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Silk protein nanofiber gel dressing with pressure spreadability and preparation method and application thereof

A technology of nanofiber and silk protein, which is applied in pharmaceutical formulations, bandages, drug delivery, etc., to achieve the effect of simple preparation method, stable performance, and improved vascularization level

Active Publication Date: 2021-06-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, considering the significant advantages of silk protein in different tissue repair, gel preparation, and drug loading and controlled release, it is of great significance to develop and prepare a gel dressing that can meet the special requirements of skin flap vascularization using silk protein nanofibers as a matrix. Certainly feasible, but similar to other biomaterials, silk protein nanofiber gels with the above pressure spreadability have not been reported

Method used

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  • Silk protein nanofiber gel dressing with pressure spreadability and preparation method and application thereof
  • Silk protein nanofiber gel dressing with pressure spreadability and preparation method and application thereof
  • Silk protein nanofiber gel dressing with pressure spreadability and preparation method and application thereof

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preparation example Construction

[0034] The invention provides a method for preparing a pressure-spreadable silk protein nanofiber gel dressing, comprising the following steps: S1) mixing and stirring the first silk protein nanofiber gel with water to obtain silk protein with changed rheology nanofiber gel; mixing the drug for promoting angiogenesis with the second silk protein nanofiber gel to obtain the drug-loaded silk protein nanofiber gel; S2) combining the rheologically changed silk protein nanofiber gel with the drug-loaded The silk protein nanofiber gel is mixed and stirred to obtain a silk protein nanofiber gel dressing with pressure spreadability.

[0035]The present invention first regulates the rheology of the silk protein nanofiber gel to an appropriate range by adjusting the concentration of silk protein nanofibers, and then further regulates the rheology through appropriate shearing, and finally obtains ideal rheological properties and realizes coagulation. Gentle pressure of the glue spreads. ...

Embodiment 1

[0050](1) Add deionized water to the silk protein nanofiber gel (the crystallinity of silk protein nanofiber is 40%, the diameter is 20nm, and the length is 1μm) that concentration is 2.5wt%, and the concentration is adjusted to 0.8wt%. The magnetic stirrer stirred for 24 hours at a rate of 800 rpm;

[0051] (2) The deferoxamine powder is mixed with the silk protein nanofiber gel (the crystallinity of the silk protein nanofiber is 40%, the diameter is 20nm, and the length is 1 μm) with a concentration of 1.2wt%, so that the deferoxamine concentration is 1mg / ml, stirred at a speed of 200rpm for 4 hours, then centrifuged at a speed of 12000rpm for 30 minutes, and the bottom was a drug-loaded silk protein nanofiber gel;

[0052] (3) Mix the above-mentioned drug-loaded nanofiber gel with the gel stirred in (1) according to the volume ratio of 1:15, and then stir at a speed of 1000rpm for 6 hours, so that the two gels are fully mixed, and mixed Adjust the rheology of the gel to a...

Embodiment 2

[0059] (1) Add deionized water to the silk protein nanofiber gel (the crystallinity of the silk protein nanofiber is 40%, the diameter is 20nm, and the length is 1 μm) with a concentration of 2wt%, and the concentration is adjusted to 1wt%, using magnetic stirring Under the speed of 900rpm, stirrer 24 hours;

[0060] (2) deferoxamine powder is mixed with the silk protein nanofiber gel (the crystallinity of silk protein nanofiber is 40%, diameter is 20nm, length is 1 μ m) that concentration is 1wt%, makes deferoxamine concentration be 2mg / ml, stirred at a speed of 200rpm for 6 hours, then centrifuged at a speed of 14000rpm for 25 minutes, and the bottom was a drug-loaded silk protein nanofiber gel;

[0061] (3) Mix the above-mentioned drug-loaded nanofiber gel with the gel stirred in (1) according to the volume ratio of 1:24, and then stir at a speed of 600rpm for 14 hours, so that the two gels are fully mixed, and mixed Adjust the rheology of the gel to an appropriate level;...

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Abstract

The invention provides a preparation method of a silk protein nanofiber gel dressing with pressure spreadability, the preparation method comprises the following steps: S1) mixing and stirring first silk protein nanofiber gel and water to obtain silk protein nanofiber gel with changed rheological property; mixing a drug for promoting vascular regeneration with the second fibroin nanofiber gel to obtain drug-loaded fibroin nanofiber gel; and S2) mixing and stirring the silk protein nanofiber gel with the changed rheological property and the drug-loaded silk protein nanofiber gel to obtain the silk protein nanofiber gel dressing with the pressure spreadability. Compared with the prior art, by regulating and controlling the rheological property of the silk protein nanofiber gel, the special function that the gel is spread and shaped under mild pressure to form an ultra-thin coating is achieved, meanwhile, silk protein nanofiber serves as a carrier to load a medicine for promoting revascularization, and therefore the special requirement for uniform vascularization of skin flaps is met.

Description

technical field [0001] The invention belongs to the technical field of wound repair, and in particular relates to a pressure-spreadable silk protein nanofiber gel dressing, a preparation method and application thereof. Background technique [0002] Skin flap transplantation is a common treatment method for plastic surgery, burns, trauma, etc. It can provide effective protection for wounds and restore skin function. The key to successful flap transplantation lies in the rapid remodeling of the flap and the vascularized network of the wound base, so as to provide nutrition for the flap and avoid flap necrosis. Flap necrosis due to insufficient blood supply is an important factor in the failure of flap transplantation, and it is also a core problem that restricts the size, shape and application of flaps. Therefore, how to improve the ability of flaps to promote vascularization and promote the rapid and effective reconstruction of flaps and wound-based vascularization networks ...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0047A61L26/008A61L26/0066A61L26/0061A61L2400/12A61L2300/602A61L2300/412A61L2300/252C08L89/00
Inventor 伍丽君吕强高素玥丁召召
Owner SUZHOU UNIV
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