A kind of microbial denitrification method of high-salt sewage

A high-salt sewage and microbial technology, applied in the field of microbial denitrification of high-salt sewage, to achieve strong ammonia nitrogen degradation ability, improve ammonia nitrogen degradation efficiency, and high denitrification efficiency

Active Publication Date: 2022-07-01
浙江可龙环保科技有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report that Brevibacterium flaxensis has the ability to tolerate salt and degrade ammonia nitrogen efficiently

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of microbial denitrification method of high-salt sewage
  • A kind of microbial denitrification method of high-salt sewage
  • A kind of microbial denitrification method of high-salt sewage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Screening of isolated strains

[0055] Selective solid medium: tryptone 5.00g / L; yeast extract 1.00g / L; sodium chloride 19.45g / L; anhydrous MgCl 5.90g / L; Na 2 SO 4 ·10H 2 O 3.24g / L; CaCl 1.80g / L; KCl 0.55g / L; NaHCO 3 0.16g / L; H 3 BO 3 22mg / L; Na 2 HPO 4 8mg / L; agar 15g / L.

[0056] The strains were screened from high-salt sewage from a factory in Lishui, 50 mL of sewage was taken, centrifuged at 12,000 rpm for 10 min, the supernatant was discarded, and water was added to 1 mL and mixed. Add 9 mL of water to form 10 -1 diluent. from 10 -1 Take 1 mL of the dilution solution and add 9 mL of sterile water to make 10 -2 Diluent, and so on, make a series of gradient dilutions on the finally obtained bacterial liquid, and take 0.1 mL of it and spread it on the separation medium, and cultivate at 30°C until a single colony grows. The single colonies with different colors and shapes were streaked and purified, and 6 single colonies were picked. After pur...

Embodiment 2

[0057] Example 2: Ammonia nitrogen degradation ability test of strain

[0058] 1 Ammonia nitrogen standard curve

[0059] In eight 50mL volumetric flasks, add 0.00mL, 0.50mL, 1.00mL, 2.00mL, 4.00mL, 8.00mL, and 10.00mL ammonia nitrogen standard working solutions respectively, and the corresponding ammonia nitrogen contents are 0.0μg, 5.0μg, 10.0 μg, 20.0 μg, 40.0 μg, 60.0 μg, 80.0 μg and 100 μg, add water to the mark. Add 1.0mL potassium sodium tartrate solution, shake well, then add 1.0mL Nessler reagent, shake well. After standing for 10min, at a wavelength of 420nm, use a 10mm cuvette with water as a reference, measure the absorbance, take the absorbance after blank correction as the ordinate, and its corresponding ammonia nitrogen content (μg) as the abscissa, draw a calibration curve, The standard curve obtained as figure 2 shown.

[0060] Ammonia nitrogen degradation ability of 2 strains

[0061] A single colony was picked from the plate and inoculated into a liqui...

Embodiment 3

[0077] Example 3: Salt tolerance test of strains 1 and 6

[0078] 1 Growth curves at different salinities

[0079] The growth curves of strain 1 and strain 6 were detected under salinity of 1%, 4%, 7%, and 10%, respectively. The results are as follows Figure 4 shown. from Figure 4 It can be seen that both strains No. 1 and No. 6 can grow and reproduce under the salinity of 1%-10%.

[0080] 2. Ammonia nitrogen degradation ability under different salinity

[0081] Strain No. 1 and No. 6 were inoculated into liquid LB medium, and cultured at 30° C. shaker at 220 rpm for 1 day. Take 2mL of bacterial solution and centrifuge at 12000rpm for 1min, add 1mL of deionized water to resuspend, centrifuge at 12000rpm for 1min to discard the supernatant, and repeat for 4 groups. The precipitates were resuspended with artificial water samples with salinities of 1%, 4%, 7% and 10%, respectively, and then put into 100 mL of corresponding artificial water samples, and incubated at 30°C wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of sewage treatment, and discloses a microbial denitrification method for high-salt sewage, comprising the following steps: (1) adding sewage into a conditioning tank, adding a carbon source and Brevibacterium linax inoculum into the conditioning tank, and mixing evenly; The Brevibacterium flax is named B1, and the preservation number is CGMCC NO.18298; (2) The treated sewage is passed into the coagulation sedimentation tank, and ferrous salt, alkali and flocculant are added to the coagulation sedimentation tank, and the mixture is uniform. (3) Pass the supernatant obtained after coagulation and sedimentation treatment into the denitrification tank for denitrification, and then pass it into the nitrification tank for nitrification; (4) Pass the nitrified sewage into the ultrafiltration system for ultrafiltration. Filtration, and the filtrate was treated with nanofiltration / reverse osmosis membrane. The Brevibacterium flax B1 used in the present invention has strong ammonia nitrogen degradation ability and good tolerance to high-salt environment. After combining it with a membrane bioreactor, it has high denitrification efficiency for high-salt sewage.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a microbial denitrification method for high-salt sewage. Background technique [0002] Chemical, food, leather, aquaculture and other industries generate large volumes of high salinity wastewater, accounting for about 5% of global wastewater discharge. High salinity wastewater is often rich in high concentrations of nitrogen, soluble inorganic salts and organic compounds. Due to the important part of high-salt wastewater in sewage treatment and marine environmental protection, the treatment of high-salt wastewater has attracted widespread attention in society. [0003] In wastewater denitrification, physicochemical methods are expensive and may generate other harmful secondary pollutants. Compared with physical and chemical methods, biological methods have good processing performance, relatively low energy consumption and lower investment / operating costs. However, when applied ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/14C02F3/34C02F1/52C02F1/44C02F101/16
CPCC02F9/00C02F3/34C02F1/52C02F1/5236C02F1/444C02F2101/16
Inventor 吴杰群沈巧娜
Owner 浙江可龙环保科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap