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Preparation method of nanofiber modified nitrocellulose immunochromatography membrane

A nitrocellulose and nanofiber technology, applied in the field of material science, can solve problems such as unsuitable for on-site detection, time-consuming, and complicated, and achieve the effects of uniform thickness, improved sensitivity, and improved adsorption performance

Pending Publication Date: 2021-07-30
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods to improve the sensitivity of LFA require additional equipment, complicated operations or specialized personnel, and most of them are time-consuming and not suitable for on-site detection.

Method used

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  • Preparation method of nanofiber modified nitrocellulose immunochromatography membrane
  • Preparation method of nanofiber modified nitrocellulose immunochromatography membrane
  • Preparation method of nanofiber modified nitrocellulose immunochromatography membrane

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Experimental program
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Effect test

Embodiment 1

[0043] In the present invention, the immunochromatographic membrane is obtained through the electrospinning method with simple operation, which is specifically implemented according to the following steps:

[0044] Step 1, accurately weigh nitrocellulose, wherein the mass percentage of nitrocellulose is 18%;

[0045] Step 2, accurately measure highly volatile polar organic solvent and high conductivity polar solvent, wherein the volume ratio of organic solvent and polar solvent is 1:1;

[0046] Step 3. Preparation of spinning solution: add nitrocellulose, highly volatile polar organic solvent, and high conductivity polar solvent into a small beaker, stir at room temperature for 24 hours, and obtain a light yellow transparent clear solution for spinning ;

[0047] Step 4. Electrospinning operation: Ultrasonicate the solution for 0.5h in an ultrasonic cleaner until the bubbles in the liquid are completely removed. Carefully place the commercially available NC membrane on the fla...

Embodiment 2

[0050] In the present invention, the immunochromatographic membrane is obtained through the electrospinning method with simple operation, which is specifically implemented according to the following steps:

[0051] Step 1, accurately weigh nitrocellulose, wherein the mass percentage of nitrocellulose is 22%;

[0052] Step 2, accurately measure highly volatile polar organic solvent and high conductivity polar solvent, wherein the volume ratio of organic solvent and polar solvent is 5:4;

[0053] Step 3. Preparation of spinning solution: respectively put nitrocellulose, highly volatile polar organic solvent, and high conductivity polar solvent in a small beaker, and stir at room temperature for 24 hours to obtain a light yellow transparent clear solution, which is ready for spinning;

[0054] Step 4. Electrospinning operation: Ultrasonicate the solution for 0.5h in an ultrasonic cleaner until the bubbles in the liquid are completely removed. Carefully place the commercially ava...

Embodiment 3

[0057] In the present invention, the immunochromatographic membrane is obtained through the electrospinning method with simple operation, which is specifically implemented according to the following steps:

[0058] Step 1, accurately weigh nitrocellulose, wherein the mass percentage of nitrocellulose is 25%;

[0059] Step 2, accurately measure highly volatile polar organic solvent and high conductivity polar solvent, wherein the volume ratio of organic solvent and polar solvent is 5:3;

[0060] Step 3. Preparation of spinning solution: respectively put nitrocellulose, highly volatile polar organic solvent, and high conductivity polar solvent in a small beaker, and stir at room temperature for 24 hours to obtain a light yellow transparent clear solution, which is ready for spinning;

[0061] Step 4. Electrospinning operation: Ultrasonicate the solution for 0.5h in an ultrasonic cleaner until the bubbles in the liquid are completely removed. Carefully place the commercially ava...

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Abstract

The invention relates to a preparation method of a nanofiber modified nitrocellulose immunochromatography membrane. The method comprises the following steps of adding nitrocellulose, an organic solvent and a polar solvent into a container, and performing uniform stirring to obtain a spinning solution; and carefully placing a commercially available NC membrane on a flat plate receiver, starting an instrument to perform spinning for 0-4 h, and drying the spun membrane. The immunochromatography membrane is obtained by a simple one-step spinning method; immunochromatography membranes with different porosities, protein adsorption capacities and flow velocities are obtained by controlling spinning time and regulating spinning parameters; and the purpose of improving the sensitivity, specificity and repeatability of detecting substances such as protein and DNA is achieved when the immunochromatography membrane is applied to an immunochromatographic test strip.

Description

technical field [0001] The invention belongs to the technical field of material science, and in particular relates to a preparation method of a nanofiber-modified nitrocellulose immunochromatographic membrane. Background technique [0002] Lateral flow analysis (LFA) test strips are small in size, easy to carry, and short in detection time. The pharmaceutical reagents used are stable and easy to store. They do not need large-scale equipment for qualitative detection, and can be detected by simple reading and analysis instruments. For semi-quantitative or quantitative detection, it is an analytical method very suitable for medical diagnosis, and has a wide range of applications in many fields such as food safety, water resources, medical safety, and life and health. Currently, several common LFA test strips, such as ovulation test strips, pregnancy test strips, and urine test strips, are sold in hospitals and pharmacies, and have become successful cases of LFA commercializati...

Claims

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Application Information

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IPC IPC(8): D04H1/4258D04H1/4382D04H1/728D01D5/00G01N33/531G01N33/558
CPCD04H1/4258D04H1/4382D04H1/728D01D5/003D01D5/0092D01D5/0069G01N33/531G01N33/558
Inventor 薛朝华王雪杨冬
Owner SHAANXI UNIV OF SCI & TECH
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