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Silk protein nanofiber gel dressing with pressure spreadability, its preparation method and application

A technology of nanofibers and silk proteins, which is applied in pharmaceutical formulations, bandages, drug delivery, etc., to achieve the effects of simple preparation method, promoting vascularization ability, and stable performance

Active Publication Date: 2022-06-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, considering the significant advantages of silk protein in different tissue repair, gel preparation, and drug loading and controlled release, it is of great significance to develop and prepare a gel dressing that can meet the special requirements of skin flap vascularization using silk protein nanofibers as a matrix. Certainly feasible, but similar to other biomaterials, silk protein nanofiber gels with the above pressure spreadability have not been reported

Method used

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  • Silk protein nanofiber gel dressing with pressure spreadability, its preparation method and application
  • Silk protein nanofiber gel dressing with pressure spreadability, its preparation method and application
  • Silk protein nanofiber gel dressing with pressure spreadability, its preparation method and application

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preparation example Construction

[0034] The present invention provides a method for preparing a silk protein nanofiber gel dressing with pressure spreadability, comprising the following steps: S1) mixing and stirring the first silk protein nanofiber gel with water to obtain a silk protein with changed rheology Nanofiber gel; mixing the drug for promoting angiogenesis with the second silk protein nanofiber gel to obtain drug-loaded silk protein nanofiber gel; S2) mixing the rheologically modified silk protein nanofiber gel with drug loading The silk protein nanofiber gel is mixed and stirred to obtain a silk protein nanofiber gel dressing with pressure spreadability.

[0035]The present invention firstly regulates the rheology of the silk protein nanofiber gel to a suitable range by adjusting the concentration of the silk protein nanofibers, and then further regulates the rheology through suitable shearing action, and finally obtains ideal rheological properties and realizes coagulation. Mild pressure of the g...

Embodiment 1

[0050](1) Add deionized water to the silk protein nanofiber gel with a concentration of 2.5wt% (the crystallinity of silk protein nanofibers is 40%, the diameter is 20 nm, and the length is 1 μm), and the concentration is adjusted to 0.8wt%, using The magnetic stirrer was stirred at 800 rpm for 24 hours;

[0051] (2) Mix the deferoxamine powder with the silk protein nanofiber gel with a concentration of 1.2 wt% (the crystallinity of the silk protein nanofibers is 40%, the diameter is 20 nm, and the length is 1 μm), so that the concentration of deferoxamine is 1 mg / ml, stirred at 200 rpm for 4 hours, then centrifuged at 12,000 rpm for 30 minutes, and the bottom was a drug-loaded silk protein nanofiber gel;

[0052] (3) Mix the above-mentioned drug-loaded nanofiber gel and the gel with the stirring treatment in (1) according to the volume ratio of 1:15, and then stir for 6 hours at a rotating speed of 1000 rpm, so that the two gels are fully mixed, and mixed The rheology of th...

Embodiment 2

[0059] (1) Add deionized water to the silk protein nanofiber gel with a concentration of 2 wt% (the crystallinity of silk protein nanofibers is 40%, the diameter is 20 nm, and the length is 1 μm), the concentration is adjusted to 1 wt%, and magnetic stirring is used. The machine was stirred at a speed of 900 rpm for 24 hours;

[0060] (2) Mix the deferoxamine powder with the silk protein nanofiber gel with a concentration of 1 wt% (the crystallinity of the silk protein nanofibers is 40%, the diameter is 20 nm, and the length is 1 μm), so that the deferoxamine concentration is 2 mg / ml, stirred at 200 rpm for 6 hours, then centrifuged at 14,000 rpm for 25 minutes, and the bottom was a drug-loaded silk protein nanofiber gel;

[0061] (3) Mix the above-mentioned drug-loaded nanofiber gel and the gel with stirring in (1) according to the volume ratio of 1:24, and then stir for 14 hours at a rotating speed of 600 rpm, so that the two gels are fully mixed, and mixed The rheology of...

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Abstract

The invention provides a method for preparing a pressure-spreadable silk protein nanofiber gel dressing, comprising the following steps: S1) mixing and stirring the first silk protein nanofiber gel with water to obtain silk protein with changed rheology nanofiber gel; mixing the drug for promoting angiogenesis with the second silk protein nanofiber gel to obtain the drug-loaded silk protein nanofiber gel; S2) combining the rheologically changed silk protein nanofiber gel with the drug-loaded The silk protein nanofiber gel is mixed and stirred to obtain a silk protein nanofiber gel dressing with pressure spreadability. Compared with the prior art, the present invention realizes the special function of spreading and shaping the gel under mild pressure to form an ultra-thin coating by regulating the rheological properties of the silk protein nanofiber gel, and at the same time uses the silk protein nanofiber as a carrier to load and promote blood vessels Regenerative medicine, thus meeting the specific needs of homogeneous vascularization of skin flaps.

Description

technical field [0001] The invention belongs to the technical field of wound repair, and in particular relates to a silk protein nanofiber gel dressing with pressure spreadability, a preparation method and application thereof. Background technique [0002] Flap transplantation is a commonly used treatment method for plastic surgery, burns, trauma, etc. It can provide effective protection for wounds and restore skin function. The key to successful flap transplantation is the rapid remodeling of the flap and the vascularized network of the wound base, which can provide nutrition to the flap and avoid flap necrosis. Flap necrosis due to insufficient blood supply is an important factor in the failure of flap transplantation, and it is also the core problem that restricts the size, shape and application of flaps. Therefore, how to improve the vascularization-promoting ability of the flap and promote the rapid and effective reconstruction of the vascularization network of the fla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0047A61L26/008A61L26/0066A61L26/0061A61L2400/12A61L2300/602A61L2300/412A61L2300/252C08L89/00
Inventor 伍丽君吕强高素玥丁召召
Owner SUZHOU UNIV
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