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Method for analyzing a pyrrolizidine-based alkaloid

A pyrrolizidine and analysis method technology, applied in the analysis field of pyrrolizidine alkaloids, to achieve the effects of reducing time/labor, rapid qualitative and quantitative analysis, stable and accurate qualitative and quantitative analysis

Active Publication Date: 2021-07-23
SHIMADZU (CHINA) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0018] In the present invention, the following scheme is used to solve the technical problem of rapid and accurate qualitative and quantitative analysis of pyrrolizidine alkaloids

Method used

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  • Method for analyzing a pyrrolizidine-based alkaloid
  • Method for analyzing a pyrrolizidine-based alkaloid
  • Method for analyzing a pyrrolizidine-based alkaloid

Examples

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Embodiment

[0079] Examples of the present invention will be described below, but the present invention is not limited to the following examples.

[0080] In this example, a triple quadrupole mass spectrometer in series with a probe electrospray ion source was used to analyze the monocrotaline N-oxide, adonifrin base, inverted nectar base, and senecoferin in the Chinese herbal medicine Senecio and Aster Alkali, Sennecine Nine, Senecone Nine, Senecone N-oxide, Senecio Phenyl N-oxide, Kirschner's Senecone Base.

[0081] The configuration of the device and the conditions, steps and results of the detection are described in detail below.

[0082] In this example, the samples of Senecio and Aster were crushed respectively according to the following steps, extracted with methanol water, and then supplied to a triple quadrupole mass spectrometer in series with a probe electrospray ion source after dilution, and carried out by the internal standard method. analyze.

[0083] (1) Accurately weigh...

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Abstract

The present invention relates to a method for analyzing a pyrrolizidine-based alkaloid, which is a method for analyzing a pyrrolizidine-based alkaloid using probe electrospray ionization mass spectrometry on a sample to be analyzed, the method comprising: a dilution step for diluting the sample by adding a buffer solution to the sample; and a mass spectrometry step for subjecting the diluted sample to probe electrospray ionization and introducing into a mass spectrometry device, the buffer solution containing one or more substances selected from the group consisting of carboxylic acids having an acid dissociation constant (pKa) of 4.8 or less and ammonium salts of the carboxylic acids.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, mass spectrometry, and Chinese herbal medicine analysis, and in particular to an analysis method for pyrrolizidine alkaloids. Background technique [0002] Pyrrolizidine alkaloids (PAs), also known as double-condensed pyrrolidine alkaloids, are a class of widely distributed plant toxins. PAs have been detected in 13 families of plants, most of which belong to Boraginaceae (Commercialis and Heliotrope), Compositae (Senecio, Rugonia, Ligularia and Eupatoria) and Fabaceae. (Lilium genus). Most PAs are hepatotoxic, and some PAs are also carcinogenic, mutagenic, and teratogenic. [0003] As people's awareness of the toxicity of PAs continues to deepen, the prescribed intake of PAs is becoming more and more stringent. In 2014, the European Medicines Agency required the daily intake limit of PAs in short-term oral medicines from all sources to be 0.005 μg / kg (based on an average body weight of 65...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/68
CPCG01N27/68
Inventor 陈振贺李晓东滨田尚树
Owner SHIMADZU (CHINA) CO LTD
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