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A method for in vitro regeneration of quinoa cotyledon nodes

An in vitro regeneration and wheat technology, applied in the field of plant tissue culture and biology, can solve the problems of inseparable and lack of high-efficiency in vitro regeneration system of quinoa, and achieve the goals of reducing damage, increasing callus induction rate, and increasing survival rate Effect

Active Publication Date: 2022-07-26
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of modern molecular technology to cultivate new varieties is inseparable from an efficient and stable genetic transformation system
The key factor restricting the genetic transformation of quinoa is the lack of an efficient in vitro regeneration system for quinoa

Method used

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  • A method for in vitro regeneration of quinoa cotyledon nodes
  • A method for in vitro regeneration of quinoa cotyledon nodes
  • A method for in vitro regeneration of quinoa cotyledon nodes

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Embodiment 1

[0033] (1) Seed disinfection: Rinse the plump quinoa seeds with sterile water, treat with 5‰ potassium permanganate solution for 3 minutes, then treat with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 5 times, and dry the material with filter paper surface moisture, inoculated into MS basic medium, and obtained sterile seedlings after culturing for 5 days ( figure 1 ). The amount of sucrose contained in the medium was 30g / L, the amount of agar powder contained was 5.5g / L, and the pH of the medium was adjusted to 5.8; the culture conditions were 25±2°C, the light intensity was 2000-3000lx, and the light was 12h / L. d.

[0034](2) Pre-cultivation of explants: the sterile seedlings in step (1) are cut with a scalpel to cut the cotyledon nodes of about 2 mm, and the existing bud spots are erased with a scalpel, and then placed in the pre-culture medium and cultured for three days. , the pre-medium was MS as the basic medium, and 6-benzylaminopuri...

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Abstract

The invention discloses a high-efficiency method for in vitro regeneration of quinoa cotyledon nodes. Using quinoa cotyledon nodes as the starting material, a large number of adventitious buds and regenerated plants can be obtained after induction, which provides technical support for quinoa transgenic. The method for in vitro regeneration of quinoa cotyledon nodes established by the invention provides technical support for the creation of new quinoa varieties, molecular mechanism research and the like, and promotes the development of the quinoa industry.

Description

technical field [0001] The invention relates to the field of plant tissue culture and biotechnology, in particular to a high-efficiency in vitro regeneration method of quinoa cotyledon nodes. Background technique [0002] Quinoa (C.quinoa Willd, 2n=36) belongs to the genus Amaranthaceae, an annual herb, a pseudo-cereal crop with grain as food (Giusti L.Elgenero Chenopodium en Argentina 1:Numeros decromosomas.Darwiniana, 1970, 16:98 –105.), the center of origin is the Titicaca Lake region of the Peruvian and Bolivian Andes. Quinoa, known as the "mother of grains", has rich and comprehensive nutrients, high protein content, and functional nutrients such as saponins. It is regarded as the "gold" in food. (BURNOUF-RADOSEVICH M, DELFEL N E, ENGLAND R. Gas chromatography mass spectrometry of oleanane and ursanetypetriterpenes application to Chenopodium quinoatriterpenes [J]. Phytochemistry, 1985, 24(9): 2063-2066.) In addition, quinoa showed outstanding resistance to Cold and sa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱木兰刘洁玉朱健康郑珂媛
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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