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Detection card for simultaneously detecting aflatoxin B1 and zearalenone

A technology of zearalenone and aflatoxin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of expensive operation of instruments and equipment, difficulty in being suitable for ordinary personnel, and inconvenient detection, so as to ensure food safety, The effect of simple method and convenient operation

Inactive Publication Date: 2021-07-16
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, establishing a new detection method for aflatoxin B1 and zearalenone to quickly detect and screen contaminated feed is of great significance to human food safety. Mass spectrometry, high performance liquid chromatography and other detection methods are the main methods, but these detection technologies require expensive equipment and professional operations, which are difficult for ordinary people, and have poor flexibility and inconvenient detection
On the other hand, colloidal gold lateral flow chromatography technology is mainly used to qualitatively detect a single type of mycotoxin residue. This test card can meet the rapid requirements on the spot, but it can only be qualitative, and the sensitivity is low, and the naked eye observation is relatively subjective. , poor repeatability

Method used

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  • Detection card for simultaneously detecting aflatoxin B1 and zearalenone
  • Detection card for simultaneously detecting aflatoxin B1 and zearalenone
  • Detection card for simultaneously detecting aflatoxin B1 and zearalenone

Examples

Experimental program
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Effect test

preparation example Construction

[0053] Preparation of test card:

[0054] (1) Preparation of colloidal gold solution: take a 150mL triangular flask, treat it with aqua regia overnight, add 100mL ultrapure water, and then add 1% chloroauric acid (HAuCl 4 •3H 2 (0) 1mL, prepared into 100mL 0.01% chloroauric acid aqueous solution, magnetically heated and stirred until the solution boiled for 2min, then immediately added 0.8ml 2% trisodium citrate (Na 3 C 6 h 5 o 7 • 2H 2 (0) solution, continue to stir and heat, when the color of the solution turns into transparent purple red completely, keep the solution boiling for about 2min, stop heating, stir and cool at room temperature, store it for later use at 4°C, and pass the prepared colloidal gold solution through ultraviolet scanning and transmission Microscope for evaluation.

[0055] The evaluation method is to scan the colloidal gold with an ultraviolet spectrophotometer to obtain the maximum absorption peak wavelength and peak width, and observe the parti...

Embodiment 1

[0066] The feed was mixed with different qualities of aflatoxin B1 and zearalenone, and 6 kinds of toxin feeds and blank controls were prepared and numbered: the content of aflatoxin B1 in feeds numbered 1-3 was 50, 100, 200µg / kg, 5-7 contain zearalenone, the content is 100, 200, 500µg / kg in turn, and Nos. 4 and 8 are blank controls. Two kinds of toxin detection cards were used to detect 7 samples with the above detection method, and each sample was repeated 3 times. The results are shown in Table 1.

[0067] From the results, it can be seen that the recovery rate of aflatoxin B1 in feed samples by the test cards of the two toxins ranges from 92.0-117.0%, and the coefficient of variation is 2.25%-3.41%; the recovery rate range of zearalenone in feed samples is 93.4-106.7%, and the coefficient of variation is 2.69%-4.82%. In summary, the recovery rate variation range of the two toxin test cards is within 25%, and the variation coefficient is within 15%, indicating that the test...

Embodiment 2

[0071] Randomly select 10 types of market feeds, use two kinds of toxin detection cards, test 10 feed samples with the above detection method, set up a blank control, repeat 3 times for each, and compare the results with the HPLC test results (Table 2). The results show that the coincidence rate of the two is 100%, and when the aflatoxin B1 content in the feed is lower than 100 (µg / kg) and the zearalenone content is lower than 500 (µg / kg), the detection card of the present invention It has advantages in sensitivity.

[0072] Table 2 Comparison of two kinds of toxin test cards to determine feed results and instrument results

[0073]

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Abstract

The invention discloses preparation and application of a detection card for simultaneously detecting aflatoxin B1 and zearalenone, which belong to the technical field of agricultural product safety detection. The detection card comprises a sample pad, a colloidal gold membrane, a nitrocellulose membrane and a water absorption pad, wherein the colloidal gold membrane is coated with a detection antibody colloidal gold marker, and the nitrocellulose membrane is coated with a detection line T1 of an aflatoxin B1 antigen, a detection line T2 of a zearalenone antigen and a quality control line C of an IgG antibody. A to-be-detected solution flows to the water absorption pad from the sample pad, components of the to-be-detected solution are specifically enriched when passing through the detection line T1, the detection line T2 and the quality control line C, high-sensitivity real-time quantitative field detection is achieved in cooperation with a specific signal collection instrument, zearalenone and aflatoxin B1 in feed are rapidly detected at the same time, and the detection accuracy is high. The detection method is fast, simple, convenient, low in cost and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of agricultural product safety detection, and in particular relates to the preparation and application of a detection card for simultaneously detecting aflatoxin B1 and zearalenone. Background technique [0002] Zearalenone (ZEN) is mainly produced by Fusarium graminearum and Fusarium yellow. ZEN can mainly contaminate corn, wheat, barley, oats and other crops. After Plcinta investigated the mycotoxins in grains and animal feeds of nearly 20 countries in the world, it was found that most countries' grains and animal feeds were contaminated by ZEN to varying degrees. ZEN has a strong estrogen effect, which can cause hyperestrogenism and severe reproductive tract symptoms and infertility in pigs, rats, mice, poultry and other animals. It also has immunotoxicity and genotoxicity. Humans also have estrogen effects. [0003] Aflatoxin (AFT) is mainly a secondary metabolite produced by some specific molds (Aspe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 洪霞杜霞
Owner JIANGSU WISE SCI & TECH DEV
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