Culture and immunofluorescence quantitative detection method of human group H rotavirus
A quantitative detection method, immunofluorescence technology, applied in the field of human H group rotavirus culture and immunofluorescence quantitative detection, can solve the problem of quantification of non-viable virus
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[0080] 1. Experimental method
[0081]1.1 Culture MA104 cells
[0082] 1.1.2 Recovery of cells
[0083] (1) Take out the required cell cryopreservation tube from the liquid nitrogen tank, put it into warm water at 37°C and shake it back and forth to melt it quickly, and centrifuge at 800rpm / min for 3min.
[0084] (2) Pour off the cell freezing solution.
[0085] (3) Prepare cell culture medium containing 10% FBS, that is, 1mL FBS+9mL1640, and the actual dosage is calculated according to the specific amount required for each experiment.
[0086] (4) Draw 6mL of cell culture solution into a 25cm 2 of cell flasks.
[0087] (5) at 25cm 2 Pipette 1mL of cell culture medium into the cell flask and blow the cells gently. After blowing, suck the cell suspension back to 25cm 2 Place the cell flask in a carbon dioxide incubator.
[0088] (6) Carbon dioxide culture conditions: the temperature is 37° C., and the carbon dioxide content is 5%.
[0089] (7) Change the medium for the ...
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