Electrochemical sensor for detecting 17 beta-estradiol as well as preparation and use methods thereof

An electrochemical and estradiol technology, applied in the field of sensors, can solve the problems of increasing the risk of ovarian cancer and breast cancer, female fertility problems, etc., and achieve the effect of excellent conductivity, high sensitivity, and large current response

Active Publication Date: 2021-06-25
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excess E2 left in animal foods such as meat and milk can cause fertility problems in women and increase the risk of ovarian and breast cancer

Method used

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  • Electrochemical sensor for detecting 17 beta-estradiol as well as preparation and use methods thereof
  • Electrochemical sensor for detecting 17 beta-estradiol as well as preparation and use methods thereof
  • Electrochemical sensor for detecting 17 beta-estradiol as well as preparation and use methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of a portable highly sensitive electrochemical aptasensor for detecting 17β-estradiol, the steps are as follows:

[0043] (1) Preparation of probes

[0044] 1. Preparation of AF1-ADA probe

[0045] Dissolve 0.5 g of adamantane carboxylic acid in methanol, treat it with a solution of sodium methoxide / methanol at 25% (w / w) until precipitation occurs, wash with low-temperature methanol several times, and dry in vacuo to obtain water-soluble sodium salt of adamantane carboxylic acid . A solution containing 0.2 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 100 µL of 0.1 M N-hydroxysuccinimide (NHS) and 500 µL of adamantanecarboxylate A mixed solution of acid sodium salt was added to 1 OD AF1. The solution was shaken overnight, the resulting mixture was centrifuged and washed several times, and the final AF1-ADA was dispersed in 600 µL of PBS (pH 7.4) solution. The sequence that recognizes E2 nucleic acid aptamer fragment 1 i...

Embodiment 2

[0056] A preparation method of a portable highly sensitive electrochemical aptasensor for detecting 17β-estradiol, the steps are as follows:

[0057] (1) Preparation of probes

[0058] a. Preparation of AF1-ADA probe

[0059] Dissolve 0.5 g of adamantane carboxylic acid in methanol, treat it with a solution of sodium methoxide / methanol at 25% (w / w) until precipitation occurs, wash with low-temperature methanol several times, and dry in vacuo to obtain water-soluble sodium salt of adamantane carboxylic acid . Add the mixed solution containing 0.2 g EDC, 100 µL 0.1 M NHS, and 500 µL adamantane carboxylic acid sodium salt to 1 OD AF1. The solution was shaken overnight, the resulting mixture was centrifuged and washed several times, and the final AF1-ADA was dispersed in 600 µL of PBS (pH 7.4) solution. The sequence that recognizes E2 nucleic acid aptamer fragment 1 is AF1: 5'-NH 2 -AAG GGA TGC CGT TTG GG-3';

[0060] b. Preparation of AF2-AuNPs probe

[0061] 100 mL of 0.01...

Embodiment 3

[0069] A preparation method of a portable highly sensitive electrochemical aptasensor for detecting 17β-estradiol, the steps are as follows:

[0070] (1) Preparation of probes

[0071] a. Preparation of AF1-ADA probe

[0072] Dissolve 0.5 g of adamantane carboxylic acid in methanol, treat it with a solution of sodium methoxide / methanol at 25% (w / w) until precipitation occurs, wash with low-temperature methanol several times, and dry in vacuo to obtain water-soluble sodium salt of adamantane carboxylic acid . Add the mixed solution containing 0.2 g EDC, 100 µL 0.1 M NHS, and 500 µL adamantane carboxylic acid sodium salt to 1 OD AF1. The solution was shaken overnight, the resulting mixture was centrifuged and washed several times, and the final AF1-ADA was dispersed in 600 µL of PBS (pH 7.4) solution. The sequence that recognizes E2 nucleic acid aptamer fragment 1 is AF1: 5'-NH 2 -AAG GGA TGC CGT TTG GG-3';

[0073] b. Preparation of AF2-AuNPs probe

[0074] 100 mL of 0.01...

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Abstract

The invention belongs to the field of sensors, and relates to a polymer film modified electrode and an aptamer sensor, in particular to an electrochemical aptamer sensor for detecting 17 beta-estradiol as well as a preparation method and a use method of the electrochemical aptamer sensor. Nucleic acid aptamer fragments AF1 and AF2 are introduced, when a target object E2 exists, AF1 and AF2 are combined on the surface of an electrode to form a compound, the function of a complete aptamer is shown, and the distance between gold nanoparticles and the surface of the electrode is changed before and after target molecules are recognized, so that the change of an electrochemical signal is obtained. The detection limit of the sensor can be 20 fM (LSD) and 0.77 fM (GCE). The design scheme of the sensor is constructed on a disposable, portable and low-cost LSG, so that commercialization of the sensor is facilitated, and the sensor has huge application potential in the aspect of realizing portable sensing equipment for detecting E2.

Description

technical field [0001] The invention belongs to the field of sensors, and relates to a polymer film modified electrode and an aptamer sensor, in particular to an electrochemical aptamer sensor for detecting 17β-estradiol and a preparation and use method thereof. Background technique [0002] 17β-estradiol (E2) is a common endocrine disrupting sterol hormone. The main estrogen in most mammals is E2. Other important estrogens include estriol and estrone. E2 has been widely used in animal husbandry to promote animal growth, increase lean meat percentage and milk production. Excess E2 left in animal foods such as meat and milk can cause fertility problems in women and increase the risk of ovarian and breast cancer. Abnormal levels of this hormone in the body can also lead to other health problems, such as weak bones, urinary tract infections, and even depression. Therefore, it is of great significance to establish a simple, rapid and sensitive E2 detection method. [0003] Ap...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3275G01N27/3277G01N27/48
Inventor 常竹刘瑾瑾朱碧澄朱旭郝远强张银堂徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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