Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method and application of flavonoid compound saponin c in Chinese saponins

A technology for flavonoids and compounds in thorn, which is applied in the field of preparation of the flavonoid acacia new ketone C in thorn thorn, can solve the problems of no anti-septic acute kidney injury drug and the like, and achieves high purity and sample recovery rate. High, low solvent consumption effect

Active Publication Date: 2022-05-31
HENAN UNIV OF CHINESE MEDICINE
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on chemical constituents have shown that saponins mainly contain flavonoids, phenolic acids, and terpenoids. Flavonoids are the main active ingredients in saponins, which have anti-inflammatory, anti-tumor, and anti-oxidative activities, but so far there has been no known A public report on the extraction of flavonoids from Sapona sinensis for the preparation of anti-sepsis acute kidney injury drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method and application of flavonoid compound saponin c in Chinese saponins
  • A kind of preparation method and application of flavonoid compound saponin c in Chinese saponins
  • A kind of preparation method and application of flavonoid compound saponin c in Chinese saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In specific implementation, the present invention specifically includes the following steps:

[0018] 1) prepare crude extract, the method is, 4.87kg of saponin thorns are pulverized, pass through 40 mesh sieves, each time with saponin thorn weight 8 times the volume concentration be 50% aqueous acetone solution (volume ratio), extract 3 times , 3 min each time, combine 3 extracts, concentrate under reduced pressure to a concentrate equivalent to 1.2 g / mL of crude drug, add 3 times the weight and volume of the concentrate to suspend in distilled water, and then add the same volume as the suspension in turn of petroleum ether and ethyl acetate, the solvent was recovered under reduced pressure to obtain the extract (89.5g crude extract of Saponin thorn) of the ethyl acetate extraction part;

[0019] 2) One-time separation, the method is that the extract of the ethyl acetate extraction site is mixed with 100 g of silica gel that has passed through a 100-200 mesh sieve, and...

Embodiment 2

[0023] In specific implementation, the present invention specifically includes the following steps:

[0024] 1) prepare crude extract, the method is, 5.04kg of saponin thorn is pulverized, pass through 40 mesh sieves, each time with saponin thorn weight 10 times the volume concentration is 50% aqueous acetone solution (volume ratio) extraction 3 times, each For 2min, combine the three extracts, concentrate under reduced pressure to a concentrate equivalent to 1.2g / mL of crude drug, add 4 times the weight and volume of the concentrate to suspend in distilled water, and then add the same volume of petroleum as the suspension in turn. Ether and ethyl acetate, and the solvent was recovered under reduced pressure to obtain the extract (85.6g crude extract of Saponin thorn) of the ethyl acetate extraction part;

[0025] 2) One-time separation, the method is that the extract of the ethyl acetate extraction site is mixed with 90 g of silica gel that has passed through a 100-200 mesh s...

Embodiment 3

[0029] In specific implementation, the present invention specifically includes the following steps:

[0030] 1) prepare crude extract, the method is, 5.62kg of saponin thorns are pulverized, pass through 40 mesh sieves, be 50% aqueous acetone solution (volume ratio) with the volume concentration of 9 times of saponin thorn weights each time, extract 3 times , 3 min each time, combine 3 extracts, concentrate under reduced pressure to a concentrate equivalent to 1.2 g / mL of crude drug, add 4 times the weight and volume of the concentrate to suspend in distilled water, and then add the same volume as the suspension in turn of petroleum ether and ethyl acetate, the solvent was reclaimed under reduced pressure to obtain the extract (92.6g of Saponin thorn crude extract) of the ethyl acetate extraction part;

[0031] 2) One-time separation, the method is that the extract of the ethyl acetate extraction site is mixed with 100 g of silica gel that has passed through a 100-200 mesh sie...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method and application thereof of the flavonoid compound saponin C from saponins, which can effectively solve the problem of rapidly extracting the flavonoid compound saponins C from saponins, and realize the preparation of anti-sepsis acute kidney injury drugs The technical solution is that the preparation method includes the following steps: 1) preparing the crude extract; 2) primary separation; 3) secondary separation; 4) purification; the preparation method of the present invention has high separation efficiency and short time , the solvent consumption is small, the sample recovery rate is high, and the purity of the target component obtained is high. The obtained saponin C has a good protective effect on the kidney injury induced by lipopolysaccharide, and is effectively used for the preparation of the treatment of sepsis or other infection factors. The drug for kidney damage is an innovation in the new use of the flavonoid compound saponin C in saponins.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of a flavonoid compound acacia new ketone C in Saponin thorn. Background technique [0002] Sepsis is a systemic inflammatory response to infection that often causes multiple tissue damage and dysfunction. Currently, sepsis has become the leading cause of death in intensive care unit (ICU) patients, the seventh leading cause of death in children aged 1-7 years and the eighth leading cause of death in people aged 65-75 years. Acute kidney injury (acute kidney injury) Kidney injury (AKI) is a common complication of sepsis and an independent risk factor for increased mortality in patients with sepsis, and its severity is significantly correlated with the high mortality of sepsis. With the aggravation of population aging in my country and the application of invasive medical methods and immunosuppressants, the incidence of acute kidney injury in sepsis is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/04A61P13/12
CPCC07D407/04A61P13/12
Inventor 李红伟曹彦刚郝志友李凯孟祥乐冯卫生
Owner HENAN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com