogs, cats and hospital environments, and is a normal flora in the intestinal tract of animals and humans. Irregular use of antibiotics will cause intestinal flora imbalance, and drug-resistant CD will grow and multiply in large numbers, leading to diseases such as antibiotic-associated diarrhea and pseudomembranous enteritis, which are the main pathogens of antibiotic-associated diarrheal diseases. The spores of CD have strong resistance and are difficult to eliminate. They can exist in the hospital environment for a long time, making CD one of the main pathogenic bacteria of hospital-acquired diarrhea. [0003] The i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/40C07K16/12C12N15/13A61K39/40A61P31/04G01N33/68G01N33/569
CPCC07K16/40C07K16/1282G01N33/6854G01N33/56911C07K2317/569C07K2317/565C07K2317/56C07K2317/76C07K2317/94C07K2317/92G01N2333/33G01N2469/10A61P31/04A61K2039/505Y02A50/30
Inventor 郭刚刘宇刘开云舒月力李彦王宁
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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