Compound in sabia parviflora leaves and preparation method and application of compound
A compound, a technology for clearing wind, applied in the field of pharmaceutical compounds, can solve problems such as unclear mechanism of action, and achieve the effects of improving body weight status, reducing inflammatory symptoms, and reducing synovial tissue hyperplasia
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Embodiment 1
[0080] Embodiment 1 preparation method
[0081] Take 16.7 kg of vine leaves of Xiaohua Qingfeng, extract twice with 10 times the amount of 70% ethanol under reflux, extract for 2 hours each time, combine the extracts, recover the ethanol extract under reduced pressure, add 10 L of distilled water to dissolve, then use petroleum ether, Ethyl acetate, water-saturated n-butanol extraction, extraction until the organic layer is nearly transparent, the organic solvent is recovered under reduced pressure to the extract, and the dried products after vacuum drying are petroleum ether layer, ethyl acetate layer, water-saturated n-butyl alcohol layer, respectively. For the alcohol layer, take 280g of the n-butanol layer extract, dissolve it in methanol, add 450g of silica gel and grind it evenly with a mortar, stir the sample evenly until it is completely absorbed, put it in a low-temperature oven to dry, and set aside, take 4kg of silica gel and mix it with CH 2 Cl 2 After stirring, w...
Embodiment 2
[0096] Embodiment 2 preparation method
[0097] Take 10 kg of vine leaves of Xiaohuaqingfeng, reflux extract with 8 times the amount of 60% ethanol twice, extract for 1 hour each time, combine the extracts, recover the ethanol extract under reduced pressure, add 8L of distilled water to dissolve, then use petroleum ether, acetic acid Ethyl ester, water-saturated n-butanol extraction, extraction until the organic layer is nearly transparent, the organic solvent is recovered under reduced pressure to the extract, and the dried products after vacuum drying are petroleum ether layer, ethyl acetate layer, water-saturated n-butanol layer, etc. Floor;
[0098] Take 260g of the n-butanol layer extract, dissolve it in methanol, add 400g of silica gel and use a mortar to evenly stir and grind the sample until it is completely adsorbed, place it in a low-temperature oven to dry, and set aside. Take 2kg of silica gel and mix it with CH 2 Cl 2 After stirring, wet packed the column, and l...
Embodiment 3
[0106] Embodiment 3 preparation method
[0107] Take 21 kg of vine leaves of Xiaohua Qingfeng, reflux extract with 12 times the amount of 80% ethanol for 4 times, extract for 3 hours each time, combine the extracts, recover the ethanol extract under reduced pressure, add 12L of distilled water to dissolve, and then use petroleum ether and acetic acid respectively Ethyl ester, water-saturated n-butanol extraction, extraction until the organic layer is nearly transparent, the organic solvent is recovered under reduced pressure to the extract, and the dried products after vacuum drying are petroleum ether layer, ethyl acetate layer, water-saturated n-butanol layer, etc. Floor;
[0108] Take 300g of the n-butanol layer extract, dissolve it in methanol, add 500g of silica gel and use a mortar to evenly stir and grind the sample until it is completely absorbed, place it in a low-temperature oven to dry, and set aside, take 6kg of silica gel and mix it with CH 2 Cl 2 After stirring...
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