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Method for separating and purifying dilinoleoylphosphatidylcholine and dilinoleoylphosphatidylcholine product

A technology for purifying dilinoleoylphosphatidylcholine and dilinoleoylphosphatidylcholine, which is applied in chemical instruments and methods, edible phospholipid compositions, organic chemistry, etc., can solve adverse product safety and environmental friendliness, purity Limited and other problems, to achieve the effect of strong separation ability, simple and controllable operation, and good repeatability

Active Publication Date: 2021-06-18
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the DLPC product prepared by normal phase column has limited purity on the one hand, and on the other hand, most of the solvents used are dichloromethane, n-hexane, etc., which is not conducive to product safety and environmental friendliness.

Method used

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  • Method for separating and purifying dilinoleoylphosphatidylcholine and dilinoleoylphosphatidylcholine product
  • Method for separating and purifying dilinoleoylphosphatidylcholine and dilinoleoylphosphatidylcholine product

Examples

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Embodiment 1

[0031] This embodiment provides a method for separating and purifying dilinoleoylphosphatidylcholine and dilinoleoylphosphatidylcholine products, the separation and purification flow chart is as follows figure 1 shown.

[0032] Preparation of sample solution: take 2 g of high-purity phosphatidylcholine (the content of phosphatidylcholine is 90 wt%, and the content of dilinoleoylphosphatidylcholine is 28 wt%), and dissolve the sample in 0.5 mL ethanol.

[0033] Separation and purification: the sample solution is separated and purified by a preparative column system, all the sample solution is injected, and the preparative chromatographic column uses C18 packing with a particle size of 20-35 μm and a pore size of The packing mass is 45g, the flow rate is 0.1bv / min; the elution solvent is methanol solution, and the detection wavelength is 203nm. Receive the eluent from the peak of the target peak until the target peak returns to the baseline position, receive the eluent every 0...

Embodiment 2

[0035] Sample loading solution preparation: take 0.4 g of high-purity phosphatidylcholine (the content of phosphatidylcholine is 93wt%, the content of dilinoleoylphosphatidylcholine is 32wt%), and dissolve the sample in 0.5mL ethanol.

[0036] Separation and purification: the sample solution is separated and purified by a preparative column system, all the sample solution is injected, and the preparative chromatographic column uses C18 packing with a particle size of 20-35 μm and a pore size of The packing mass is 40g, the flow rate is 0.3bv / min; the elution solvent is aqueous methanol (65:35, v / v), and the detection wavelength is 203nm. Receive the eluent from the peak of the target peak until the target peak returns to the baseline position, receive the eluent every 0.5min, analyze the purity of DLPC by HPLC-UV, combine the eluents that detect DLPC, and combine the elution The solution was subjected to low-temperature precipitation and drying, and the purity and recovery ra...

Embodiment 3

[0038] Preparation of sample solution: take 4 g of high-purity phosphatidylcholine (the content of phosphatidylcholine is 95wt%, and the content of dilinoleoylphosphatidylcholine is 36wt%), and dissolve the sample in 0.5mL ethanol.

[0039] Separation and purification: the sample solution is separated and purified by a preparative column system, all the sample solution is injected, and the preparative chromatographic column uses C18 packing with a particle size of 20-35 μm and a pore size of The packing mass is 35g, the flow rate is 0.6bv / min; the elution solvent is aqueous methanol (50:50, v / v), and the detection wavelength is 203nm. Receive the eluent from the peak of the target peak until the target peak returns to the baseline position, receive the eluent every 0.5min, and combine the eluents that detect DLPC through HPLC-UV analysis, and then carry out the combined eluent. Low-temperature precipitation and drying, the purity and recovery rate of the obtained dilinoleoylp...

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Abstract

The invention belongs to the field of phospholipid processing, and particularly relates to a method for separating and purifying dilinoleoylphosphatidylcholine and a dilinoleoylphosphatidylcholine product. The method comprises the following steps: (1) preparation of a sample loading solution: dissolving high-purity phosphatidylcholine into low-carbon alcohol to obtain a sample solution; and (2) separation and purification: carrying out separation and purification on the sample solution by adopting a reversed-phase column system, then carrying out elution, collecting an eluent, and then carrying out low-fat desolvation on the eluent to obtain the dilinoleoylphosphatidylcholine product. According to the invention, the DLPC content is greatly increased, the purity is increased from 30% to 90% or above, and the recovery rate is higher than 70%; the low-carbon alcohol or the low-carbon alcohol water solution is used as the eluent, so that recycling is facilitated, and the process is green and environment-friendly; and the preparative chromatographic column is adopted for separation and purification, so that the separation capacity is high, repeatability is good, operation is easy and controllable, and industrial production is easy to achieve.

Description

technical field [0001] The invention belongs to the field of phospholipid processing, and more specifically relates to a method for separating and purifying dilinoleoylphosphatidylcholine and a product of dilinoleoylphosphatidylcholine. Background technique [0002] Dilinoleoylphosphatidylcholine (DLPC) is the main active component of lecithin (PC) in soybean, and it is the main molecular species in polyene phospholipid (PPC), which has very important biological functions. Polyene phosphatidylcholine contains about 40-52% as dilinoleoylphosphatidylcholine (DLPC). Jaime Ponia Chik et al. proposed that DLPC may be the reason for preventing fibrosis and inhibiting the activation of hepatic stellate cells, and DLPC has Antioxidant effect, reduces cellular damage induced by lipid peroxidation in vitro by reducing the formation of lipid peroxidation products. Studies by Khursheed P. Navder and others have proved that DLPC has an antioxidant effect and can significantly protect hu...

Claims

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Application Information

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IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 张维农刘思敏从艳霞齐玉堂
Owner WUHAN POLYTECHNIC UNIVERSITY
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