QAMS (quantitative analysis of multiple components by single marker) method for determining content of six gingerol components in ginger juice brown sugar

Abstract

The invention provides a QAMS (quantitative analysis of multiple components by single marker) method for determining the content of six gingerol components in ginger juice brown sugar. The QAMS method is based on a high performance liquid chromatography, ECOSIL C18-eco100A C18 (4.6 * 250 mm, 5 [mu] m) is used as a chromatographic column, an acetonitrile-1% acetic acid solution is used as a mobile phase, gradient elution is performed, the flow velocity is 1.0 ml / min, the column temperature is 35 DEG C, and the detection wavelength is 280 nm. Results show that in a linear range, relative correction factors of synthesized capsaicin and 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol and 10-shogaol are respectively 0.9433, 1.6183, 1.2666, 1.3006, 1.0369 and 1.2355, the relative correction factors have good reproducibility under different experimental conditions, and calculated values and measured values of the six components have no significant difference. Therefore, the results prove that the method for determining the components of 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol and 10-shogaol in the ginger juice brown sugar is accurate and feasible, and the method can be used for quality control of the ginger juice brown sugar.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a one-measurement-multiple-evaluation method for determining the contents of six gingerols in ginger brown sugar. Background technique [0002] Ginger juice brown sugar is a sugar made from brown sugar, adding ginger juice and ginger powder. Traditional Chinese medicine nutrition believes that warm brown sugar exerts the effect of nourishing blood by "warming it, warming it, and dispersing it", while ginger has the functions of relieving the exterior and dispelling cold, warming the middle and relieving vomiting, warming the lungs and relieving cough and detoxification. , Commonly used for wind-cold cold, spleen-stomach-cold syndrome, stomach-cold vomiting, lung-cold cough, and detoxification of fish and crabs. There is a folk saying that "men can't live without ginger for a hundred days, and women can't live without sugar for a hundred days". Ginger soup made with...

AI Technical Summary

Problems solved by technology

There are 6 common gingerol components. The current 2020 Pharmacopoeia only stipulates 6-gingerol, 8-gingerol, and 10-gingerol as index components for ginger quality evaluation, which is not comprehensive enough

GB / T22293-2008 uses the correction factor method to measure 6 components, but the peak positioning is very vague, the separation is not ideal, the interference is large, and the durability is not clear

[0005] However, there is no bibliographical report on the main active ingredients of gingerol and gingerol as the quality evaluation index of ginger brown sugar.

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