CsMYB110 gene and application thereof in regulation of synthesis of carotenoids

A technology of carotene and genes, applied in the field of genetic engineering, can solve problems such as inability to breed and provide excellent genetic resources

Active Publication Date: 2021-07-16
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

Although the release of tea genome data in recent years provides a theoretical reference for us to analyze the carotenoid synthesis mechanism in tea tree leaves at the molecular l

Method used

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  • CsMYB110 gene and application thereof in regulation of synthesis of carotenoids
  • CsMYB110 gene and application thereof in regulation of synthesis of carotenoids
  • CsMYB110 gene and application thereof in regulation of synthesis of carotenoids

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Experimental program
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Embodiment 1

[0020] 1. Cloning and sequence structure analysis of CsMYB110 gene

[0021] CsMYB110 gene is a tea tree MYB class transcription factor gene, and its cloning and sequence structure analysis are as follows:

[0022] Shucha, a national-level tea tree variety, was first planted in the Agricultural Industrial Park of Anhui Agricultural University, Hefei, Luyang District, Anhui Province. The young leaves were used for RNA extraction. Total RNA was extracted using Trizol reagent (Invitrogen, USA) according to the instructions, and the RNA content and quality were detected with a spectrophotometer.

[0023] Reverse transcription to generate the first strand: take 1 μg RNA as a template, add 0.5 μg of downstream primers respectively according to the instructions of Promega’s M-MLV1 reverse transcriptase reagent kit, dilute to 15 μl, denature at 70°C for 5 minutes, and place on ice immediately Leave it for 5 minutes; then add M-MLV 5×buffer 5μl, dNTP (25mM) 5μl, rRNasin Ribonuclease In...

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Abstract

The invention belongs to the technical field of gene engineering, and discloses a CsMYB110 gene and an application thereof in regulation of synthesis of carotenoids. The nucleotide sequence of the CsMYB110 gene is as shown in SEQ ID NO. 1 in a sequence table. The amino acid sequence of protein coded by the CsMYB110 gene is as shown in SEQ ID NO. 2 in the sequence table. The expression mode of CsMYB110 is highly related to synthesis of the carotenoids in tea tree leaves, and the gene can significantly promote synthesis and accumulation of the carotenoids in plants. Cloning of the gene not only facilitates analysis of a regulation mechanism of the carotenoids in tea tree leaves, but also is good for cultivation of tea tree varieties with high carotenoid content and improvement of tea quality, and great application value is achieved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, specifically CsMYB110 gene and its application in regulating carotenoid synthesis. Background technique [0002] Tea tree (Camellia sinensis (L.) O.Kuntze) is an important economic crop in my country. The pigments in its leaves are mainly divided into two categories: fat-soluble pigments and water-soluble pigments, among which flavonoids, anthocyanins and theaflavins Chlorophyll, carotenoids, and proanthocyanidins are fat-soluble pigments. These pigments are closely related to the quality of tea, including the color of dry tea, tea soup and leaf bottom, and the formation of tea aroma. [0003] Carotenoids are widely found in leaves, flowers and fruits of higher plants. They are natural yellow, orange-red or red lipophilic molecules belonging to terpene pigments. Carotenoids in tea are between 36 and 73mg per 100g dry weight, mainly composed of β-carotene, lutein and zeaxanthin, which...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/00
CPCC07K14/415C12N15/825C12N15/8261
Inventor 李鹏辉富嘉敏赵剑徐玉婕
Owner ANHUI AGRICULTURAL UNIVERSITY
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