Dunaliella salina capable of adsorbing heavy metals as well as application and preparation method of dunaliella salina and application of dunaliella salina in sea salt preparation
A technology for adsorbing heavy metals and salina, which is applied in the field of microbial engineering, can solve problems such as inability to adsorb heavy metals, and achieve good governance effects, low environmental pollution, and low energy consumption
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Embodiment 1
[0039] Example 1 Dunaliella salina with heavy metal adsorption
[0040] A type of salt algae that adsorbs heavy metals. It contains extracellular polysaccharides, proteins and fats. Among them, the mass of protein accounts for 24%-74% of the total mass of Dunaliella salina, and the mass of extracellular polysaccharides accounts for 2% of the total mass of Dunaliella salina. 16%; extracellular polysaccharides, proteins and fats form a network structure on the cell wall, the network structure has a certain negative charge, and has a large surface area and viscosity, which can enrich heavy metals through physical adsorption, and at the same time, Dunaliella cell wall The network structure on it has halogens, amino groups, amide groups, carbonyl groups, aldehyde groups, hydroxyl groups, mercaptans, thioethers, which can chemically react with heavy metal ions, thereby playing a role of chemical adsorption. At the same time, the cell membrane of the above-mentioned Dunaliella salina al...
Embodiment 2
[0041] Example 2 A method for purifying seawater by Dunaliella salina that adsorbs heavy metals
[0042] Utilizing the heavy metal-adsorbing Dunaliella salina provided in Example 1 can enrich the heavy metal ions in seawater through the above-mentioned dual adsorption of physical adsorption and chemical adsorption, thereby purifying the seawater. The specific method of purifying seawater is as follows:
[0043] c1) Sea water testing before purification
[0044] Take 20mL of Baume 3°C seawater from the tide pool, and use ICP-MS to detect the seawater before purification. The seawater before purification contains vanadium 11.625μg / L, chromium 0.483μg / L, cobalt 4.062μg / L, Nickel 2.011μgmL, copper 4.612μg / L, arsenic 19.136μg / L, strontium 887.08μg / L, molybdenum 0.546μg / L, silver 0.004μg / L, cadmium 0.003μg / L, gold 0.015μg / L, lead 0.03μg / L;
[0045] c2) Seawater detection after the adsorption and purification of Dunaliella salina
[0046] Take the Dunaliella salina in Example 1 and put it ...
Embodiment 3
[0048] Example 3 A preparation method of Dunaliella salina with heavy metal adsorption
[0049] A method for preparing the heavy metal-adsorbing Dunaliella salina described in embodiment 1, including the following steps in sequence:
[0050] a1) Take the Dunaliella wild strain as the starting algae strain, put the culture medium in the petri dish, put 3mL of the Dunaliella wild strain in the logarithmic growth phase into 3mL medium at 3000lx, temperature 25±1℃, light The dark cycle is 12h L / 12h D in a light incubator for light culture, and the culture cycle is 5 times; among them, the wild strain of Dunaliella is from the School of Environment and Life Sciences, Dalian University of Technology; the culture medium includes saturated brine and is in saturated brine Add 0.3g / L NaHCO 3 , 0.50g / L of NH 4 NO 3 , 0.08g / L of KH 2 PO 4 , 0.06mg / L of VB 1 , 2.0μg / L of VB 12 ;
[0051] a2) Choose three 30W UV lamps as the UV mutagenesis light source. The UV mutagenesis adopts dark operation, t...
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