Dunaliella salina non-destructive circulating culture device

Abstract

The invention provides a dunaliella salina non-destructive circulating culture device. The dunaliella salina non-destructive circulating culture device comprises a liquid level difference airlift reactor combining negative pressure and positive pressure and a photobioreactor communicated with the upper end and the lower end of the airlift reactor; bypass circulation is added to the airlift reactor, and the size or opening and closing of a valve can be independently controlled according to the concentration of dissolved oxygen in the device; and in addition, a carbon dioxide supplementing device is additionally arranged and is structurally separated from a dissolved oxygen analysis mechanism. A jet device is adopted to introduce disinfection compressed air, the rising of dunaliella salina liquid is promoted to realize non-destructive circulation; a carbon dioxide supplementing module and a dissolved oxygen analysis module are physically isolated, bypass circulation flow and circulation time can be adjusted by the bypass circulation according to the dissolved oxygen value in an exchange column, the utilization efficiency of carbon dioxide is improved, and further, photosynthesis of dunaliella salina cells is promoted, so that the biological yield is increased, and finally, the artificial controllability of brine culture of dunaliella salina is realized.

Description

technical field [0001] The invention relates to the technical field of salina cultivation devices, in particular to a salina non-damaging circulation cultivation device. Background technique [0002] Salina is a single-celled green alga with double flagella. The length of the flagella is 1-2 times the length of the cell, and it is extremely vulnerable to external mechanical damage. Its cell wall structure is mainly composed of glycoprotein complexes and lacks rigid cellulose components. This flexible cell structure also makes Salina cells extremely vulnerable to external mechanical shear damage and growth in the process of industrial cultivation. decline and even cell death. In addition, the damage and death of salina cells will release a large amount of organic matter into the culture system, which will promote the reproduction of bacteria and even harmful organisms; the organic matter such as protein dissolved in the system will generate a large number of air bubbles unde...

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Problems solved by technology

Cai Zhiwu's "A production device for industrialized cultivation of microalgae" (Chinese patent, CN201245640Y) discloses a closed photobioreactor model operated by air push, but this invention is only applicable to liquids flowing horizontally

Luo Guanghong et al.'s "a salina culture device" (Chinese patent, CN209722114 U) uses a motor to drive the stirring shaft and stirring fins to stir the salina culture solution to avoid cell attachment, but its mechanical rotation will still introduce external sources Mechanical shear force, which may cause damage to the cells; in addition, agitation increases the energy consumption of the equipment

"A self-absorbing oxygen tubular photobioreactor" (Chinese patent, CN107043693B) by He Yaling and others absorbs the high concentration of dissolved oxygen in the reactor by introducing a fuel cell stack, but still needs to use a circulating water pump to realize the algae liquid. cycle

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