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Artificial corneal endothelium in corneal stroma and preparation method thereof

A technology of artificial cornea and corneal stroma, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of artificial cornea prolapse, wound leakage, corneal edema relying on donated cornea, etc., and achieve good comfort, medium water content, The effect of good market prospects

Pending Publication Date: 2021-07-13
广州宏达医疗设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In view of this situation, the purpose of the present invention is to provide an artificial corneal endothelium in the corneal stroma and its preparation method to solve the problem that the treatment of corneal edema caused by corneal endothelial lesions is highly dependent on donated corneas, while also avoiding the need for other artificial corneal implants. Wound leakage and prolapse of artificial cornea after implantation

Method used

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  • Artificial corneal endothelium in corneal stroma and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method for artificial corneal endothelium in corneal stroma, comprising the steps of:

[0034] (1) Preparation of dry silicon hydrogel mold base: the specific steps are as follows:

[0035] S1. Weigh each raw material according to the following mass percentages: 32% of silicon-containing monomer, 44% of hydrophilic monomer, 0.43% of crosslinking agent, 0.26% of photo / thermal initiator, and the rest is solvent;

[0036] S2. After mixing and stirring each component according to the formula amount, pour it into the mold, and polymerize under the conditions of conventional heating or ultraviolet lamp irradiation;

[0037] S3. Place the mold in an alcoholic solvent with a volume fraction of 20% for separation, and collect the silicon hydrogel mold base;

[0038] S4. Place the mold base in isopropanol with a volume fraction of 15% and reform it for 1 hour; isopropanol is used as a cleaning and degreasing agent, which can completely clean the grease material on ...

Embodiment 2

[0046] A preparation method for artificial corneal endothelium in corneal stroma, comprising the steps of:

[0047] (1) Preparation of dry silicon hydrogel mold base: the specific steps are as follows:

[0048] S1. Weigh each raw material according to the following mass percentages: 23% of silicon-containing monomer, 36% of hydrophilic monomer, 0.05% of crosslinking agent, 0.05% of photo / thermal initiator, and the rest is solvent;

[0049] S2. After mixing and stirring each component according to the formula amount, pour it into the mold, and polymerize under the conditions of conventional heating or ultraviolet lamp irradiation;

[0050] S3. The mold is placed in an alcohol solvent with a volume fraction of 10% for separation, and the silicon hydrogel mold base is collected;

[0051] S4. Place the mold base in isopropanol with a volume fraction of 10% and reform it for 1 hour; isopropanol is used as a cleaning and degreasing agent, which can completely clean the grease materia...

Embodiment 3

[0059] A preparation method for artificial corneal endothelium in corneal stroma, comprising the steps of:

[0060] (1) Preparation of dry silicon hydrogel mold base: the specific steps are as follows:

[0061] S1. Weigh each raw material according to the following mass percentages: 40% silicon-containing monomer, 52% hydrophilic monomer, 8% crosslinking agent, 5% photo / thermal initiator, and the rest is solvent;

[0062] S2. After mixing and stirring each component according to the formula amount, pour it into the mold, and polymerize under the conditions of conventional heating or ultraviolet lamp irradiation;

[0063] S3. Place the mold in an alcohol solvent with a volume fraction of 30% for separation, and collect the silicon hydrogel mold base;

[0064] S4. Place the mold base in isopropanol with a volume fraction of 20% and reform it for 1 hour; isopropanol is used as a cleaning and degreasing agent, which can completely clean the grease material on the mold base and im...

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Abstract

The invention discloses an artificial corneal endothelium in corneal stroma and a preparation method thereof. The preparation method comprises the following steps: preparing a dry-state silicon hydrogel mold blank: polymerizing a silicon hydrogel monomer into the dry-state silicon hydrogel mold blank; turning processing: fixing the dry silicone hydrogel mold base to a numerical control lathe, and turning artificial corneal endothelium, wherein the curvature radius of the front surface of the artificial corneal endothelium is consistent with that of the front surface of the cornea, and the curvature radius of the rear surface of the artificial corneal endothelium is close to that of the rear surface of the cornea; and polishing and hydration: polishing and trimming the front and rear surfaces of the artificial corneal endothelium, and fully hydrating in normal saline to form the colloidal corneal endothelium. The bottleneck that corneal endothelium transplantation must depend on the cornea of a high-quality donor is broken through, and the artificial corneal endothelium is implanted between corneal stroma layers of a patient, so that the artificial cornea does not take off, the intraocular interference is avoided, and the product is very safe.

Description

technical field [0001] The invention relates to the technical field of artificial cornea, in particular to an artificial corneal endothelium in corneal stroma and a preparation method thereof. Background technique [0002] The cornea is a layer of transparent film located at the front of the eyeball, which is divided into epithelial cell layer, Bowman's layer, stroma layer, Bowman's layer and endothelial cell layer from outside to inside. The thickness of the central cornea is about 520-540 microns, and the thickness of the endothelial cell layer is about 10-20 microns. Corneal endothelial cells are a single layer of cells that play a vital role in maintaining the transparency of the cornea. Because the cornea is in direct contact with the external environment, it is extremely susceptible to mechanical trauma, thermal burns, chemical burns, and infection by pathogenic microorganisms. The cornea may also have congenital hereditary or developmental abnormalities, resulting in...

Claims

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Application Information

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IPC IPC(8): A61L27/16A61L27/50A61L27/52C08F220/54C08F230/08C08F226/10C08F220/58C08F283/12
CPCA61L27/16A61L27/50A61L27/52C08F220/54C08F283/122C08F230/085A61L2430/16C08L33/24C08L51/08C08L43/04C08F226/10C08F220/58
Inventor 柏英
Owner 广州宏达医疗设备有限公司
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