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Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof

A technology of cinnamaldehyde and ochrax, which is applied in the direction of botany equipment and methods, plant growth regulators, biocides, etc., can solve the problem of reducing the control effect and duration of cinnamaldehyde, inability to exert biological activity, and cinnamaldehyde is volatile, etc. problem, to achieve the effect of high coalescence stability, strong protection ability and good biocompatibility

Inactive Publication Date: 2021-06-01
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to reasons such as cinnamaldehyde is volatile and oxidized, the retention time in the environment is too short to exert its biological activity, which will reduce the control effect and duration of cinnamaldehyde to a large extent.

Method used

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  • Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof
  • Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof
  • Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation of cinnamaldehyde-containing emulsion

[0043] (1) Weigh 0.1g of chitosan, put it in a container, add 100ml of 1% (v / v) acetic acid aqueous solution, stir with a magnetic stirrer at 1000r / min for 12h until transparent, to obtain a chitosan solution;

[0044] (2) take by weighing the zein (MacLean, Z822847) of 1g, place in the second container, add 50ml of 80% (v / v) ethanol aqueous solution, stir 2h with magnetic stirrer at 1000r / min to obtain zein solution;

[0045] (3) take the chitosan solution obtained in step (1), place it in a third container, add the zein solution obtained in step (2), and use a probe-type ultrasonic extractor (20mm titanium alloy probe, 1200w power) to ultrasonicate for 5min, A mixed liquid containing chitosan and corn gluten composite particles is obtained;

[0046](4) Get the mixed liquid obtained in step (3) and place it in a round-bottomed flask, concentrate it with a rotary evaporator (the temperature of the water bat...

Embodiment 2

[0049] Embodiment 2: cinnamaldehyde antibacterial effect measurement

[0050] PDA medium: take 200g of peeled potatoes and boil for 30min, take the filtrate, add 20g of glucose and 16g of agar, dilute to 1000mL with deionized water, autoclave at 121°C for 15 minutes, cool down to about 55°C, add 7.5, 15, 30μL The cinnamaldehyde-containing emulsions prepared in the examples were mixed evenly with 20 ml of culture medium respectively, and poured onto plates. No cinnamaldehyde-containing emulsions were added to the control group, and three parallels were made for each.

[0051] Activation of strains: Inoculate Ochrae (CGMCC No.3.4412) on PDA medium, grow at 28°C for 7 days, and store at 4°C.

[0052] Ochra spore liquid preparation: Wash the ochra spores on the plate medium with sterile water and dissolve them in 0.01% Tween 80, dilute the initial spore liquid with sterile water to a spore concentration of 1×10 6 cfu / mL.

[0053] 10 μL (1×10 6 cfu / mL) inoculated in the center o...

Embodiment 3

[0055] Embodiment 3: Determination of antibacterial effect of cinnamaldehyde fumigation

[0056] PDA medium: take 200g of peeled potatoes and boil for 30min, take the filtrate, add 20g of glucose and 16g of agar, dilute to 1000mL with deionized water, autoclave at 121°C for 15 minutes, cool down to about 55°C and pour on plates, 20mL per plate.

[0057] Activation of strains: Inoculate Ochrae (CGMCC No.3.4412) on PDA medium, grow at 28°C for 7 days, and store at 4°C.

[0058] Ochra spore liquid preparation: Wash the ochra spores on the plate medium with sterile water and dissolve them in 0.01% Tween 80, dilute the initial spore liquid with sterile water to a spore concentration of 1×10 6 cfu / mL.

[0059] 10 μL (1×10 6 cfu / mL) inoculated in the center of PDA medium until completely absorbed. Place 3 pieces of sterilized filter paper with a diameter of 8 mm symmetrically on the edge of the petri dish cover, and put 30 μL of the emulsion containing cinnamaldehyde and the emuls...

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Abstract

The invention discloses cinnamaldehyde-containing emulsion for inhibiting the growth of aspergillus ochraceus and a preparation method thereof, the emulsion system is prepared from cinnamaldehyde, an emulsifier and water, the emulsifier is composite particles prepared from chitosan and corn protein through an ultrasonic method, the content of cinnamaldehyde in the emulsion is 5-30g / L, the content of chitosan is 0.5-2.5g / L, and the content of corn protein is 5-25g / L. Cinnamaldehyde biological resources are widely sourced in China, the production process is simple, the cost is low, and the cinnamaldehyde has volatility; cinnamaldehyde is good in safety to people and livestock and is listed in a food additive catalog (GB 2760-2014); the method is novel in thought, the emulsion preparation method is simple, and the prepared cinnamaldehyde-containing emulsion can keep good stability within 30 days and is high in practicability; the prepared cinnamaldehyde-containing emulsion has a good effect of inhibiting aspergillus ochraceus, and is safe and free of toxic and side effects.

Description

[0001] (1) Technical field [0002] The invention relates to a cinnamaldehyde-containing emulsion for inhibiting the growth of Aspergillus ochra and a preparation method thereof, belonging to the technical field of emulsion embedding and antibacterial. [0003] (2) Background technology [0004] Cinnamaldehyde (English: Cinnamaldehyde) is an aldehyde organic compound, which is a yellow viscous liquid, which exists in large quantities in plants such as cinnamon. The naturally occurring cinnamaldehydes in nature are all trans-structures, and the molecule is a phenyl group attached to acrolein, so it can be considered as an acrolein derivative. Cinnamaldehyde is a permitted synthetic flavor for food specified in GB2076-2014. It can be used to prepare flavors for meat, condiments, oral care products, chewing gum and candy. It can also be used as a preservative for surface-treated fresh fruits. Preserved preservation. The molecular formula is: C 9 H 8 O, molecular weight: 132.16...

Claims

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Application Information

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IPC IPC(8): A01N35/02A01N43/16A01N65/44A01P3/00A01P1/00
CPCA01N35/02A01N43/16A01N65/44
Inventor 王龑孙培龙林威能静蔡铭杨开
Owner ZHEJIANG UNIV OF TECH
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