Symphytum officinale polysaccharide based on response surface method optimized enzyme-assisted extraction and preparation method and application thereof

A response surface methodology, a technology of polymerizing grass, applied in the field of polymerized grass polysaccharide and its preparation, can solve the problems that the research on the effect of polymerized grass polysaccharide enzyme on chicken cecal flora has not been carried out, and the research of polymerized grass polysaccharide is not in-depth enough.

Inactive Publication Date: 2021-05-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on polysaccharides is not deep enough
Only a few scholars have analyzed the hot water extraction, in vitro antioxidant and hypoglycemic activities of polysac

Method used

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  • Symphytum officinale polysaccharide based on response surface method optimized enzyme-assisted extraction and preparation method and application thereof
  • Symphytum officinale polysaccharide based on response surface method optimized enzyme-assisted extraction and preparation method and application thereof
  • Symphytum officinale polysaccharide based on response surface method optimized enzyme-assisted extraction and preparation method and application thereof

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preparation example Construction

[0045]First, the present invention provides a method for preparing a polymerized polysaccharide with a response surface method optimizing an enzyme, including the following steps:

[0046]Step 1: Extract

[0047]The polymer wash was washed and pulverized, and the drying temperature of the polymerization was preferably from 50 ° C, and the sieve size was preferably 1 mM to obtain polymerization grass powder; the resulting polymerized flour powder was soaked. At the same time, the complex enzyme was added and the pH was adjusted to obtain an extract, and the polymerization powder soaking temperature is preferably 50 ° C, and the polymerization powder soaking time is preferably 60 min, and the amount ratio of the water and polymerization grass powder is preferably 20mL / g, the composite enzyme as a hydraulic condition having a hydrated amount of 1%, the specific component and mass ratio of the composite enzyme: pectinase: papain protease = 1: 1: 1, the adjustment pH value Preferably, the ex...

Embodiment 1

[0065]The polyheetic washed, dried under 50 ° C, and pulverized it was dried and passed by 1 mm sieve to obtain a polymerization powder; add the obtained polymeral powder to water to soak for 60 min, and 1% compound enzyme The pH is adjusted to obtain the extract, the amount of water and polymerization powder is 20 mL / g, the composite enzyme specific component and mass ratio of cellulase: pectinase: papain protease = 1: 1 : 1; After soaking, the extract is filtered, the filtrate is concentrated to concentrate;

[0066]Step 2: Alcoholization

[0067]The concentration of 75% in the concentration of 75% was slowly added to the concentrate, and placed at 4 ° C for 12 h, then centrifuged, centrifugal speed was 3000 r / min, centrifugal time was 15 min, and the supernatant was discarded after centrifugation. Polymerized polysaccharide precipitation;

[0068]Step 3: Go to the protein

[0069]The resulting polymerized grass polysaccharide precipitate was dissolved in water, and the polymerized polysa...

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Abstract

The invention discloses symphytum officinale polysaccharide based on response surface method optimized enzyme-assisted extraction and a preparation method and application thereof, and belongs to the technical field of raw material deep processing technologies. The preparation method of the symphytum officinale polysaccharide comprises the following steps: step 1, extracting; step 2, alcohol precipitation and impurity removal; step 3, deproteinizing; step 4, dialyzing and desalting; step 5, carrying out DEAE-52 cellulose column chromatography; step 6, carrying out Sepharose CL-6B agarose gel column chromatography. the invention also provides an application of the symphytum officinale polysaccharide in regulating and controlling the abundance of probiotics in chicken intestinal tracts, and a complete and feasible technical route for extracting, separating and purifying the symphytum officinale polysaccharide and researching physicochemical properties and biological activity of the symphytum officinale polysaccharide is established.

Description

Technical field[0001]The present invention relates to the field of deep processing of plant materials, and more particularly to a polymerized polysaccharide based on a response surface method optimized enzyme and its preparation method and application.Background technique[0002]Intestinal health refers to a variety of physiological activities, intestinal flora and various physiological functions to maintain the intestinal environmental stability. Intestinal health is closely related to animal production performance, and intestinal health and animal health are synonymous. In sustained intestinal health, the intestinal flora is an animal metabolic reaction, an immune system, and a healthy regulator play a key role in animal health and production. Good intestinal flora is critical to the best growth performance of poultry, and unfavorable intestinal flora may promote intestinal infection, resulting in a decline in growth and increase in mortality.[0003]In recent years, natural growth ac...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/14A61P1/00
CPCC08B37/0003A61P1/14A61P1/00
Inventor 尚红梅张南翼郭洋张鹤翔曹梓航刘梦雪
Owner JILIN AGRICULTURAL UNIV
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