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Cr (VI) stress resistant petroleum contaminated soil immobilized bioremediation technology

A technology for contaminated soil, bioremediation, applied in the restoration of contaminated soil, microorganisms, microorganism-based methods, etc., can solve the problems of reduced bioremediation efficiency, single, inorganic pollution, etc.

Active Publication Date: 2021-06-29
CHONGQING INST OF GEOLOGY & MINERAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, bioremediation technologies are often aimed at the remediation of soil contaminated by heavy metals or petroleum, but there are few technologies for the remediation of soil contaminated by heavy metals and petroleum at the same time.
However, in addition to serious petroleum pollutants, there are often inorganic pollution mainly heavy metals in polluted soil. As a result, in the bioremediation of petroleum-contaminated soil, petroleum-degrading bacteria are inhibited by heavy metals, and the bioremediation efficiency is remarkable. reduce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example is the research and development of soil immobilization petroleum-contaminated soil remediation technology against heavy metal Cr(Ⅵ) stress for the petroleum hydrocarbon-contaminated soil inside the Changning shale gas well site in Sichuan.

[0035] Using tartary buckwheat husks as raw materials, the tartary buckwheat husks were washed with tap water and purified water successively, dried at 105°C for 12 hours, the dried raw materials were crushed, and passed through a 10-mesh sieve. Gradually strengthen the reducing ability of biochar by the following four methods. First, take the pulverized tartary buckwheat shell and put it in the reaction kettle. Thermally carbonize for 8 hours, filter to obtain biochar, and wash the biochar with hydrochloric acid with a volume fraction of 10%. Second, place the cleaned biochar in another reactor filled with 500mL of ascorbic acid solution with a volume fraction of 50%, and conduct the second hydrothermal carbonization a...

Embodiment 2

[0039] This example is the research and development of soil immobilization oil-contaminated soil remediation technology for oil-contaminated soil in Tarim Oilfield, Xinjiang, which is resistant to heavy metal Cr(Ⅵ) stress.

[0040] Using tartary buckwheat shells as raw materials, wash the tartary buckwheat shells with pure water, dry at 105°C for 8 hours, crush the dried raw materials, and pass through a 10-mesh sieve. Put the pulverized tartary buckwheat shells in a 1L reactor, and add 1000mL of ethylenediamine aqueous solution with a mass fraction of 30% into the reactor for carbonization for 12 hours. The carbonization conditions are 300°C and 7000kPa. Wash the carbonized biochar with hydrochloric acid with a volume fraction of 12%, and dredge the internal pores of the biochar. The cleaned biochar was placed in another reactor filled with 1000mL of ascorbic acid solution with a volume fraction of 60% for the second hydrothermal carbonization. The carbonization conditions we...

Embodiment 3

[0044] This example is the research and development of the soil immobilization petroleum-contaminated soil remediation technology against the stress of heavy metal Cr(VI) for the petroleum-contaminated soil in Changqing Oilfield.

[0045] Using tartary buckwheat shells as raw materials, wash tartary buckwheat shells with tap water and pure water in turn, dry at 105°C for 8 hours, crush the dried raw materials, pass through a 10-mesh sieve, take 40g of crushed tartary buckwheat shells and put them in the reaction kettle At the same time, 500mL of ethylenediamine aqueous solution with a mass fraction of 24% was added, hydrothermally carbonized at 290°C and 6000kPa for 10h, the biochar was obtained by filtration, and the biochar was washed with hydrochloric acid with a volume fraction of 10%, and the cleaned biochar was Place it in another reactor filled with 500mL of ascorbic acid solution with a volume fraction of 55%, and carry out the second hydrothermal carbonization under th...

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PUM

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Abstract

The invention discloses a Cr (VI) stress resistant petroleum contaminated soil immobilized bioremediation technology. The method comprises the following steps of: by taking tartary buckwheat shells as a raw material, carrying out hydrothermal carbonization in an ethylenediamine aqueous solution and a ascorbic acid solution, carrying out rotary heating in formic acid and loading nano zero-valent aluminum, so as to gradually improve the reducibility of biochar, thereby realizing the reduction of Cr (VI); and loading glutamylcysteine synthetase and glutathione synthetase to enhance the detoxification capability of microorganisms. The method is very effective for bioremediation of petroleum contaminated soil stressed by heavy metal Cr (VI), and the heavy metal Cr (VI) in the soil is partially removed while petroleum is removed.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration and the field of microorganism immobilization, and in particular relates to a bioremediation technology for immobilization of petroleum-contaminated soil resistant to Cr(VI) stress. Background technique [0002] With the expansion of the national oil and gas energy development scale, the soil environment has been greatly polluted in the process of oil and gas energy exploitation, processing, transportation and storage. Pollutants destroy the cell membranes of soil microorganisms and plants, and inhibit soil enzyme activity. , and even enter the food chain and enrich the human body, causing extremely serious harm to human health. Bioremediation is widely used in petroleum-contaminated soil remediation because of its simplicity, convenience, low cost, and no secondary pollution. CN110003913A loads magnesium oxide on the surface and interlayer of montmorillonite, and mixes it with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N11/14C12R1/40
CPCB09C1/10C12N11/14
Inventor 任宏洋邓源鹏魏紫静龚星明侯迪雅孙雷涛王兵
Owner CHONGQING INST OF GEOLOGY & MINERAL RESOURCES
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