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Method for promoting inonotus obliquus to produce hypoglycemic active polysaccharides by adding pretreated birch sawdust

A technology of Inonotus obliquus and birch sawdust, which is applied in the field of hypoglycemic active polysaccharides, can solve the problems of low utilization rate of birch sawdust, and achieve the effect of increasing production and lowering blood sugar activity

Active Publication Date: 2021-06-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Birch is an important economic tree species in my country. However, the utilization rate of birch sawdust is low at present. Therefore, it is of great significance to develop a method of using birch sawdust as raw material and improving its utilization rate through biotechnology.

Method used

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  • Method for promoting inonotus obliquus to produce hypoglycemic active polysaccharides by adding pretreated birch sawdust
  • Method for promoting inonotus obliquus to produce hypoglycemic active polysaccharides by adding pretreated birch sawdust
  • Method for promoting inonotus obliquus to produce hypoglycemic active polysaccharides by adding pretreated birch sawdust

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Pulverize the birch, pass through a 40-mesh sieve, and take the part under the sieve to obtain the required birch chips; the ratio of birch chips and deionized water to liquid is 1:40 (g / ml), extracted with normal temperature water for 12 hours, and suction filtered The birch slag is removed to obtain the birch leaching juice, and the birch slag is set aside.

[0043] (2) Use the alkaline aqueous solution as an aqueous sodium hydroxide solution with a mass volume concentration of 2%, carry out alkali pretreatment with the birch chips and alkaline aqueous solution after the crushing and sieving according to the solid-to-liquid ratio of 1:15 (g / ml), and the alkali The pretreatment conditions are as follows: treat at 60°C for 2 hours, and the quality of birch wood chips is calculated by the absolute dry weight of wood chips.

[0044] (3) Use the birch wood chips obtained in the ozone treatment step (2), add the birch wood chips obtained by solid-liquid separation after...

Embodiment 2

[0048] (1) Pulverize the birch, pass through a 40-mesh sieve, and take the part under the sieve to obtain the required birch chips; the ratio of birch chips and deionized water to liquid is 1:40 (g / ml), extracted with normal temperature water for 12 hours, and suction filtered The birch slag is removed to obtain the birch leaching juice, and the birch slag is set aside.

[0049] (2) Alkali treatment is carried out with an aqueous sodium hydroxide solution with a mass volume concentration of 1%, the solid-to-liquid ratio of birch wood chips and alkaline aqueous solution is 1:15 (g / ml), and the conditions for alkali pretreatment are as follows: 70 ° C for 1 h , the quality of birch sawdust is calculated by absolute dry weight of sawdust.

[0050] (3) Use the birch chips obtained in the ozone treatment step (2), add the birch chips obtained by solid-liquid separation after the alkali treatment finishes in the sodium hydroxide aqueous solution of pH 9 (solid-liquid ratio is 1: 15 ...

Embodiment 3

[0054] (1) Pulverize the birch, pass through a 40-mesh sieve, take the lower part of the sieve, the ratio of birch chips and deionized water to liquid is 1:40 (g / ml), extract with normal temperature water for 12 hours, and remove the birch chips by suction filtration, that is Obtain birch leaching juice and birch slag for subsequent use.

[0055] (2) Alkali treatment is carried out with a sodium hydroxide aqueous solution with a mass volume concentration of 3%, the solid-to-liquid ratio of birch wood chips and alkaline aqueous solution is 1:15 (g / ml), and the conditions for alkali pretreatment are as follows: 50°C for 3h , the quality of birch sawdust is calculated by absolute dry weight of sawdust.

[0056] (3) Use the birch chips obtained in the ozone treatment step (2), add the birch chips obtained by solid-liquid separation after the alkali treatment finishes in the sodium hydroxide aqueous solution of pH 9 (solid-liquid ratio is 1: 15 (g / ml) ), the ozone ventilation rate...

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Abstract

The invention discloses a culture medium for promoting submerged fermentation of inonotus obliquus liquid to produce hypoglycemic active polysaccharides. The culture medium is composed of a liquid culture medium and birch sawdust pretreated by alkali and ozone; the liquid culture medium is composed of a solvent and a solute; the solvent is a birch leaching juice aqueous solution with the volume fraction of 10%-80%; the solute is glucose, tryptone, KH<2>PO<4> and MgSO<4>; and the pH value is natural. The culture medium is used for carrying out fermentation culture on inonotus obliquus. Results show that the yield of the inonotus obliquus mycelium polysaccharide obtained by the patent technology is remarkably increased and is increased by 52.92-79.51% compared with that of a basic culture medium added with washed birch sawdust; meanwhile, the inhibition rate of alpha-glucosidase is increased by 64.43-150.21%; the hypoglycemic activity of the inonotus obliquus polysaccharide is improved; and a new way is provided for comprehensive utilization of the birch sawdust.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, including Inonotus obliquus liquid submerged fermentation technology and birch sawdust combined pretreatment technology, in particular to a method of pretreating birch sawdust by combining birch extract juice and alkali combined with ozone, thereby promoting birch brown pores Bacteria liquid submerged fermentation decomposes and utilizes birch sawdust to produce hypoglycemic active polysaccharides. Background technique [0002] Inonotus obliquus belongs to Basidiomycotina, Phylomycetes, Polyporacea, Polyporaceae wood-rot fungi, and is also a precious white-rot fungus, which mainly grows and reproduces in birch, elm and other trees Skin underarms. Since the 16th and 17th centuries, in Ukraine, Belarus, Poland, and most of the Baltic countries, folk medicine has long used Inonotus obliquus to treat diabetes, intestinal cancer, and cardiovascular diseases. Inonotus obliquus polysa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
CPCC12P19/04
Inventor 刘萍王梦雅周霞赵喆禛刘晓李艺铭
Owner CHINA AGRI UNIV
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