ACE inhibitory peptide as well as preparation method and application thereof

A technology for inhibiting peptides and reactions, applied in the field of ACE inhibitory peptides and their preparation, can solve the problem of high energy consumption, and achieve the effects of simple preparation process, small molecular weight, and strong ACE inhibitory activity

Pending Publication Date: 2021-06-15
河南省农业科学院农副产品加工研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The temperature used in traditional subcritical water technology is above 200 °C, which requires high energy consumption

Method used

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  • ACE inhibitory peptide as well as preparation method and application thereof
  • ACE inhibitory peptide as well as preparation method and application thereof
  • ACE inhibitory peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for preparing ACE-inhibiting peptide LFRAF from high-temperature sesame cake, which specifically includes the following steps:

[0045] 1) Sesame raw material protein extraction:

[0046]The high-temperature sesame cake was pulverized and degreased using a Soxhlet extractor. The solvent was petroleum ether, the extraction temperature was 80°C, and the degreasing was performed for 6 hours. The degreased high-temperature sesame cake and distilled water were mixed evenly at a ratio of 1 g: 20 mL, then the pH was adjusted to 11.0 with 6 mol / L NaOH, stirred at room temperature for 60 min, and centrifuged at 5000 r / min for 20 min at 4 °C. Min, after collecting the supernatant, adjust the pH to 4.3 with 6 mol / L HCl, let it stand for 20 minutes, and then centrifuge to collect the protein precipitate, freeze-dry and store at 4°C to obtain the raw material protein of sesame;

[0047] 2) Low temperature subcritical water hydrolysis:

[0048] Mix the sesame raw material ...

Embodiment 2

[0052] A method for artificially synthesizing ACE inhibitory peptides, which is artificially synthesized by a conventional solid-phase synthesis method in the art. The basic process is as follows: first, connect an amino acid whose amino group is protected by Fmoc group to the insoluble solid phase carrier Wang resin, and then remove the protecting group of the amino group, and the first amino acid is connected to the solid phase carrier; The carboxyl group of the second amino acid protected by the Fmoc group is activated with a condensing agent, and the activated amino acid reacts with the amino group of the first amino acid that has been attached to the solid phase carrier to form a peptide bond. A dipeptide with a protecting group. Repeat the above-mentioned peptide bond formation reaction to grow the peptide chain from the C-terminus to the N-terminus until the required length of the peptide chain is reached, and finally cut to obtain the target polypeptide. Pentapeptides...

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Abstract

The invention provides a class of ACE inhibitory peptides. The amino acid sequences of the ACE inhibitory peptides are respectively as follows: Leu-Phe-Arg-Ala-Phe (LFRAF), Leu-Phe-Arg-Ala-Arg (LFRAR), Leu-Phe-Arg-Ala-Val (LFRAV), Leu-Phe-Arg-Tyr-Phe (LFRYF), Leu-Phe-Arg-Asn-Phe (LFRNF), Leu-Phe-Arg-Glu-Phe (LFREF), Ala-Phe-Arg-Ala-Phe (AFRAF), Met-Phe-Arg-Ala-Phe (MFRAF) and Val-Phe-Arg-Ala-Phe (VFRAF). The ACE inhibitory peptide has the advantages of strong ACE inhibitory activity, small molecular weight, low preparation cost and simple process, is easy for industrial production, can prevent, relieve and assist treatment of hypertension after being taken for a long time, and has wide application and development prospects in the fields of foods, medicines, health care products and the like.

Description

technical field [0001] The invention belongs to the technical field of biological small molecule active peptides, and in particular relates to a class of ACE inhibitory peptides and a preparation method and application thereof. Background technique [0002] Hypertension is a disease that is extremely harmful to human health and is the main pathogenic factor of coronary heart disease, stroke, and heart failure. Angiotensin-converting enzyme (ACE) plays an important role in regulating blood pressure. Clinically, most hypertensive patients use synthetic ACE inhibitors (such as captopril) to control the increase in blood pressure. Although these drugs have significant curative effects, they often cause side effects such as taste disturbance and excessively low blood pressure. The ACE inhibitory peptide isolated from natural food has the advantages of no effect on normal blood pressure and no toxic side effects, although the antihypertensive effect is not as obvious as that of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/04C07K1/06C07K1/34C07K1/16C07K1/14A61K38/08A61P9/12
CPCA61K38/00A61P9/12C07K7/06
Inventor 王瑞丹芦鑫高锦鸿贾聪孙强黄纪念
Owner 河南省农业科学院农副产品加工研究中心
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