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A strain of Brevibacterium halophilus capable of efficiently degrading ammonia nitrogen and its application

A technology for degrading ammonia nitrogen and brevibacterium, applied in bacteria, microbial-based methods, biological water/sewage treatment, etc., to achieve the effects of short treatment time, good tolerance, and strong ammonia nitrogen degradation ability

Active Publication Date: 2022-05-03
浙江幸双环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report that Brevibacterium flaxensis has the ability to tolerate salt and degrade ammonia nitrogen efficiently

Method used

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  • A strain of Brevibacterium halophilus capable of efficiently degrading ammonia nitrogen and its application
  • A strain of Brevibacterium halophilus capable of efficiently degrading ammonia nitrogen and its application
  • A strain of Brevibacterium halophilus capable of efficiently degrading ammonia nitrogen and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: screening isolated bacterial strains

[0029] Selective solid medium: tryptone 5.00g / L; yeast extract 1.00g / L; sodium chloride 19.45g / L; anhydrous MgCl5.90g / L; 2 SO 4 10H 2 O 3.24g / L; CaCl 1.80g / L; KCl 0.55g / L; NaHCO 3 0.16g / L;H 3 BO 3 22mg / L; Na 2 HPO 4 8mg / L; agar 15g / L.

[0030] The strains were selected from the high-salt sewage of a factory in Lishui. Take 50mL of sewage, centrifuge at 12000rpm for 10min, discard the supernatant, add water to 1mL and mix well. Add 9 mL of water to form 10 -1 diluent. from 10 -1 Add 1mL of the diluted solution to 9mL sterile water to make 10 -2 Dilution, and so on, make a series of gradient dilutions on the finally obtained bacterial solution, and take 0.1mL to spread on the separation medium, and incubate at a constant temperature of 30°C until a single colony grows. Separation and purification of single colonies with different colors and shapes were carried out, and 6 single colonies were picked. After...

Embodiment 2

[0031] Example 2: Ammonia nitrogen degradation ability test of bacterial strains

[0032] 1 Ammonia nitrogen standard curve

[0033] In eight 50mL volumetric flasks, add 0.00mL, 0.50mL, 1.00mL, 2.00mL, 4.00mL, 8.00mL, and 10.00mL of ammonia nitrogen standard working solution, and the corresponding ammonia nitrogen contents are 0.0μg, 5.0μg, 10.0μg, 20.0μg, 40.0μg, 60.0μg, 80.0μg and 100μg, add water to the marked line. Add 1.0mL potassium sodium tartrate solution, shake well, then add 1.0mL Nessler's reagent, shake well. After standing for 10 minutes, at a wavelength of 420nm, use a 10mm cuvette and use water as a reference to measure the absorbance. The absorbance after blank correction is taken as the ordinate, and the corresponding ammonia nitrogen content (μg) is used as the abscissa to draw a calibration curve. The obtained standard curve is as figure 2 shown.

[0034] Ammonia nitrogen degradation ability of 2 strains

[0035] Pick a single colony from the plate and...

Embodiment 3

[0051] Embodiment 3: No. 1, No. 6 bacterial strains salt tolerance test

[0052] 1 Growth curves under different salinity

[0053] The growth curves of No. 1 bacterial strain and No. 6 bacterial strain were detected respectively under 1%, 4%, 7%, and 10% salinity, and the results were as follows: Figure 4 shown. From Figure 4 It can be seen that both strain No. 1 and strain No. 6 can grow and reproduce at a salinity of 1%-10%.

[0054] 2 Ammonia nitrogen degradation capacity under different salinity

[0055] Inoculate No. 1 and No. 6 strains into liquid LB medium, and culture them on a shaker at 220 rpm at 30°C for 1 day. Take 2 mL of the bacterial solution and centrifuge at 12,000 rpm for 1 min, add 1 mL of deionized water to resuspend, centrifuge at 12,000 rpm for 1 min, discard the supernatant, and repeat for 4 groups. The sediment was resuspended with artificial water samples with salinity of 1%, 4%, 7% and 10%, respectively, and then 100 mL of corresponding artific...

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Abstract

The invention relates to the field of sewage treatment, and discloses a halophilic Brevibacterium flaxenum capable of efficiently degrading ammonia nitrogen and an application thereof. The Brevibacterium flaxenum is named B1, and it has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on July 2, 2019. Its preservation number is CGMCC NO.18298, and the microbial classification is named Brevibacterium flaxenum Brevibacterium lines . The Brevibacterium flaxenum B1 has a strong ability to degrade ammonia nitrogen and can tolerate high-salt environments. It can grow and maintain a high removal efficiency of ammonia nitrogen under the condition of 1%-10% salinity, and can be applied to ammonia nitrogen in high-salt sewage Degradation treatment, and has the advantage of short treatment time.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a strain of Brevibacterium halophilus capable of efficiently degrading ammonia nitrogen and its application. Background technique [0002] Chemical, food, leather, aquaculture and other industries generate large amounts of high-salinity wastewater, accounting for about 5% of global wastewater discharge. Hypersaline wastewater is usually rich in high concentrations of nitrogen, soluble inorganic salts, and organic compounds. Due to the important part of high-salt wastewater in sewage treatment and marine environmental protection, the treatment of high-salt wastewater has aroused widespread concern in society. [0003] In wastewater denitrification, physicochemical methods are costly and may produce other harmful secondary pollutants. Compared with physical and chemical methods, biological methods have good treatment performance, relatively low energy consumption and low investmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/13C02F101/16
CPCC12N1/20C02F3/34C02F2101/16
Inventor 吴杰群沈巧娜
Owner 浙江幸双环保科技有限公司
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