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Method for extracting asiaticoside-rich composition from centella asiatica

A technology of asiaticoside and a composition, applied in the field of natural substance extraction, can solve problems such as unfavorable industrialized production, complicated steps, small processing capacity, etc., and achieve the effects of being suitable for industrialized production, high industrial value, and improving extraction rate

Pending Publication Date: 2021-06-15
三益创价生物科技(深圳)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The separation methods of asiaticoside in triptoside (Centella asiatica) mainly include silica gel column chromatography, macroporous resin adsorption, two-phase extraction, ultrafiltration membrane separation, supercritical CO 2 Fluid extraction, high-speed countercurrent chromatography, etc., these methods have certain shortcomings and are not conducive to industrial production
The main problems are: complex process, excessive material consumption, low yield, and small processing capacity
[0005] For example, CN102532244A discloses a method for preparing high-purity asiaticoside, which uses solvent extraction to separate and obtain asiaticoside, which has complicated steps and high consumption, which is not conducive to industrial production
[0006] CN106946971A discloses a process for extracting Asiatic acid from Centella asiatica, through multiple decolorization and recrystallization methods, the process is complicated, the processing capacity is small, and the cost is high

Method used

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  • Method for extracting asiaticoside-rich composition from centella asiatica
  • Method for extracting asiaticoside-rich composition from centella asiatica
  • Method for extracting asiaticoside-rich composition from centella asiatica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Gotu Kola Water Extraction (CW), the preparation steps are as follows:

[0084] a. Wash and dry Gotu Kola first.

[0085] b. Weigh 1,000 grams of kerakoy root and put it into the pot.

[0086] c. Add 3000cc of water to the pot.

[0087] d. Boil the pot containing Gotu Kola and water, then boil on low heat for 1 hour.

[0088] e. Let the extract cool and filter to concentrate, leaving about 10-50cc.

[0089] f. Add 5-10 times of alcohol to the concentrated extract.

[0090] g. Put the alcoholic Gotu Kola extract into SFF fractionation.

[0091] Wherein, step e. The concentrated solution is further crystallized, and the product GCMS collection of spectra obtained is shown in figure 1 .

[0092] After step g.SFF fractionation, the product GCMS spectra of the first SFF fractionation tank, the second SFF fractionation tank and the bottom of the SFF extraction tank are shown in Figure 2 ~ Figure 4 .

Embodiment 2

[0094] Gotu Kola Extract (CE), the preparation steps are as follows:

[0095] a. Wash and dry Gotu Kola first.

[0096] b. Weigh 1,000 grams of gotu kola and put it into the ultrasonic extraction pot.

[0097] c. Add 3000cc of alcohol into the ultrasonic extraction pot, and conduct ultrasonic extraction for 1 hour.

[0098] d. Concentrate the extract by filtration, leaving about 500-1000cc.

[0099] e. Put the alcoholic Gotu Kola extract into SFF fractionation.

[0100] Wherein, step d. The concentrated solution is further crystallized, and the product GCMS collection of spectra obtained is shown in Figure 5 .

[0101] After step g.SFF fractionation, the GCMS spectrum of the product at the bottom of the first SFF fractionation tank and the SFF extraction tank is shown in Figure 6 ~ Figure 7 .

Embodiment 3

[0103] Gotu Kola Liquid Propane Extraction (CP), the preparation steps are as follows:

[0104] a. Firstly wash and dry the gotu kola and then pulverize it into a powder with a particle size of 0.5-5mm.

[0105] b. Weigh 350 grams of gotu root powder and put it into the extraction tank (the tank body is 2 liters).

[0106] c. The operating pressure is 0.40MPa, the temperature is 50°C, and alcohol with 5-10% propane volume is added.

[0107] d. The separation tank collects and extracts the extracted alcoholic Gotu Kola extract to obtain 80-200cc of Gotu Kola extract.

[0108] e. Extract 80-200cc of alcoholic Gotu Kola extract into SFF fractionation.

[0109] Wherein, step d. Gotu Kola extract is further crystallized, and the GCMS spectrum of the product obtained is shown in Figure 8 .

[0110] After step e.SFF fractionation, the product GCMS spectra of the first SFF fractionation tank, the second SFF fractionation tank and the bottom of the SFF extraction tank are shown in...

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Abstract

The invention discloses a method for extracting an asiaticoside-rich composition from centella asiatica, and relates to the technical field of natural product extraction. The method comprises the following steps: S1, washing and drying centella asiatica, and adding an extraction solvent for extraction to obtain centella asiatica extract; and S2, carrying out supercritical fractionation on the centella asiatica extract. According to the method for extracting the asiaticoside-rich composition from the centella asiatica, on the basis that the centella asiatica extract is obtained in an original extraction mode, the centella asiatica extract is further fractionated in a supercritical fractionation mode, the extraction rate is increased, and compared with traditional methods such as recrystallization, the supercritical fractionation mode is simpler in process, large in productivity, lower in cost, and higher in industrial value.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting a composition rich in asiaticoside from triptophylla. Background technique [0002] Gotu Kola, aliased as Centella Asiatica, Horseshoe Grass, Bengdawan, Cockleshell Grass, Dengzhancao, Old Ear, Coppermoss, Umbelliferae herb. The chemical components contained in Gotu Kola mainly include asiaticoside, madecassoside, ginsengniside, asiatic acid, asiatica sugar, kaempferol, quercetin, etc. In recent years, studies have found that Gotu Kola (Centella asiatica) can tighten the connection between the epidermis and the dermis, making the skin soft, helping to solve the phenomenon of skin relaxation (especially for postpartum mothers), making the skin smooth and elastic; helping to promote the growth of the dermis Collagen is formed to regenerate fibrin and reconnect to make the skin firm and smooth. It also helps damaged tissue heal and firm t...

Claims

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Application Information

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IPC IPC(8): B01D11/02B01D3/14
CPCB01D11/028B01D3/143B01D11/0288
Inventor 吴宇建叶胜仁林圣富蔡庆瑞席美华
Owner 三益创价生物科技(深圳)有限公司