Preparation method of metal organic framework material for treating cerebral arterial thrombosis

A metal organic framework, ischemic stroke technology, applied in the field of biomedical materials, can solve problems such as inability to treat diseases, and achieve the effects of reducing nerve cell death rate, improving the microenvironment of the lesion, and reducing the infarct volume

Active Publication Date: 2021-07-02
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for preparing a metal-organic framework material for the treatment of ischemic stroke, so as to solve the problem in the prior art of researching and treating stroke in the pathological process of local brain tissue necrosis of ischemic stroke, The problem of not being able to treat the disease from the internal factors that cause the onset of stroke
[0009] In order

Method used

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  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis
  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis
  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis

Examples

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Example Embodiment

[0043] Example 1

[0044] Preparation method of metal organic frame material for ischemic stroke treatment, including the following steps:

[0045] Step 1, prepare metal organic frame materials;

[0046] Step 2, remove raw materials and solvent molecules in the metal organic frame material, activated metal organic frame material;

[0047] Step 3: Dopamine modification of the obtained metal organic frame material.

[0048] In the present embodiment, the prepared metal organic frame material is specifically:

[0049] Step 11, chloride (MnCl) 2 ), Organic ligand hexamethylene triphenyl (HATP) is mixed in deionized water;

[0050] In step 12, triethylamine is added dropwise to the liquid prepared in step 12, and then heat it to heat the reflux at 80 ° C for 24 h;

[0051] Step 13, the liquid obtained by the liquid obtained in step 12 is discarded, and the resulting black powder solid is a metal organic frame material.

[0052] In this embodiment, the raw material and solvent molecules...

Example Embodiment

[0069] Example 2

[0070] An application of metal organic frame material prepared by the preparation method of the metal organic frame material described above is in the treatment of ischemic stroke treatment, specifically:

[0071] 1. Polypineamine modified organometallic frame material (PDOPA-MOFS) diluted with PBS to a concentration of 12.5 micrograms / ml.

[0072] 2, C57 mice after anesthesia separating the right skin on the head, before the origin is the origin, 0.5 mm in the tail side direction, parallel to the right side of the middle sewing, 2 craniotic drilling;

[0073] 3. Separate the skin of the mouse neck, free to the right neck, introduced obstruction model dedicated lines by the neck arterial, starting at the designated position after 1 hour countdown;

[0074] 4. After the start timing, immediately use the Hamilton needle to perform a treatment liquid with a treatment liquid having a depth of 3 mm in the head drilling site, and the injection speed is 0.5 microlite...

Example Embodiment

[0076] Example 3

[0077] Such as figure 1 As shown, the present application adds the metal Mn to the organic ligand HATP to form a Mn-Hatp metal organic frame compound (Mn-Mo-MOF), and the surface of the Mn-MOF surface is modified by self-assembly.

[0078] Such as figure 2 As shown in vitro, PDOPA-MOFS antioxidant activity is detected using a superoxide dismutase (SOD) detection kit, i.e., the ability to remove free radicals such as superoxide anion and hydrogen peroxide. PDOPA-MOFS reflects PDOPA-MOFS by measuring the absorbance value corresponding to the detection system (solution) specific absorption wavelength by measuring the detection system (solution) Enzyme activity. Data proves that PDOPA-MOFS has a similar SOD enzyme activity in vitro to remove superoxide anions ( figure 2 ).

[0079] Among them, the SOD enzyme detection principle (WST) is: a hyperoxide anion in the kit system in the kit system. Optical oleva can cause WST-1 to produce a water-soluble methicular dye, a...

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Abstract

The invention discloses a preparation method of a metal organic framework material for treating cerebral arterial thrombosis. The preparation method comprises the following steps: step 1, preparing the metal organic framework material; step 2, removing unreacted raw materials and solvent molecules in the metal organic framework material, and activating the metal organic framework material; and step 3, carrying out dopamine modification on the obtained metal organic framework material. The metal organic framework material has superoxide dismutase (SOD), oxygen free radicals can be effectively converted into water and oxygen, meanwhile, the novel metal organic framework material can up-regulate the activity of endogenous antioxidant enzyme of nerve cells, and free radicals are further removed. The metal organic framework material is delivered in the stroke mouse in a lateral ventricle injection mode, the focus microenvironment is improved, the nerve cell death rate is reduced, the infarct volume is reduced, and the mouse postoperative behavioral function is effectively recovered.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of a metal organic framework material used for the treatment of ischemic stroke. Background technique [0002] Stroke is an acute cerebrovascular disease. According to the pathological characteristics, stroke is mainly divided into ischemic stroke and hemorrhagic stroke. Ischemic stroke accounts for more than 80% of the whole. Free radicals play an important role in the pathological process of local brain tissue necrosis in ischemic stroke. Due to the obstruction of the blood flow supply, the local nerve cells lack energy and oxygen, a large amount of calcium ions in the nerve cells flow in, and the mitochondrial function is damaged, causing excessive free radicals, such as superoxide anion (O2-), hydrogen peroxide ( H2O2), hydroxyl radicals (.OH) and reactive nitrogen (ONOO·), which will further damage nerve cells, causing cell apoptosis and ne...

Claims

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Application Information

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IPC IPC(8): C08G83/00A61P9/10
CPCC08G83/008A61P9/10
Inventor 王琳王征王健苏强飞汪洋夏克尔扎提肖哈拉提陈冲
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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