Induction culture medium for tissue culture and rapid propagation of dracocephalum plants and application of induction culture medium

Abstract

The invention relates to the technical field of plant tissue culture, and discloses a tissue culture and rapid propagation method for dracocephalum plants and application thereof. The tissue culture and rapid propagation method for the dracocephalum plants comprises the steps of sterile explant acquisition, induction culture, differentiation culture, rooting culture and acclimatization and transplantation, wherein the induction culture specifically comprises the steps of taking 0.5-2.0 cm of a sterile explant, inoculating the sterile explant into an induction culture medium, and culturing for 7-15 days to obtain a callus; the temperature of the induction culture is 24-28 DEG C, the illumination time is 8-12 h / d, and the illumination intensity is 2000-4000 Lx; the induction culture medium comprises 0.8 to 1.5 mg / L of an MS culture medium, 0.5 to 1.0 mg / L of 6-BA, 0.1 to 0.3 mg / L of IAA, 0.1 to 0.2 mg / L of 2, 4-D, 25 to 30 g / L of sucrose, 5 to 7 g / L of agar, 0.2 to 0.4 mg / L of Vc and 2.0 to 6.0 mg / L of sodium nitroprusside, and the pH value of the induction culture medium is 5.6 to 6.2. The invention also discloses an application of the tissue culture and rapid propagation method for the dracocephalum plants, and the tissue culture and rapid propagation method achieves the effect of efficiently breeding cultivated seedlings of the dracocephalum plants through the targeted culture steps, multi-plant hormone combined culture medium components and culture conditions.

Description

[0001] This application is a divisional application. The application number of the original application is: 2019110078170. The application name is: A method for tissue culture and rapid propagation of plants of the genus Qinglan and its application. The application date is: October 22, 2019 technical field [0002] The invention relates to the technical field of plant tissue culture, in particular to an induction medium for tissue culture and rapid propagation of plants of the genus Cymbidium and its application. Background technique [0003] Dracocephalum belongs to Labiatae, herbaceous, perennial, with woody rhizomes, and rarely annual. Stems often arise from rhizomes, erect, sparsely spread, often unbranched or with few branches, sparsely numerous branches, quadrangular. Leaves opposite, basal leaves with long stalks, cauline leaves with short stalks or sessiles, often heart-shaped ovate or oblong, or lanceolate, margins crenate or serrate, or entire, usually not Divided...

AI Technical Summary

Problems solved by technology

[0005] However, at present, when conventional plant tissue culture methods and common plant hormone ratios are used to breed the plants of the genus Orchid, the efficiency is very low, even not as high as the seed propagation efficiency

In addition, due to the small size of Maojiancao seeds, it is not easy to collect, and the seed germination rate is not high in the natural environment, which makes it difficult to reproduce the seeds; at the same time, because of its rich secondary metabolites, it contains a variety of enzymes and various small Molecular substances have seriously affected the effect of the phytohormone combination of conventional plant tissue culture, so that the conventional plant tissue culture method can not effectively achieve the efficiency of tissue culture for the breeding of Maojiancao

For example, the commonly used medium for tobacco tissue culture cannot complete the regeneration of plants of the genus Chrysanthemum, such as Minshan Maojiancao, and its induction medium cannot induce enough callus, and the browning of tissue materials is also obvious during the induction process.

Images