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Complex microbial inoculant for promoting germination and growth of dendrobium officinale seeds

A technology of compound bacterial agent and Dendrobium candidum, applied in the direction of plant growth regulator, plant growth regulator, biocide, etc., can solve the problems of thick roots of asexual seedlings, low survival rate of transplanting, slow growth, etc.

Pending Publication Date: 2021-06-18
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the Dendrobium tissue culture system has been successfully established, which can reproduce Dendrobium asexual seedlings in large quantities, but the roots of the asexual seedlings are thick and the root hairs are underdeveloped, which cannot meet the needs of plants for water and nutrients, so the transplanting survival rate is low, and the growth cycle is slow. In addition, the medicinal value of the asexual seedlings cultivated by tissue culture is very different from that of the wild species

Method used

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  • Complex microbial inoculant for promoting germination and growth of dendrobium officinale seeds
  • Complex microbial inoculant for promoting germination and growth of dendrobium officinale seeds
  • Complex microbial inoculant for promoting germination and growth of dendrobium officinale seeds

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Embodiment 1

[0015] Embodiment 1, the composite bacterial agent of Dendrobium candidum, comprises the fungi of the genus Tulasnella sp., the fungus of the genus Fusarium (Fusarium sp.), the fungus of the genus Mycena (Mycena sp.) and the fungus of the genus Armillaria sp. .) Fungi. All of the above strains can be obtained in a commercially available form, for example, they can be purchased from Shandong Tuopu Bioengineering Co., Ltd.: M4103 fungi of the genus Tulasnella sp., fungi of the genus Fusarium sp. M4112, Fungi of the genus Mycena sp. for M4160, fungi of the genus Armillaria sp. for M4173.

[0016] The above-mentioned fungi of the genus Tulasnella sp., fungi of the genus Fusarium, fungi of the genus Mycena sp. and fungi of the genus Armillaria sp. were made into concentrations of 30 CFU / mL suspension diluent. Then mix according to the volume ratio of 1:1:1:1 to form a composite bacterial solution, and finally add 5% PTA silkworm-P4 and mix. That is, 5g of PTA silkworm-P4 was ad...

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Abstract

The invention discloses a complex microbial inoculant for promoting germination and growth of dendrobium officinale seeds. The complex microbial inoculant comprises a complex bacterial fluid composed of Tulasnella sp. fungus, Fusarium sp. fungus, Mycena sp. fungus and Armillaria sp. fungus; and the composite bacterial agent is obtained by adding 5% PTA silkwor-P4 into the complex bacterial liquid. By adopting the complex microbial inoculant disclosed by the invention, the germination of dendrobium officinale seeds can be promoted, and dendrobium officinale seedlings with mycorrhiza are formed, so that the growth of dendrobium officinale is promoted.

Description

technical field [0001] The invention relates to the field of biological bacterial fertilizer, in particular to a compound bacterial agent for promoting the germination and growth of dendrobium officinale seeds. Background technique [0002] Dendrobium officinale is a perennial epiphyte of the genus Dendrobium orchidaceae, which is widely used in research and development in the field of medicine. Dendrobium officinale is a traditional precious Chinese medicine in my country, and it is the first of the "nine immortal grasses" in China. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. Modern medical research shows that Dendrobium candidum can effectively enhance the body's immunity, and has obvious effects in anti-cancer, anti-oxidation, anti-aging, anti-fatigue, promoting digestion, lowering blood sugar and promoting bone cell proliferation. At present, due to the slow growth of Dendrobium candidum, strict requireme...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01N47/44A01P1/00A01P21/00C12R1/645C12R1/77
CPCC12N1/14A01N63/30A01N47/44
Inventor 徐涛徐雯欣黄路瑶范浩宇高海立
Owner ZHEJIANG SCI-TECH UNIV