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Method for detecting oxalic acid by using quantum dots and application

A technology of quantum dots and quantum dot solution, which is applied in the field of chemical detection to achieve the effects of easy operation, mild reaction conditions and low cost

Active Publication Date: 2021-07-23
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no published patents and reports in this field using quantum dots to detect oxalic acid content in urine

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  • Method for detecting oxalic acid by using quantum dots and application
  • Method for detecting oxalic acid by using quantum dots and application
  • Method for detecting oxalic acid by using quantum dots and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Oxalic acid analysis

[0044] to the solution containing 5 µl CuSO 4 (e.g., 1-100 μmM) and 50 μl of MOPS buffer (100 mmol / L NaNO 3 , 2.5 mmol per liter Mg(NO 3 ) 2 ,pH 7.4) solution, add 40 μl of detection solution containing different concentrations of oxalic acid (for example, oxalic acid concentration is 0.1 μM-10mM), incubate at room temperature (for example, 1–5 minutes) to complete the reduction reaction. Subsequently, 1-100 microliters of quantum dots (CdTe and / or CdSe) were added to the above solution and incubated at room temperature (eg, 1-5 minutes). Finally, the above solution was diluted to 200 μl with high-purity water. Under the excitation of 365 nm light, the quantification of oxalic acid was realized by measuring the fluorescence spectrum in the range of 570-720 nm.

[0045] 2. Pretreatment of urine

[0046] The research team of the present invention collected 24-hour urine from 36 stone patients and 11 healthy people from West China Hospital o...

Embodiment 2

[0053] Feasibility Analysis of Oxalic Acid Detection

[0054] Quantum dots were characterized in several ways using transmission electron microscopy, UV absorption-visible spectrometer and fluorescence spectrophotometer. The synthesized quantum dots have a spherical shape and an average particle size of 4 nm ( figure 2 a). The ultraviolet absorption and fluorescence emission characteristic peaks ( figure 2 b).

[0055] Subsequently, the effect of oxalic acid on the fluorescence signal of quantum dots was detected. In the range of 0 to 1 mmol / L, oxalic acid had no significant effect on the fluorescence signal of quantum dots ( figure 2 E), exhibits a low interference signal similar to that of ascorbic acid.

[0056] Compared with the effect of oxalic acid on quantum dots, Cu 2+ It shows obvious quenching effect on the fluorescence signal of quantum dots ( figure 2 f-d compared to 2f-b and c). Add oxalic acid at a concentration of 1 to 100 micromolar per liter to Cu ...

Embodiment 3

[0058] This embodiment is a preferred embodiment. Optimized conditions that affect assay performance, including Cu 2+ Reaction time with oxalic acid, Cu + and quantum dot reaction time, Cu + and the incubation time of quantum dots, and the volume of quantum dots.

[0059] As the reaction time prolongs, the fluorescence signal of quantum dots decreases rapidly, indicating that oxalic acid reduces Cu 2+ Can be done in 3 minutes ( image 3 a). Cu + The quenching reaction to quantum dots is fast and can be completed within 2.5 minutes ( image 3 b). As the volume of quantum dots increased, the fluorescence signals of solutions with and without oxalic acid increased ( image 3 c). At 1 microliter of quantum dots, the above solution obtained the maximum fluorescence signal difference ( image 3 d). Therefore, in the follow-up experiments, 3 min was used for oxalic acid reduction of Cu 2+ , 2.5 min was adopted for quenching QDs, and 1 µl QDs as signaling molecules.

[00...

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Abstract

The invention belongs to the field of chemical detection, and particularly relates to a method for detecting oxalic acid by using quantum dots, which is characterized by comprising the following steps: (1) preparing a detection solution, adding a bivalent copper ion solution and a buffer solution into the detection solution, and incubating to obtain a first reaction solution; (2) adding a quantum dot solution into the first reaction solution, and incubating to obtain a second reaction solution; and (3) detecting the concentration of oxalic acid in the detection solution by measuring a fluorescence spectrum. The invention provides a simple, high-sensitivity, rapid, low-cost, quantitative and specific method for detecting the oxalic acid content, and the method has a wide application prospect in the fields of clinical diagnosis (such as caluria oxalate), environment, chemical industry and the like.

Description

[0001] This application may serve as the priority basis for subsequent patent applications (including, but not limited to, Chinese invention patent applications, Chinese utility model applications, PCT applications, and foreign applications based on the Paris Convention). technical field [0002] The invention belongs to the field of chemical detection, and in particular relates to a method and application for detecting oxalic acid by using quantum dots. Background technique [0003] Urolithiasis is a common disease affecting 5% to 8.8% of the global population with a 5-year recurrence rate of up to 50%. Urolithiasis mainly includes calcium oxalate and calcium urate, and calcium oxalate stones are the most common type of urolithiasis. Urolithiasis can easily form when urinary oxalate levels are supersaturated. Oxalate in urine may be derived from diet or endogenous synthesis in the liver. In clinical practice, measurement of urinary oxalate content in stone formers can ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58
CPCG01N33/588G01N2800/345
Inventor 耿佳陈飘飘应斌武白云金
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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