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Method for extracting bound polyphenol from rape

A technology of rapeseed moss and extraction time, which is applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., to achieve the effect of simple separation operation process, improved yield and purity, and easy labor

Pending Publication Date: 2021-06-29
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no comprehensive research, development and utilization of the published literature at home and abroad, so it is of certain significance to extract the bound polyphenols from rape moss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100g of rapeseed moss to freeze-dry and pulverize to below 40 meshes. Under the conditions of 80% acetone concentration, 30mL / g liquid-to-solid ratio, 30min extraction time, and 210w ultrasonic power, the extraction is repeated three times, and the residue is separated by suction filtration; The filter residue was washed 3 times with methanol and 3 times with distilled water, freeze-dried and then crushed into fine powder with a pulverizer to pass through a 40-mesh sieve. Use 4 mol / L NaOH solution to heat the freeze-dried powder of the filter residue in a water bath at 85°C for 10 h at a solid-liquid ratio of 1:20 (g / mL), then use 6 mol / L hydrochloric acid to adjust the pH value of the hydrolyzed solution to 2, and filter with suction Separate the supernatant. Use an equal volume of ethyl acetate (volume ratio 1:1) to extract the supernatant, repeat the extraction three times, combine the extracts and evaporate at 40°C, and finally freeze-dry the concentrate to obt...

Embodiment 2

[0024] Take 100g of rapeseed moss to freeze-dry and pulverize to below 40 meshes. Under the conditions of 80% acetone concentration, 30mL / g liquid-to-solid ratio, 30min extraction time, and 210w ultrasonic power, the extraction is repeated three times, and the residue is separated by suction filtration; The filter residue was washed 3 times with methanol and 3 times with distilled water, freeze-dried and then crushed into fine powder with a pulverizer to pass through a 40-mesh sieve. Use 2 mol / L NaOH solution to heat the freeze-dried powder of the filter residue in a water bath at 95°C for 10 h at a solid-liquid ratio of 1:20 (g / mL), then use 6 mol / L hydrochloric acid to adjust the pH value of the hydrolyzed solution to 2, and filter with suction Separate the supernatant. Use an equal volume of ethyl acetate (volume ratio 1:1) to extract the supernatant, repeat the extraction three times, combine the extracts and evaporate at 40°C, and finally freeze-dry the concentrate to obt...

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PUM

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Abstract

The invention relates to a process technology for extracting bound polyphenol from rape, which comprises the following steps: carrying out ultrasonic treatment on rape powder by using 80% acetone, filtering to obtain filter residues, freeze-drying the filter residues, carrying out water bath by using a 2mol / L NaOH solution, adjusting the pH value of the solution to 2 by using 6mol / L hydrochloric acid, extracting the filtered filtrate by using ethyl acetate, concentrating the extract liquor, and freeze-drying to obtain the bound polyphenol of the rape. According to the process, the yield and purity of the bound polyphenol are greatly improved, the whole separation process has low environmental requirements and highest purity, the separation materials are cheap and easy to obtain, the separation operation process is simple, the manual control is easy, and the purification efficiency is high.

Description

[0001] Technical field [0002] The invention relates to a process for extracting bound polyphenols from rapeseed moss, which belongs to the technical field of plant extraction. Background technique [0003] Rapeseed is a cruciferous plant, the tender stems and leaves of rapeseed, also known as brassica, cold vegetable, mustard, bitter vegetable, moss mustard, cool in nature, sweet in taste, enters the liver, spleen, and lung meridian, has the functions of promoting blood circulation and removing blood stasis, detoxification Reduce swelling, widen the intestines and relieve constipation, strengthen the body and other effects. The therapeutic effect of rapeseed moss is to lower blood fat, detoxify and reduce swelling, widen intestines and laxatives, and strengthen the body. [0004] Bound polyphenols mainly refer to some hydrated tannins and proanthocyanidins bound to the cell wall, which are usually separated by chemical or enzymatic treatment to break the chemical bond betwe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61K135/00A61K127/00
CPCA61K36/31A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 胡璐曼谢文杰骆丽如唐忠海
Owner HUNAN AGRICULTURAL UNIV