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Electrochemical sensor capable of simultaneously detecting glucose and uric acid

A sensor and electrochemical technology, applied in the field of non-enzyme electrochemical sensors, can solve the problems of simultaneous detection of glucose and other problems, achieve excellent catalytic ability, reduce costs, and overcome the effects of easy deactivation

Active Publication Date: 2021-06-22
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that this type of non-enzymatic uric acid electrochemical sensor can achieve simultaneous detection with ascorbic acid, dopamine, epinephrine, nitrite ions, etc., but cannot achieve simultaneous detection with glucose

Method used

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  • Electrochemical sensor capable of simultaneously detecting glucose and uric acid
  • Electrochemical sensor capable of simultaneously detecting glucose and uric acid
  • Electrochemical sensor capable of simultaneously detecting glucose and uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Ultrasonic the ITO conductive glass with a cleaning agent for 30 minutes, then clean it with distilled water, then use acetone, ethanol, and distilled water to sonicate for 5 minutes, then soak the ITO conductive glass in sodium hydroxide solution for 8 hours, and wash it with deionized water Blow dry after rinsing;

[0054] (2) 2.4×10 -2 mol / L sodium citrate solution and 2.4×10 -2 Take 0.5 mL of each mol / L tetrachloroauric acid solution, set the volume to 50 mL, slowly add 0.1 mol / L sodium borohydride solution dropwise under magnetic stirring, stop the dropwise addition when the solution changes from colorless to pink, and prepare A gold nanoparticle solution with an average particle diameter of 158nm was obtained;

[0055] (3) get the gold nanoparticle solution in step (2), 30% stock gel solution, 1.0mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH=8.8), 5% sodium lauryl sulfate solution, 5% ammonium persulfate solution and tetramethylethylenediamine so...

Embodiment 2

[0062] The preparation method of the sensor based on gold nanoparticle gradient polyacrylamide gel modification described in this embodiment is different from Example 1 in that: in step (3), gold nanoparticle solution, 30% stock gel solution , 2.0mol / L of 3-hydroxyaminomethane hydrochloric acid buffer (pH=8.8), 15% sodium lauryl sulfate solution, 15% ammonium persulfate solution and tetramethylethylenediamine solution according to volume ratio 4.6: 2.7 : 2.5: 0.1: 0.1: 0.006 mixed to prepare gel solution I; in step (5), gold nanoparticle solution, 30% stock gel solution, 1.5mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH =6.8), 15% sodium lauryl sulfate solution, 15% ammonium persulfate solution and tetramethylethylenediamine solution are mixed according to volume ratio 4.1: 1: 0.75: 0.06: 0.06: 0.006 to obtain a gel solution Ⅱ; The sensor based on gradient polyacrylamide gel modification of gold nanoparticles was prepared.

[0063] The 30% reserve gel solution was p...

Embodiment 3

[0065] The preparation method of the sensor based on gold nanoparticle gradient polyacrylamide gel modification described in this embodiment is different from Example 1 in that: in step (3), gold nanoparticle solution, 30% stock gel solution , 1.5mol / L of 3-hydroxyaminomethane hydrochloric acid buffer (pH=8.8), 10% sodium lauryl sulfate solution, 10% ammonium persulfate solution and tetramethylethylenediamine solution according to volume ratio 2.3: 5 : 2.5: 0.1: 0.1: 0.004 mixed to prepare gel solution I; in step (5), gold nanoparticle solution, 30% stock gel solution, 1mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH = 6.8), 10% sodium lauryl sulfate solution, 10% ammonium persulfate solution and tetramethylethylenediamine solution are mixed according to volume ratio 1.4: 0.33: 0.25: 0.02: 0.02: 0.02 to obtain gel solution II ; A gold nanoparticle-based gradient polyacrylamide gel-modified sensor was prepared.

[0066] The 30% reserve gel solution was purchased from ...

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Abstract

The invention discloses an electrochemical sensor capable of simultaneously detecting glucose and uric acid, and belongs to the technical field of detection. Through pretreatment of the sensor electrode, the surface of the sensor electrode is rich in-OH groups, and the cleaning and activating capability of the electrode is improved. In the modification material of the sensor electrode, the gold nanoparticles have large specific surface area and excellent conductivity and catalytic ability, the catalytic ability to glucose or uric acid is enhanced, the gradient polyacrylamide gel realizes enrichment and separation of the to-be-detected substance glucose or uric acid, quantitative detection of a single component can be realized, and the specific surface area of the to-be-detected substance glucose or uric acid is increased. The sensor test paper can be used for simultaneously detecting two components, solves the technical problem that commercial sensor test paper cannot be used universally, provides more thoughts for medical diagnosis methods, and has a very wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, in particular to a non-enzyme electrochemical sensor that can be used to simultaneously detect glucose and uric acid. Background technique [0002] With the development of society and economy, people's living standards and dietary structure changes, the prevalence of diabetes and hyperuricemia is increasing year by year, and showing a younger trend, has become the world's largest and second largest metabolic chronic disease , and China is one of the countries with the largest number of patients and the highest incidence rate, which brings a certain burden and impact on social economy and personal life. [0003] Diabetes is a metabolic disease characterized by high blood sugar. Long-term high blood sugar can cause chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels, and nerves. Therefore, as the only standard for clinically diagnosing diabetes, the detection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/36G01N27/327G01N27/48
CPCG01N27/3278G01N27/36G01N27/48
Inventor 张帼威封顺石海珠陈诚张华菊
Owner SOUTHWEST JIAOTONG UNIV
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