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Application of csmyb110 gene in regulation of carotenoid synthesis

A technology of carotene and genes, applied in the field of genetic engineering, can solve problems such as inability to breed and provide excellent genetic resources

Active Publication Date: 2022-03-18
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the release of tea genome data in recent years provides a theoretical reference for us to analyze the carotenoid synthesis mechanism in tea tree leaves at the molecular level, there are few studies on the synthesis of tea tree carotenoids at the gene level, which cannot provide a basis for genetic engineering breeding. excellent genetic resources

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  • Application of csmyb110 gene in regulation of carotenoid synthesis
  • Application of csmyb110 gene in regulation of carotenoid synthesis
  • Application of csmyb110 gene in regulation of carotenoid synthesis

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Embodiment 1

[0020] 1. Cloning and sequence structure analysis of CsMYB110 gene

[0021] CsMYB110 gene is a tea tree MYB class transcription factor gene, and its cloning and sequence structure analysis are as follows:

[0022] Shucha, a national-level tea tree variety, was first planted in the Agricultural Industrial Park of Anhui Agricultural University, Hefei, Luyang District, Anhui Province. The young leaves were used for RNA extraction. Total RNA was extracted using Trizol reagent (Invitrogen, USA) according to the instructions, and the RNA content and quality were detected with a spectrophotometer.

[0023] Reverse transcription to generate the first strand: take 1 μg RNA as a template, add 0.5 μg of downstream primers respectively according to the instructions of Promega’s M-MLV1 reverse transcriptase reagent kit, dilute to 15 μl, denature at 70°C for 5 minutes, and place on ice immediately Leave it for 5 minutes; then add M-MLV 5×buffer 5μl, dNTP (25mM) 5μl, rRNasin Ribonuclease In...

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Abstract

The invention belongs to the technical field of genetic engineering and discloses the application of CsMYB110 gene in regulating carotenoid synthesis. The nucleotide sequence of the CsMYB110 gene is shown in SEQ ID NO.1 in the sequence listing; the amino acid sequence of the protein encoded by the CsMYB110 gene is shown in SEQ ID NO.2 in the sequence listing. The expression pattern of CsMYB110 is highly correlated with carotenoid synthesis in tea leaves, and this gene can significantly promote the accumulation of carotenoid synthesis in plants. The cloning of the gene will not only help analyze the regulation mechanism of tea tree leaf carotenoids, but also help to cultivate tea tree varieties with higher carotenoid content and improve the quality of tea leaves, which has great application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of CsMYB110 gene in regulating carotenoid synthesis. Background technique [0002] Tea tree (Camellia sinensis (L.) O.Kuntze) is an important economic crop in my country. The pigments in its leaves are mainly divided into two categories: fat-soluble pigments and water-soluble pigments, among which flavonoids, anthocyanins and theaflavins Chlorophyll, carotenoids, and proanthocyanidins are fat-soluble pigments. These pigments are closely related to the quality of tea, including the color of dry tea, tea soup and leaf bottom, and the formation of tea aroma. [0003] Carotenoids are widely found in leaves, flowers and fruits of higher plants. They are natural yellow, orange-red or red lipophilic molecules belonging to terpene pigments. Carotenoids in tea are between 36 and 73mg per 100g dry weight, mainly composed of β-carotene, lutein and zeaxanthin, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/00
CPCC07K14/415C12N15/825C12N15/8261
Inventor 李鹏辉富嘉敏赵剑徐玉婕
Owner ANHUI AGRICULTURAL UNIVERSITY