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Method for preparing functional coating containing nano-silver antibacterial peptide on metal surface

A metal surface, antibacterial peptide technology, applied in coating, medical science, prosthesis, etc., can solve the problem of inability to cope with bacterial infection environment, drug resistance, etc., to solve bacterial drug resistance, reduce dosage, and increase service life Effect

Active Publication Date: 2021-06-18
NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Currently, common antibiotic-loaded coatings are likely to cause bacterial resistance, and cannot cope with bacterial infection environments with higher bacterial concentrations or stronger bacterial activity, and high Concentration of antibacterial agents will produce potential biological toxicity, how to achieve long-term and high-efficiency antibacterial effect at low concentration and dosage has become a challenge

Method used

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  • Method for preparing functional coating containing nano-silver antibacterial peptide on metal surface
  • Method for preparing functional coating containing nano-silver antibacterial peptide on metal surface

Examples

Experimental program
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Effect test

Embodiment 1

[0030] This embodiment includes the following steps:

[0031] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0032] The silk fibroin stock solution is diluted with deionized water into a silk fibroin solution with a mass concentration of 2%, and then 17mg of silver nitrate powder and 4mg of antimicrobial peptide powder are added to 10mL of the silk fibroin solution with a mass concentration of 2% and mixed to obtain a silk fibroin solution containing 2% mass concentration. The mixed solution A of protein, silver ion and antimicrobial peptide and pH is 8.5;

[0033] Step 2. Place the mixed solution A obtained in step 1 under a 40W ultraviolet lamp for irradiation and reduction for 0.5h, so that the color of the mixed solution A gradually changes from milky white to dark brown, and obtains silk fibroi...

Embodiment 2

[0054] This embodiment includes the following steps:

[0055] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0056] The silk fibroin stock solution was diluted with deionized water into a silk fibroin solution with a mass concentration of 3%, and then 10 mg of silver nitrate powder and 8 mg of antimicrobial peptide powder were added to 10 mL of a silk fibroin solution with a mass concentration of 3% and mixed to obtain a silk fibroin solution containing 3% mass concentration. Protein, silver ion and antimicrobial peptide and the mixed solution A that pH is 8;

[0057] Step 2. Place the mixed solution A obtained in step 1 under a 40W ultraviolet lamp for irradiation and reduction for 1 hour, so that the color of the mixed solution A gradually changes from milky white to dark brown, and a mixed soluti...

Embodiment 3

[0061] This embodiment includes the following steps:

[0062] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0063] Dilute the silk fibroin stock solution with deionized water into a silk fibroin solution with a mass concentration of 5%, then add 200 mg of silver nitrate powder and 10 mg of antimicrobial peptide powder into 10 mL of a silk fibroin solution with a mass concentration of 5% and mix to obtain a silk fibroin solution containing 5% mass concentration. Protein, silver ion and antimicrobial peptide and the mixed solution A that pH is 10;

[0064] Step 2. Place the mixed solution A obtained in step 1 under a 40W ultraviolet lamp for irradiation and reduction for 2 hours, so that the color of the mixed solution A gradually changes from milky white to dark brown, and a mixed solution containing ...

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Abstract

The invention discloses a method for preparing a functional coating containing nano-silver antibacterial peptide on a metal surface. The method comprises the steps that step one, silver nitrate powder and antibacterial peptide powder are added into a silk fibroin solution to be mixed, and a mixed solution A is obtained; step two, the mixed solution A is subjected to ultraviolet radiation reduction to obtain a mixed solution B; step three, after standardization of a Tris solution, dopamine hydrochloride is added, the metal is soaked, and the metal with a polydopamine auxiliary layer on the surface is obtained; and step four, the metal with the polydopamine auxiliary layer on the surface is soaked in the mixed solution B, and the functional coating containing the nano-silver antibacterial peptide is formed on the surface of the metal. According to the invention, silver ions are reduced into nano-silver through silk fibroin by adopting an ultraviolet radiation method, the particle size and morphology of nano-silver particles are effectively controlled, coordination bonds are formed between antibacterial peptides and nano-silver, enrichment of the antibacterial peptides on the surfaces of the nano-silver particles is promoted, the antibacterial peptides are in a nano-scale dispersed state in the nano-silver-containing antibacterial peptide functional coating, and long-term high-efficiency sterilization under the condition of low antibacterial agent dosage is realized.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a method for preparing a functional coating containing nano-silver antimicrobial peptide on a metal surface. Background technique [0002] Medical pure titanium has been widely used in the field of clinical medicine because of its excellent biocompatibility. As early as the 1950s, pure titanium was processed into bone plates, bone nails, femoral heads and other implanted medical devices for orthopedic repair operations. However, as a common postoperative complication, bacterial infection caused by medical devices has become one of the important problems to be solved in the field of medicine in the 21st century. According to reports, the annual incidence of orthopedic implant-related infections in the United States reaches about 4.3%. According to the relevant data in the "Practical Handbook of Nosocomial Infection Prevention and Control" promulgated by the World ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/54A61L27/30A61L27/22A61L27/06
CPCA61L27/34A61L27/54A61L27/06A61L27/227A61L27/306A61L2300/104A61L2420/06A61L2420/08A61L2300/252A61L2300/608A61L2300/404A61L2300/41A61L2300/45C08L89/00C08L79/04
Inventor 周文昊余森荆磊张亚峰汶斌斌
Owner NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
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