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Composition for treating liver cancer and application thereof

A composition and liver cancer technology, applied in the field of biomedicine, can solve problems such as poor prognosis, achieve the effect of less toxic and side effects, and inhibit tumor growth

Active Publication Date: 2021-06-01
CANCER CENT OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, dox resistance often occurs in advanced tumors, leading to poor prognosis (Chen et al., 2018)

Method used

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  • Composition for treating liver cancer and application thereof
  • Composition for treating liver cancer and application thereof
  • Composition for treating liver cancer and application thereof

Examples

Experimental program
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Effect test

experiment example 1

[0041] 1. Cell culture, passage and cryopreservation

[0042] (1) Cell culture-related reagent preparation and cell culture:

[0043] 1X PBS preparation (1L): weigh 8g NaCl, 0.2g KCl, 3.58g Na 2 HPO 4 12H 2 o 2 ,0.24g KH 2 PO 4 , Dissolve in 800ml deionized water, stir evenly, and use a graduated cylinder to make up to 1L. Use after autoclaving.

[0044] 0.25% trypsin preparation (0.02% EDTA) (1L): add 8g NaCl, 0.4g KCl, 0.12g NaCl to about 800ml ultrapure water 2 HPO 4 12H 2 o 2 ,0.06g KH 2 PO 4 ,0.35g NaHCO 3 , 1g glucose, 0.01g phenol red, 2.5g trypsin, then add 1.07ml 0.5mol / L EDTA, stir well to dissolve, dilute to 1L, adjust pH to 7.2.

[0045] DMEM basic culture preparation (1L): Dissolve a pack of DMEM powder (Gibco, 12100-046) in about 800mL ultrapure water, stir thoroughly with a magnetic stirrer for 2 hours, weigh 3g HEPES, 3.17g NaHCO 3 Add it, wait for complete dissolution, and dilute to 1L.

[0046] The cells used in this study were all cultured in...

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Abstract

The invention belongs to the field of biological medicines, and particularly discloses a method for synergistically inducing cancer cell death through combination of Crizo and Dox. Compared with single-drug treatment, cell death is increased by at least 50% through combination of the two drugs, and the induction effect on liver cancer cell apoptosis is much higher than that of combination of first-line treatment drugs sorafenib and Dox for advanced liver cancer. The invention clarifies the mechanism of the action from the cellular level, and reveals that Crizo and Dox are combined to overcome the chemotherapeutic drug resistance by inhibiting the expression of MDR1 and inducing autophagic cell death. In-vivo experiments show that the combination of Crizo and Dox can effectively inhibit tumor growth on an animal model, has small toxic and side effects, and provides a potential treatment strategy for treating cancers, especially advanced liver cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, to a composition for treating liver cancer, specifically a pharmaceutical composition containing crizotinib and doxorubicin and its application in the preparation of drugs for treating liver cancer. Background technique [0002] Liver cancer is the sixth most common cancer worldwide and the third leading cause of cancer-related death. According to risk factors for liver cancer, the incidence and mortality of liver cancer were significantly related to age, sex, and geographic region. Primary liver cancer includes hepatocellular carcinoma (hepatocellular carcinoma, HCC), intrahepatic ductal carcinoma and other rare types. Among them, hepatocellular carcinoma with the highest degree of malignancy accounts for more than 80% and is the main cause of death in patients with liver cancer. Surgical resection and liver transplantation are the most effective means of treatment fo...

Claims

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Application Information

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IPC IPC(8): A61K31/4545A61K31/704A61P1/16A61P35/00
CPCA61K31/4545A61K31/704A61P1/16A61P35/00A61K2300/00
Inventor 李朝阳张必翔
Owner CANCER CENT OF GUANGZHOU MEDICAL UNIV
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