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Method for catalytically synthesizing sucrose ester by using amorphous sucrase

An amorphous and sucrose ester technology, which is applied in the field of catalyzing and synthesizing sucrose esters by using amorphous sucrase, can solve the problems of increasing the difficulty of separation and purification of products, limited application of sucrose esters, etc., and achieves improved solubility, dissolution rate, and safety. The effect of improving and high enzymatic activity

Pending Publication Date: 2021-06-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a small portion of DMSO contained in the mixed system will increase the difficulty of product separation and purification, and limit the application of sucrose esters

Method used

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  • Method for catalytically synthesizing sucrose ester by using amorphous sucrase
  • Method for catalytically synthesizing sucrose ester by using amorphous sucrase
  • Method for catalytically synthesizing sucrose ester by using amorphous sucrase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First add 1.25g sodium caseinate to 500mL pure water, stir slowly at 50°C until completely dissolved, then add 123.75g sucrose, and dissolve completely to prepare a sucrose-sodium caseinate mixed solution. Add 0.3% Tween 20 and 0.3% methanol respectively to five parts of the above mixed solutions, mix well and carry out spray drying (conditions are: the water evaporation rate is 2kg / h, the inlet temperature is 170°C, and the outlet temperature is 70°C ), after spray-drying, dry in a vacuum oven at 50-60° C. for 5 hours to obtain amorphous sucrose with a yield of 96%.

Embodiment 2

[0032] In a 50mL round bottom flask, add 0.3g amorphous sucrose, 1.81mmol (470μL) vinyl laurate and 15mL tert-amyl alcohol, and add 1% sodium caseinate, 0.3% methanol and 0.2% Tween 20 at the same time, at 60 ℃ in a water bath and stir for 1h, then add 0.4g Molecular sieves were reacted in a water bath at 60°C for 12 hours. After the reaction was finished, it was detected by HPLC that no sucrose monoester was formed.

[0033] This result proves that other components in the above sucrose solution have no catalytic activity for sucrose ester synthesis.

Embodiment 3

[0035] Add Lipozyme TL IM, Novozym 435, Candida rugosa lipase, Pseudomonas fluorescens lipase, A. 15mL tert-amyl alcohol, 1.81mmol (470μL) vinyl laurate, 0.4g crystalline sucrose and 0.4g Molecular sieves were reacted in a water bath at 60°C for 12 hours. After the reaction, the concentrations of sucrose monoesters measured by HPLC were 8.2, 3.5, 2.9, 1.1, and 0.5 g / L, respectively. The results showed that the yield of sucrose monoesters was the highest when Lipozyme TL IM was used.

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Abstract

The invention discloses a method for catalytically synthesizing sucrose ester by using amorphous sucrase. According to the method, a proper amount of an organic solvent and a surfactant are added into a sucrose-sodium caseinate solution, and then the mixed solution is subjected to spray drying under proper conditions to obtain amorphous sucrose which is used as a substrate for catalytic synthesis of sucrose ester from lipase, so that the yield of sucrose ester can be greatly increased. According to the method, Lipozyme TL IM is adopted for catalytic synthesis of sucrose ester, the enzyme activity is high, the enzyme dosage is small, and the reaction condition is mild; besides, low-toxicity and low-boiling-point tert-amyl alcohol or tert-butyl alcohol is adopted as a reaction medium, so that the safety of the sucrose ester product is greatly improved, and the method can be applied to the fields of food and daily chemical products and is a green, efficient and low-cost sucrose ester production mode.

Description

[0001] (1) Technical field [0002] The invention relates to a method for catalyzing and synthesizing sucrose ester by using amorphous sucrase. [0003] (2) Background technology [0004] Sucrose fatty acid ester is a non-ionic surfactant with the advantages of non-toxicity, good biocompatibility, non-irritation, and biodegradability, so it is widely used in food, daily chemical, pharmaceutical and other industries. For example, sucrose monolaurate has a strong inhibitory effect on harmful bacteria such as Staphylococcus aureus, and can be used as a bacteriostatic agent in dairy products. [0005] In recent years, enzymatic synthesis of sucrose esters has attracted more and more attention. Most researchers use the mixed system of tert-amyl alcohol / DMSO (V:V=4:1) as the reaction medium to promote the dissolution of sucrose. The synthesis of sucrose laurate was catalyzed in DMSO mixed system, and the yield of sucrose monoester could reach 30g / L. However, due to the high toxici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12
CPCC12P19/12C12P7/6436
Inventor 陈小龙张珂睿詹蓉宋贝贝陆跃乐朱林江
Owner ZHEJIANG UNIV OF TECH
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